Gene vector and preparation method thereof

Abstract

The invention discloses a gene vector and a preparation method thereof, and relates to the technical field of gene vectors. The gene vector comprises a magnetic core and a tannic acid film coated on the surface of the magnetic core, wherein the magnetic core is magnetic nano-particle ferroferric oxide. The preparation method of the gene vector includes adding anhydrous ferric chloride and anhydrous sodium acetate into ethylene glycol, and heating and stirring to form a transparent solution; placing the transparent solution in an autoclave and heating until magnetic nanoparticles are formed; dissolving the magnetic nanoparticles, ferric chloride and tannic acid in water to form a mixed solution; and after the mixed solution is adjusted to be neutral, stirring the solution until the tannic acid film is fully coated on the surfaces of the magnetic nanoparticles, magnetic separating, washing and drying to obtain the gene vector. The gene vector provided by the method has good stability andlow cytotoxicity.

Description

technical field [0001] The invention relates to the technical field of gene carriers, in particular to a gene carrier and a preparation method thereof. Background technique [0002] In addition to the safe and effective target gene, the success of gene therapy is also extremely important how to introduce the target gene into cells and express it successfully. The naked foreign gene DNA is difficult to enter the cell and is easily degraded by the body or cells, so it is difficult to express. Therefore, a carrier with high transfection rate and low toxicity is required to assist the target gene to enter the target cell. Here, it is used to transfer the target gene into the target cell. The carrier is the gene carrier. [0003] Inorganic nanoparticles can form nanomaterials and nucleic acid complexes by adsorbing nucleic acids, thereby protecting DNA molecules from degradation by nucleases. They are currently a relatively common type of non-viral gene carrier, but inorganic na...

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Problems solved by technology

[0003] Inorganic nanoparticles can form nanomaterials and nucleic acid complexes by adsorbing nucleic acids, thereby protecting DNA molecules from degradation by nucleases. They are currently a relatively common type of non-viral gene carrier, but inorganic nanoparticles have greater cytotoxicity, and Most inorganic nanoparticles will lose their stability after being placed for a long time, and are prone to agglomeration, resulting in the loss of physical and chemical properties

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