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Plant urease sand solidification method

A sand-fixing method and plant urease technology are applied in chemical instruments and methods, hydrolase, fertilizer mixture and other directions, which can solve the problems of low activity of soybean urease, flammable acetone solvent, complicated operation process, etc., so as to improve the curing effect and be environmentally friendly. , the effect of simple process

Inactive Publication Date: 2019-06-14
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some researchers use organic solvents such as ethanol and acetone to extract urease from soybeans, such as "Extraction and Activity Determination of Soybean Urease" by Lin Liyun et al. ("Food Research and Development", 2013, Vol.34, No.9, 85- 88), the published patent CN103601602A "Extraction method and application of urease-inhibiting components in privet leaves", but the activity of soybean urease extracted by ethanol and acetone is low, the operation process is cumbersome, and the acetone solvent is flammable and toxic, and is Controlled chemicals are not conducive to the extraction of urease in large quantities at the actual engineering site

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for preparing calcium carbonate by directly extracting plant urease from soybeans, the steps are as follows:

[0021] 1) Preparation of soybean flour: crush soybeans, pass through a 100-mesh sieve to obtain soybean flour, refrigerate at 4°C, and set aside;

[0022] 2) Extract urease: mix the two according to the volume ratio of soybean powder and distilled water at 1:10, add 1% zeolite powder at the same time, shake well, store at 4°C for 24 hours, and use 3000 r / min After centrifugation for 15 min, the supernatant obtained by filtration is the urease solution, and the activity of urease is determined;

[0023] 3) Take the urease solution obtained in step 2) to prepare a urease solution with an activity of 4000U / L, take 50mL of the urease solution, add 3g of bentonite and stir evenly, and spray it on the tray covered with aeolian sand; then spray 50mL to gel liquid. Wherein, the gelling solution is a mixed solution of 0.75 mol / L urea and 0.75 mol / L calcium ac...

Embodiment 2

[0025] Embodiment 2 compares the impact of different processes on the calcium carbonate precipitation yield

[0026] Schemes 1-7 control different solid-liquid ratios (the solid-liquid ratios mentioned here refer to step 2, the mass ratio of soybean flour to distilled water volume) and contrast the concentration of gelling liquid to study the influence of different processes on the precipitation yield of calcium carbonate.

[0027] In step (2), the two are mixed according to the mass ratio of soybean flour to distilled water volume of 1:6, 1:8, 1:10, 1:12, 1:14.

[0028] In step (3), 50mL of urease extract was sprayed on the surface of aeolian sand, only 3g of bentonite was added (the urease extract was not formulated to a specific activity), and then 50mL of different gelling solutions were sprayed, and the rest were the same as in Example 1 , the results are shown in Table 1:

[0029] Table 1

[0030]

[0031] The results in Table 1 show that the concentration of the ge...

Embodiment 3

[0032] Embodiment 3: compare the impact of different extraction solvents on the calcium carbonate precipitation yield

[0033] Schemes 1, 2, and 3 compare the effects of different extraction solvents on the yield of calcium carbonate precipitation.

[0034] In step (2), the two are mixed according to the mass ratio of soybean flour to different extraction solvents at a volume ratio of 1:10.

[0035] In step (3), 50 mL of urease extract was sprayed on the surface of aeolian sand, and only 3 g of bentonite was added (the urease extract was not prepared into a specific activity), and the rest of the content was the same as in Example 1. The results are shown in Table 2. It can be seen that adding The yield of calcium carbonate precipitation obtained by extracting urease with distilled water of zeolite powder is higher than that of 30% ethanol and 32% acetone, and distilled water is used as an extraction solvent, which is economical and environmentally friendly, and is convenient ...

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Abstract

The invention relates to a plant urease sand solidification method. The plant urease sand solidification method comprises following steps: soybean is crushed, distilled water containing zeolite powderis taken as a solvent for extraction of urease from soybean powder, a tray is covered with aeolian sand, a urease solution and a gel solution are sprayed onto the tray at a volume ratio of 1:1, ureais converted into CO<3><2-> and NH<4><+> through hydrolysis, the CO<3><2-> obtained through hydrolysis and Ca<2+> in a solution are combined to generate calcium carbonate, so that a hardened layer isformed on the surface of aeolian sand, and blowing by wind is prevented. The plant urease sand solidification method is simple in operation; urease generation complex process and harsh requirements inbacteria culture and breeding process are avoided, plant urease is adopted directly in catalysis of necessary reaction, carbonate deposition is induced, calcium carbonate extraction rate is high, requirements on the environment are low, and it is convenient for popularization and applications.

Description

technical field [0001] The invention belongs to a method for plant urease to induce calcium carbonate deposition, which can be used for dust prevention and sand fixation and environment improvement. Background technique [0002] Biodiagenetic and mineralization is a natural phenomenon, which has played an important role in the history of the formation of the earth, which is the result of geochemical research in the past thirty years. It is of great significance to the development of geotechnical engineering technology to re-understand the role and status of biodiagenesis and mineralization. The principle is that urease is produced during the growth and reproduction of microorganisms, which continuously decomposes urea to form CO 3 2- , in the negatively charged aqueous solution at the bacterial cell membrane interface, it continuously chelates Ca2+ in the environment with organic matter, and induces local crystal anions (CO32-), and as the CO32- concentration further incre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40E02D3/12C12N9/80
Inventor 缪林昌吴林玉孙潇昊林飞张继周
Owner SOUTHEAST UNIV
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