Yak skin polypeptide with effect of affecting platelet aggregation and preparation method thereof

A technology of platelet aggregation and yak skin, which is applied in the field of medicine, can solve the problems of physical and chemical properties of yak skin glue - denaturation temperature, emulsification performance, foaming ability, foaming stability and anti-oxidation performance, etc., so as to improve economic added value, Extensive sources of raw materials and rich nutrition

Inactive Publication Date: 2019-06-14
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous research has found that yak hide glue with different molecular weights has great differences in physical and chemical prope

Method used

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  • Yak skin polypeptide with effect of affecting platelet aggregation and preparation method thereof
  • Yak skin polypeptide with effect of affecting platelet aggregation and preparation method thereof
  • Yak skin polypeptide with effect of affecting platelet aggregation and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0033] A yak skin polypeptide with the effect of affecting platelet aggregation, the molecular weight is composed of 17KDa, 34 KDa, 43 KDa, the maximum ultraviolet absorption wavelength of the peptide bond is at 216 nm, and the infrared spectrum of the amide I band is at 1600~1700 cm -1 , the amide II band infrared spectrum is at 1560~1500 cm -1 At 1234~1237 cm in the infrared spectrum of amide III -1 place.

[0034] A preparation method of a yak skin polypeptide having the function of affecting platelet aggregation, comprising the following steps:

[0035] (1) Microdermabrasion: Clean the Qinghai-Tibet Plateau yak hide after depilation and degreasing, cut it into 10cm squares with a cutter, put it in a microdermabrasion machine to grind off the epidermis, and clean it;

[0036] (2) Heating: add the yak skin with the skin removed to the glycine buffer solution with a pH of 1 and a concentration of 0.1 mol / L at a ratio of 1:1, heat in microwave for 2 min, and cool to room tem...

Embodiment 2

[0044] A yak skin polypeptide with the effect of affecting platelet aggregation, the molecular weight is composed of 17KDa, 34 KDa, 43 KDa, the maximum ultraviolet absorption wavelength of the peptide bond is at 216 nm, and the infrared spectrum of the amide I band is at 1600~1700 cm -1 , the amide II band infrared spectrum is at 1560~1500 cm -1 At 1234~1237 cm in the infrared spectrum of amide III -1 place.

[0045] A preparation method of a yak skin polypeptide having the function of affecting platelet aggregation, comprising the following steps:

[0046] (1) Microdermabrasion: Clean the Qinghai-Tibet Plateau yak hide after depilation and degreasing, cut it into 15cm squares with a cutter, put it in a microdermabrasion machine to grind off the epidermis, and clean it;

[0047] (2) Heating: add the yak hide with the skin removed to the glycine buffer solution with a pH of 9 and a concentration of 0.1 mol / L at a ratio of 1 to 10, heat in microwave for 30 min, and cool to roo...

Embodiment 3

[0055] A yak skin polypeptide with the effect of affecting platelet aggregation, the molecular weight is composed of 17KDa, 34 KDa, 43 KDa, the maximum ultraviolet absorption wavelength of the peptide bond is at 216 nm, and the infrared spectrum of the amide I band is at 1600~1700 cm -1 , the amide II band infrared spectrum is at 1560~1500 cm -1 At 1234~1237 cm in the infrared spectrum of amide III -1 place.

[0056] A preparation method of a yak skin polypeptide having the function of affecting platelet aggregation, comprising the following steps:

[0057] (1) Microdermabrasion: Clean the Qinghai-Tibet Plateau yak hide after depilation and degreasing, cut it into 13cm squares with a cutter, put it in a microdermabrasion machine to grind off the epidermis, and clean it;

[0058] (2) Heating: add the yak hide with the skin removed into the glycine buffer solution with a pH of 4 and a concentration of 0.1 mol / L at a ratio of 1:5, heat in microwave for 15 min, and cool to room ...

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Abstract

The invention relates to the field of medicines, in particular to yak skin polypeptide with an effect of affecting platelet aggregation and a preparation method thereof. The yak protein is composed of17KDa, 34 KDa and 43 KDa in molecular weight. The maximum ultraviolet absorption wavelength of a peptide bond is at the 216nm position, the infrared spectrum of an amide I band is at the 1600-1700 cm-1 position, the infrared spectrum of an amide II band is at the 1560-1500cm position, and the infrared spectrum of the amide III is at the 1234-1237 cm-1 position. The preparation method includes buffing, heating, foaming, enzymatic hydrolysate preparation, centrifugal concentration and separation and drying. The pure natural pollution-free Qinghai-Tibet Plateau yak skin is used as the main raw material, the raw material source is wide, and the nutrition is rich. A product with the effect of affecting platelet aggregation is increased while the economic value added of the yak skin is increased. The polypeptide is simple in preparation and operation process. The prepared yak skin polypeptide has three molecular weight segments of bioactive components affecting the platelet aggregation, andthe platelet aggregation rate in vitro of mice is 38.4%.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a yak skin polypeptide with the effect of affecting platelet aggregation and a preparation method thereof. Background technique [0002] The yak is a unique cattle species on the plateau. It lives in the harsh living environment of hypoxia, high cold, high altitude, low and sparse pasture, and long dry grass period. The glue made from its fresh hide is rich in nutrients. It is reported in the literature that yak hide glue contains 20.95% of essential amino acids and mineral elements such as calcium, sodium, magnesium and potassium. Previous research has found that yak hide glue with different molecular weights has great differences in physical and chemical properties, such as denaturation temperature, emulsification performance, foaming ability and foaming stability, and oxidation resistance. Therefore, the development of yak leather products can not only increase the economic value of ...

Claims

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Application Information

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IPC IPC(8): C07K2/00C12P21/06C07K1/34A61K38/01A61P7/04
Inventor 杜玉枝李岑肖远灿毕宏涛张明杨红霞魏立新
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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