Microfluidic chip for cell culture and culture method

Abstract

The invention relates to a microfluidic chip for cell culture and belongs to the technical field of microfluidics. The microfluidic chip includes a chip layer, and the upper surface of the chip layeris provided with a cell culture chamber, an inlet pool, an inlet channel, an outlet pool and an outlet channel. The cell culture chamber is a large recessed area disposed on the upper surface of the chip layer, the inlet channel and the outlet channel are grooves disposed on the upper surface of the chip layer, and the inlet channel and the outlet channel are small recessed areas disposed on the upper surface of the chip layer. The inlet pool is in communication with the inlet channel and is in communication with the cell culture chamber through the inlet channel, the cell culture chamber is in communication with the outlet pool through the outlet channel, the inlet pool is used for connection with a fresh medium conveying pipe, and the outlet pool is used for connection with a cell culture metabolic waste liquid discharging pipe. The chip for cell culture by using the method has the advantages of less sample consumption, high culture efficiency, simple operation, high cell survival rate, and the like.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and in particular relates to a microfluidic chip for cell culture, a culture method, and a cell culture device. Background technique [0002] In order to better imitate the microenvironment in which cells live in the human body, only by creating a co-culture system of multiple cells can the partial functions of human organs be reconstructed in vitro. Therefore, there is an urgent need for a new technique to co-culture several types of cells. Cell co-culture is an important means to study the interaction between cells. At present, there are mainly the following methods for cell co-culture research: 1) Mixed culture; 2) Conditioned medium culture; 3) Microcarrier culture method; 4) Microwell Bottom membrane dish culture method. All of the above methods have problems such as large reagent consumption and inaccurate flow rate control, and are only suitable for studying the biological behavior...

AI Technical Summary

Problems solved by technology

All of the above methods have problems such as large reagent consumption and inaccurate flow rate control, and are only suitable for studying the biological behavior of cells on a two-dimensional plane. It is difficult to imitate the complex microenvironment in vivo, which seriously affects the experimental results.

[0003] Traditional cell culture methods, such as placing cells in culture dishes or flasks, cannot simulate the liquid flow environment of cells in actual conditions, and require frequent replacement of culture media to waste manpower and material resources; while conventional microfluidic cell culture methods require Using large equipment such as peristaltic pumps and incubators often requires a long pipeline between the chip and these equipment, which greatly affects the performance of cell culture experiments.

Images