Artemia sinica separation method

Abstract

The invention belongs to the field of biological product processing, and particularly relates to an artemia sinica separation method which includes the steps: performing static settlement for 10 minutes; collecting artemia sinica and parts of mixed unhatched dead eggs and empty shells; soaking the artemia sinica, the unhatched dead eggs and the empty shells with potassium permanganate solution for30 seconds; adding fresh water for dilution and then immediately adding hydrogen peroxide; homogenizing a water body, replenishing hydrogen peroxide, removing upper empty shells and dead eggs 3 minutes after and repeating the operation once to twice according to separation degree; collecting the separated artemia sinica in a centralized manner. Based on deficiencies of a floating separation method and a magnetic powder separation method, the advantages of two separation methods are combined, and the artemia sinica separation method is economical, simple, efficient, rapid, wide in applicability and less in artemia body damage.

Description

technical field [0001] The invention belongs to the field of biological product processing, in particular to a method for separating Artemia nauplii. Background technique [0002] Artemia nauplii is an important biological bait in the process of contemporary aquatic breeding. Because of its high nutritional value, especially rich in unsaturated fatty acids, good palatability and easy absorption, it becomes the larvae of aquatic animals such as shrimp, crab and fish. The excellent bait has been widely used in seedling production. Artemia eggs can hatch into nauplii under suitable conditions of temperature, salinity, light and dissolved oxygen. The hatching rate of different eggs is different, and the content of unhatched dead eggs is also different. When the Artemia eggs and Artemia nauplii are not completely separated and the aquatic seedlings are fed, a large number of Artemia eggs will be brought into the nursery pond, which will provide the attachment base for the produc...

AI Technical Summary

Problems solved by technology

When the Artemia eggs and Artemia nauplii are not completely separated and the aquatic seedlings are fed, a large number of Artemia eggs will be brought into the nursery pond, which will provide the attachment base for the production and reproduction of pathogenic bacteria and polycondensate parasites, and some Artemia The eggs will be ingested by the seedlings, unable to digest and absorb, and even accumulate in the intestinal tract, causing the seedlings to die

[0003] There are the following four methods for the separation of common Artemia nauplii at present: (1) mechanical filtration method: filter with a specific mesh dragging net; the disadvantage of adopting mechanical filtration separation is: the egg diameter of Artemia eggs is usually 220-300mm. Micron, while the length of Artemia nauplii is 500-700 microns, and the width is 180-250 microns. Due to the differences in the size of Artemia eggs and nauplii of different strains, it is difficult to choose a filter screen with suitable aperture; filter screen If the pore size is too large, unhatched Artemia eggs can also pass through, and if the pore size of the filter screen is too small, some Artemia nauplii cannot pass through the filter screen; moreover, the Artemia nauplii are not smooth and round, and the extrusion and punching during filtering Drenching will damage the nauplii, causing a large number of artemia nauplius appendages to break and nutrients to lose

(2) Light-induced method: that is, after Artemia hatching is completed, the larvae are lured by light-induced method, and then siphoned to obtain the larvae; this method is time-consuming and has poor separation effect, which cannot meet the needs of production. The purity of Artemia nauplii generally can only reach about 75%. After the Artemia larvae mixed with a large number of unhatched Artemia eggs and egg shells are put into the nursery pool, the unhatched Artemia eggs and egg shells will be in the nursery pool. A large amount of accumulation will cause great harm to the seedlings of aquatic animals. For example, if the seedlings are swallowed, intestinal obstruction may occur and die, or the surface of the seedlings is easy to attach pathogenic organisms, such as filamentous bacteria and polyzoa, to infect the seedlings, and then the seedlings will appear. quality problem

(3) Air flotation separation method: mainly by adding chemical samples such as hydrogen peroxide or percarbonate, using the bubbles generated by the decomposition of the light to attach to the surface of unhatched eggs or empty shells, and relying on buoyancy to lift them to the liquid surface, without The nauplii get rid of the air bubbles and sink to the bottom through movement, so as to achieve the purpose of separating the nauplii, but the decomposition speed of hydrogen peroxide and percarbonate is slow, and the separation takes a long time, and the air bubbles formed on the surface of unhatched eggs are susceptible to Affected by the movement of surrounding nauplii, a large number of unhatched dead eggs continue to be mixed in the middle of the nauplii and cannot be completely separated

(4) Magnetic particle separation method: when the eggs are processed, a layer of special magnetic powder is wrapped on the surface, and after the hatching is completed, the unhatched dead eggs and the hatched empty shells are collected by magnetic attraction, so as to achieve the purpose of separating the nauplii; The disadvantage of using the magnetic separation method is that it requires advanced technology to evenly coat the surface of the eggs with magnetic substances. At present, there are very few domestic companies with this technology, and the eggs are expensive. Most of the products in the market do not use this technology.

In addition, Chinese Patent Application No. 201610276290.1 ​​discloses a method for hatching and rapidly separating Artemia nauplii. The separation method uses a 120-mesh screened silk bag to repeatedly shake in a glass fiber reinforced plastic tank, so that the The nauplii are thrown into the FRP water tank to achieve the effect of separating the nauplii; this separation method can increase the separation speed of the larvae, but this method is essentially the same as the mechanical filtration method, and the nauplii will be damaged during the shaking and washing process , so that a large number of artemia nauplius appendages break, and nutrients are lost