Colorimetric detection method for primaquine drugs

A technology of primaquine and detection method, applied in the field of medicine, can solve the problems of interfering with PMQ determination and the like

Active Publication Date: 2019-06-21
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many endogenous biomolecules such as amino acids, vitamins, nucleic acids, and urochrome have strong absorption near 260 nm, which will interfere with the determination of PMQ

Method used

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  • Colorimetric detection method for primaquine drugs
  • Colorimetric detection method for primaquine drugs
  • Colorimetric detection method for primaquine drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The Griess reaction of embodiment 1 primaquine

[0035] Aniline and 1 equivalent of primaquine (PMQ) were dissolved in 5% phosphoric acid, and then sodium nitrite was slowly added at room temperature (25°C). The reaction can be easily monitored by visual observation or TLC following the formation of colored azo products (e.g. figure 1 ).

[0036] The reaction was performed using anilines having various substituents at the para position of the benzene ring. Changing substituent groups can form different colored products. Such as figure 2 As shown, substitutions with electron-donating effects can basically cause a red shift in the UV-Vis absorption spectrum. The spectrum of the strongly donating methoxy-substituted p-methoxyaniline reaction product 3d has the strongest absorption at 504nm, while the spectrum of the strongly electron-withdrawing sulfonamide product 3a has the strongest absorption at 470nm. The donating ability of methyl group is lower than that of me...

Embodiment 2

[0042] Embodiment 2 tests the change of colored product absorbance and time

[0043] PMQ can react with various anilines to generate colored products, and then use this color reaction to perform colorimetric determination of PMQ. Tests were performed with anilines with differently charged substituents (R) (sulfonyl 2a, methyl 2c and methoxy 2d). To test the absorbance versus time, 100 μL of aniline solution (200 mM in 0.2M HCl) was first mixed with 50 μL of PMQ (50 μM), followed by the addition of 50 μL of aqueous sodium nitrite (5 mM). The dynamic change of the absorbance of each aniline at the maximum wavelength was recorded by a multifunctional microplate reader.

[0044] Such as image 3 As shown, the reaction in 2a is faster than that of the other two anilines. The reaction rate of 2c is higher than that of 2d but lower than that of 2a, mainly because the electron-donating effect of the methyl group is intermediate between the two. As mentioned earlier, the same trend...

Embodiment 3

[0046] The selectivity of embodiment 3Griess reaction to PMQ

[0047] The antimalarial drugs 4-aminoquinoline mefloquine (MQ), chloroquine (CQ), piperaquine (PIP) and dihydroartemisinin (DHA) were selected to participate in the Griess reaction alone, or other antimalarial drugs were combined with PMQ respectively. Participate in the reaction after mixing. The result is as Figure 5 Shown, the absorbance value I 504 There was not any response to MQ, CQ, PIP and DHA (all 50 μM), but there was a response to the drug mixture containing PMQ (50 μM). The results showed that the Griess reaction-based approach was highly selective for PMQ and could distinguish other antimalarials commonly used in combination therapy for malaria.

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Abstract

The invention relates to a colorimetric detection method for primaquine drugs. In an acidic environment and in the presence of nitrite, para substituent-containing aniline can produce a Griess reaction with primaquine to form a colored azo product; a UV-Vis absorption spectrum and a maximum light absorption value of the colored product are determined; formation of the colored azo product and a change of a light absorption value thereof are detected in the presence of the primaquine with different concentrations by a microplate reader; a standard curve of the concentration of the primaquine andthe light absorption value of the azo product is established; and the concentration of the primaquine drugs in a to-be-tested sample is determined by a corresponding relation between the concentration of the primaquine and the light absorption value of the azo product on the standard curve. The method is successfully used for quantitatively analyzing and synthetizing the concentration of the primaquine in urine, and a detection limit is as low as 0.63 [mu]M. The method can also detect the primaquine from a serum sample within a clinically relevant concentration range.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a colorimetric detection method for primaquine drugs. Background technique [0002] Primaquine (PMQ) belongs to the structure of 8-aminoquinolines. It is the only antimalarial drug approved for the prevention and treatment of Plasmodium vivax and Plasmodium ovale. drug. However, the side effect of PMQ is that it often causes acute hemolytic anemia in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency, and the severity of hemolysis depends on the degree of G6PD deficiency, the dose and duration of PMQ medication. The frequency of G6PD gene mutations is 3-30% in malaria-endemic areas, and the distribution is wide, thus limiting the widespread use of PMQ. Although the World Health Organization recommends a single low dose (0.25 mg / kg) to prevent malaria transmission while reducing the risk of hemolysis, malaria treatment still faces the problem of variable drug sensitivity i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 刘芳邓涛伍盛鋆吴亚兰
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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