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Construction method for Atractylodes macrocephala Koidz. medical material UPLC characteristic graph and detection method

A construction method and technology of Atractylodes macrocephala, applied in the field of medicine, can solve the problems that cannot fully reflect the material characteristics of traditional Chinese medicine decoctions, have no content measurement items, and cannot comprehensively evaluate the quality of Atractylodes macrocephala, so as to improve the quality control level and stabilize Inner quality, the effect of improving inner quality

Inactive Publication Date: 2019-06-14
SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] "Chinese Pharmacopoeia" 2015 edition of Atractylodes atractylodes medicinal materials does not include content determination items, and cannot comprehensively evaluate the quality of Atractylodes atractylodes
At present, most of the fingerprints or characteristic spectra of Atractylodes macrocephala reported in the literature use the conventional HPLC method, and they only focus on the material basis of the original medicinal materials, and most of the index components are fat-soluble components as the research objects; they are mainly used for the qualitative identification of traditional Chinese medicinal materials. , cannot fully reflect the material basis characteristics of traditional Chinese medicine decoction

Method used

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  • Construction method for Atractylodes macrocephala Koidz. medical material UPLC characteristic graph and detection method
  • Construction method for Atractylodes macrocephala Koidz. medical material UPLC characteristic graph and detection method
  • Construction method for Atractylodes macrocephala Koidz. medical material UPLC characteristic graph and detection method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] Preparation of reference substance solution: take chlorogenic acid as reference substance, add solvent to dissolve, and prepare reference substance reference substance solution; take Atractylodes macrocephala control medicinal material, add extraction solvent to extract, filter, and take the subsequent filtrate as the reference medicinal material solution of reference substance;

[0032] Preparation of the test solution: take the Atractylodes Rhizome medicinal material, add extraction solvent to extract, filter, take the subsequent filtrate as the Atractylodes Rhizome medicinal material test solution; take the decoction pieces of Atractylodes Rhizome, add extraction solvent to extract, filter, and take the subsequent filtrate as the Atractylodes Rhizome decoction piece for testing Take bran-fried Atractylodes rhizome decoction pieces, add extraction solvent to extract, filter, take the subsequent filtrate as the test solution of bran-fried Atractylodes rhizome decoction p...

Embodiment 1

[0068] Example 1 Construction of Atractylodes macrocephala medicinal material UPLC characteristic map

[0069] 1. Instruments, reagents, reagents and medicinal materials

[0070] See Table 1 for instruments, Table 2 for reagents, Table 3 for reagents, and Table 4 for medicinal materials.

[0071] Table 1 Instrument information table

[0072] Instrument information

model

Suppliers

High performance liquid chromatography

H-class PDA

Waters Corporation

BEH C 18 Column

2.1*50mm, 1.7μm

Waters Corporation

BDS HYPERSIL C18 Column

2.1*50mm, 3μm

Thermo Fisher

one hundred thousandth of a balance

MS105DU

Mettler-Toledo

ten thousandth balance

AL104

Mettler-Toledo

ultrasonic cleaner

KQ-500DE

Kunshan Ultrasonic Instrument Co., Ltd.

[0073] Table 2 Reagent Information Sheet

[0074] Reagent information

batch number

Suppliers

phosphoric acid

B1214004 ...

Embodiment 2

[0191] Example 2: Determination of characteristic spectrum of Baizhu decoction pieces

[0192] Get 15 batches of Atractylodes Rhizome medicinal materials described in Table 4 in Example 1 to make Atractylodes Rhizome decoction pieces respectively according to the Pharmacopoeia method.

[0193] Preparation of Atractylodes Rhizome Decoction Pieces test solution: Take 1.0g of Atractylodes Rhizome Decoction Pieces powder (passed through a No. 3 sieve), accurately weigh it, put it in a flask, add 20ml of 30% (v / v) ethanol aqueous solution accurately, weigh it, and ultrasonically Treat (power 300W, frequency 40kHz) or reflux in a water bath for 30 minutes, let cool, weigh again, make up the lost weight with 30% (v / v) ethanol aqueous solution, shake well, filter, and take the subsequent filtrate to obtain Atractylodes macrocephala test product solution.

[0194] Get 15 batches of Atractylodes Rhizome decoction samples, each part of 1.0g, according to the preparation method of the ab...

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Abstract

The invention relates to a construction method for an Atractylodes macrocephala Koidz. medical material UPLC characteristic graph and a detection method. The construction method for the characteristicgraph comprises the following steps of preparing a comparison substance reference solution by taking chlorogenic acid as a comparison substance; preparing a comparison medical material reference solution by through utilization of an Atractylodes macrocephala Koidz. comparison medical material; obtaining the Atractylodes macrocephala Koidz. medical material, Atractylodes macrocephala Koidz. decoction pieces, bran-fried Atractylodes macrocephala Koidz. decoction pieces, Atractylodes macrocephala Koidz. standard decoction and bran-fried Atractylodes macrocephala Koidz. standard decoction, addingan extraction solvent for extraction, carrying out filtering, obtaining subsequent filtrate as a test sample solution; and injecting the reference solution and the test sample solution into an ultrahigh performance liquid chromatograph (UPLC) for measurement, calibrating a water soluble common peak, and obtaining the Atractylodes macrocephala Koidz. medical material UPLC characteristic graph. The method is good in reproducibility and is accurate and reliable. Through utilization of the constructed UPLC characteristic graph, material basis of the Atractylodes macrocephala Koidz. standard decoction can be reflected, and effective control over quality of the Atractylodes macrocephala Koidz. medical material can be realized. A quality monitoring level of the Atractylodes macrocephala Koidz.medical material is improved. A high quality stable Atractylodes macrocephala Koidz. raw material for a clinic preparation containing the Atractylodes macrocephala Koidz. can be provided.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a construction method and a detection method of a UPLC characteristic map of Atractylodes macrocephala medicinal material. Background technique [0002] Atractylodes macrocephala is the dried rhizome of Atractylodes macrocephala Koidz., a plant of Compositae, and it is a commonly used traditional Chinese medicine in clinic. It has the functions of invigorating the spleen and replenishing qi, drying dampness and promoting diuresis, antiperspirant, and anti-abortion. It is mostly used clinically to treat spleen deficiency, lack of food, abdominal distension and diarrhea, phlegm retention, dizziness, edema, spontaneous sweating, restless fetal movement, etc. It has strong Its clinical application value and market demand are broad, and it has been developed into related compound preparations such as Yupingfeng granules, and traditional Chinese medicine formula granules are widely us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张文芳林碧珊陈锦霞史紫娟曾杉陈伟钢甘伟发高永坚袁春平汤春花
Owner SINOPHARM GUANGDONG GLOBAL PHARMA CO LTD
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