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Application of chitooligosaccharide in preparation of drugs for regulating human or animal intestinal flora disorders

A technology of intestinal flora and chitosan oligosaccharides, which is applied in the direction of drug combinations, pharmaceutical formulas, and medical preparations containing active ingredients, etc., to achieve intestinal mucosal barrier repair, intestinal flora disorder, and significantly regulate the intestinal tract The effect of bacteria

Pending Publication Date: 2019-06-04
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that chitooligosaccharides can significantly change the bacterial composition of human gut microbes cultured in vitro, but the regulation of chitooligosaccharides on the disturbance of intestinal flora in animals has not been reported in the literature or found in patents

Method used

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  • Application of chitooligosaccharide in preparation of drugs for regulating human or animal intestinal flora disorders
  • Application of chitooligosaccharide in preparation of drugs for regulating human or animal intestinal flora disorders
  • Application of chitooligosaccharide in preparation of drugs for regulating human or animal intestinal flora disorders

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Chitooligosaccharides regulate intestinal flora

[0021] (1) In this example, in order to evaluate the regulatory effect of chitosan oligosaccharides on the intestinal flora of obese mice, 24 four-week-old C57BL / 6 mice were randomly divided into 4 groups, 6 in each group, respectively Corresponding to normal diet group (normal group), high-fat diet group (model group), normal diet and chito-oligosaccharide combined group, high-fat diet and chito-oligosaccharide combined group, etc. The mice were reared in an environment with constant temperature and humidity, and underwent an adaptation period of 1 week. Feed the corresponding normal feed or high-fat feed respectively, and at the same time feed the dose of chitosan oligosaccharide (1000Da) 100mg / kg to water, and the feeding process lasts for 5 months.

[0022] (2) After step (1) is completed, collect fresh feces in the large intestine of the mouse, extract the genes therein, and perform second-generation high...

Embodiment 2

[0027] Example 2 Chitooligosaccharides regulate intestinal flora

[0028] (1) In this example, in order to evaluate the regulatory effect of chitosan on the intestinal flora of diabetic mice, 12 four-week-old transgenic diabetic (db / db) mice and 12 four-week-old wild The mice were randomly divided into 4 groups, 6 in each group, corresponding to the normal group, the diabetes model group, the chitosan oligosaccharide intervention normal group, the chitosan oligosaccharide intervention diabetes group, etc. The dose of chitooligosaccharide (1000Da) 300mg / kg was fed to water, and the feeding process lasted for 3 months.

[0029] (2) After step (1) is completed, collect fresh feces in the large intestine of the mouse, extract the genes therein, and perform second-generation high-throughput nucleic acid sequencing based on the amplified fragment of the V3-V4 region of 16SrRNA, and compare the database information. Combining bioinformatics analysis and statistical analysis, the typ...

Embodiment 3

[0033] The application method of embodiment 3 chitooligosaccharides

[0034] (1) tablet

[0035] a. Take a certain amount of chitosan oligosaccharide dry powder and dissolve it in water to form a chitosan oligosaccharide solution;

[0036] b. Add an appropriate amount of conventional auxiliary materials to the chitooligosaccharide solution obtained in step a, and mix well;

[0037] c. The homogeneous mixed solution obtained in step a is made into granules by high-speed wet method, then boiled and dried at 60 degrees, pressed into a certain quality sheet, and packaged separately to obtain.

[0038] (2) Granules

[0039]a. Take a certain amount of chitosan oligosaccharide dry powder and dissolve it in water to form a chitosan oligosaccharide solution;

[0040] b. Add an appropriate amount of conventional auxiliary materials to the solution obtained in step a and stir into mud;

[0041] c. Extrude and granulate the obtained product in step b through a 20-mesh sieve, boil and ...

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Abstract

The invention belongs to the fields of health-care food and food science and technology, and in particular, relates to an application of chitooligosaccharide in regulating animal intestinal disorders.The chitooligosaccharide is found out to be able to regulate the intestinal flora disorders caused by obesity or diabetes, promote the growth of beneficial bacteria such as intestinal lactobacillus and bifidobacterium, improve the intestinal mucosal barrier and significantly inhibit the proliferation of harmful bacteria such as vibrio desulfurizans. The invention provides an application method ofmaking chitooligosaccharide into clinically accepted pharmaceutical preparations or health-care food by a conventional process. The chitooligosaccharide has positive application value for preparing drugs for preventing and treating intestinal disorders of diabetes, obesity and intestinal inflammation.

Description

technical field [0001] The invention belongs to the technical field of health food and food, and in particular relates to the application of chitosan oligosaccharide in regulating the disorder of animal intestinal flora. Background technique [0002] Gut flora is the general term for the bacterial community that inhabits the intestinal tract. The bacterial species in the intestinal flora are complex, containing more than 40,000 strains, and the number of bacteria reaches 10 14 , is the number of human cells (10 13 ), which can reach 1 to 2 kilograms by weight, which is equivalent to the weight of an adult brain. Intestinal flora parasitizes in the host's intestines, shares food with the host, and co-exists with each other. Its own or metabolites can directly affect or regulate the host's glucose and lipid metabolism, immunity, nerve excitation and other important processes. Studies have shown that the composition of the intestinal flora is closely related to the diseases ...

Claims

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Application Information

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IPC IPC(8): A61K31/702A61K31/722A61P1/00
Inventor 尹恒郑军平刘洪涛程功赵小明杜昱光
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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