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Method for reducing proliferation of resistance gene in anaerobic treatment of sludge caused by nano material

A nanomaterial and anaerobic treatment technology, applied in the field of environmental protection, can solve the problem of ignoring the potential effect of nanomaterial resistance genes, and achieve the effect of significant reduction effect, simple operation and cost reduction.

Inactive Publication Date: 2019-05-28
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies have ignored the potential role of nanomaterials in resistance genes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) After anaerobic digestion of the sludge containing nano-copper oxide to be treated, the sulfonamide resistance genes sul I and sulII increased by 7.5 times and 3.2 times, respectively, and the tetracycline resistance gene tet Q increased by 8.2 times. The sex gene mex B increased by 9.0 times.

[0031] (2) Perform anaerobic pre-fermentation on the protein product wastewater, control the process temperature to 30 degrees, the pH of the solution to 8, and the anaerobic fermentation time to 2 days.

[0032] (3) Mix the solution obtained by fermentation with the sludge containing nano-copper oxide, and control the concentration of cysteine ​​in the solution to 10 μmol / L.

[0033] (4) Adjust the pH of the mixture to 7.0. After 16 days of anaerobic digestion, the sulfonamide resistance genes sul I and sul II increased by 0.2 times and 0.15 times respectively, the tetracycline resistance gene tet Q did not increase, and the multiple resistance gene mex B increased by 0.05...

Embodiment 2

[0035] (1) After anaerobic digestion of the sludge containing nano-zinc oxide to be treated, the sulfonamide resistance genes sul I and sulII increased by 5.2 times and 2.8 times respectively, and the tetracycline resistance gene tet Q increased by 7.5 times. The sex gene mex B increased by 7.6 times.

[0036] (2) Perform anaerobic pre-fermentation on the high-sulfur sludge, control the process temperature to 37 degrees, the pH of the solution to 9, and the anaerobic fermentation time to 1 day.

[0037] (3) Mix the solution obtained by fermentation with the sludge containing nano zinc oxide, and control the concentration of cysteine ​​in the solution to 15 μmol / L.

[0038] (4) Adjust the pH of the mixture to 7.2. After 20 days of anaerobic digestion, the sulfonamide resistance genes sul I and sul II increased by 0.18 times and 0.11 times respectively, the tetracycline resistance gene tet Q did not increase, and the multiple resistance gene mex B increased by 0.09 times.

Embodiment 3

[0040] (1) After anaerobic digestion of the nano-copper-containing sludge to be treated, the sulfonamide resistance genes sul I and sul II increased by 6.8 times and 4.5 times, respectively, and the tetracycline resistance gene tet Q increased by 8.8 times. The sex gene mex B increased by 9.9 times.

[0041] (2) Carrying out anaerobic pre-fermentation of livestock and poultry feces, controlling the temperature of the process to be 35 degrees, the pH of the solution to be 8.5, and the anaerobic fermentation time to be 3 days.

[0042] (3) The fermented solution was mixed with the nano-copper-containing sludge, and the concentration of cysteine ​​in the solution was controlled to be 9 μmol / L.

[0043] (4) Adjust the pH of the mixture to 6.8. After 18 days of anaerobic digestion, the sulfonamide resistance genes sul I and sul II increased by 0.20 times and 0.19 times respectively, the tetracycline resistance gene tet Q increased by 0.15 times, and the multiple resistance gene mex...

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Abstract

The invention discloses a method for reducing proliferation of resistance genes in anaerobic treatment of sludge caused by a nano material. According to the method, the biological availability of thenano-material is reduced by utilizing related substances, the biological chemical effect of the nano material on related microorganisms is weakened, and therefore the proliferation of the resistance genes in the anaerobic digestion process is greatly reduced. The method comprises the following steps of obtaining a cysteine-containing solution; uniformly proportioning the obtained solution and thesludge containing the nano material; and carrying out anaerobic treatment on the uniformly-mixed sludge, wherein organic wastewater / waste is protein product wastewater, high sulfur-containing sludge or excrements of livestock and poultry and other sulfur-containing waste organic matter. The method has the advantages that the proliferation of the resistance genes can be effectively controlled, andcompared with conventional anaerobic treatment in which the sludge resistance genes are increased by 2-10 times, the proliferation of the resistance genes caused by the nano material can be controlledwithin 0-0.2 times; in addition, the cost for controlling the proliferation of the resistance genes is remarkably lowered while waste recycling is realized; and meanwhile, the operation is simple andconvenient, and the like.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and relates to a new method for controlling the multiplication of resistance genes in the process of anaerobic treatment of sludge containing nanomaterials by using sulfur-containing organic waste to pre-ferment to produce a solution containing cysteine. Background technique [0002] As we all know, nanomaterials are widely used in surface coatings, pharmaceuticals, cosmetics, catalysts and other industries due to their excellent physical and chemical properties. However, during the production and use of nanomaterials, nanomaterials will inevitably leak into the natural environment and enter the sewage treatment plant along with the drainage collection system. At this stage, conventional sewage treatment processes cannot effectively remove nanomaterials. A large number of studies have confirmed that nanomaterials will adsorb and accumulate in sludge, which will affect the subseq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/04
Inventor 陈银广黄海宁郑雄卜凡
Owner TONGJI UNIV
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