A kind of preparation method of translucent conductive collagen film
A technology of collagen and collagen film, which is applied in the field of medical biomaterials and tissue engineering, can solve problems such as the preparation method of conductive collagen film that has not been reported, and achieve the effect of simple and stable preparation process, simple operation and low price
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Embodiment 1
[0032] Collagen membrane preparation
[0033] In this example, self-extracted pig's trotter tendon collagen was used. The collagen extraction process was as follows: pig's trotters were purchased from the market, washed with clean water, and the fur and muscles were removed. Use scissors to dissect and separate the white tendon in the trotter, remove the muscle, fat, grease and superficial fascia, rinse with sterile deionized water, and obtain the white tendon ( figure 1 ). The tendon tissue was disinfected with alcohol for 3-5 minutes, rinsed with sterile deionized water for more than 3 times, and the tendon was cut into 3-5 mm microtissues with sharp scissors ( figure 2 ), add sufficient deionized water to rinse for 3-5 times, use sterile filter paper to absorb the indicated oil droplets, and wash until the oil is completely removed. Prepare a 0.2% aqueous solution of glacial acetic acid by volume, filter and sterilize at 4 degrees for later use. Add the shredded tendon ...
Embodiment 2
[0036] Preparation of Conductive Collagen Films
[0037] In this embodiment, silver nanowires are mainly used as the conductive material. Simply ultrasonicate the nano-silver wire aqueous solution for 15 seconds to disperse the silver wire evenly. 0.8 mg / ml collagen solution (same as Example 1) was used to prepare collagen solutions containing silver nanowires at 0.2, 0.4, 0.8, 1.2, 1.6, and 2.0 mg / ml respectively. 1 milliliter of the above-mentioned silver nanowire collagen solution was added to the above-mentioned collagen film sealed with the quartz ring, and air-dried at 40 degrees ( figure 2 C); In order to promote uniform coating of silver nanowires, this step was repeated 3 times. The transparency of nano-silver wire collagen film is shown in the figure ( figure 2 D). Due to the edge effect, the concentration of silver nanowires around the collagen membrane was higher than that in the center. The surface morphology and electrical conductivity of collagen and silv...
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