3D printed bone tissue engineering scaffold with slow-release antibacterial function and preparation method

A technology of bone tissue engineering and 3D printing, applied in additive processing, prosthesis, medical science, etc., can solve the problems of large side effects, poor drug absorption effect, weakened differentiation ability, etc., and achieve good biological activity and good bone repair effect , good biocompatibility

Active Publication Date: 2021-11-02
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, berberine can inhibit the expression of lipid metabolism-related genes in bone marrow mesenchymal stem cells (MSC), weaken the ability to differentiate into adipocytes, and promote the expression of MSC osteogenesis-related genes and the synthesis and secretion of osteocalcin, and increase the level of alkaline phosphate. Enzyme activity enhances the ability of MSCs to differentiate into osteoblasts. Therefore, berberine has the function of promoting osteogenesis. It is often used to treat osteoporosis in clinical practice. However, it is usually taken orally or infused in clinical use. The drug absorption effect is poor and the side effects are large.

Method used

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  • 3D printed bone tissue engineering scaffold with slow-release antibacterial function and preparation method
  • 3D printed bone tissue engineering scaffold with slow-release antibacterial function and preparation method

Examples

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preparation example Construction

[0037] A method for preparing a 3D printing bone tissue engineering scaffold material with a slow-release antibacterial function, comprising the following steps:

[0038] a) dissolving sodium alginate in deionized water to prepare a sodium alginate solution with a mass fraction of 6%;

[0039] b) dissolving berberine in dimethyl sulfoxide to obtain a 0.1 g / ml berberine-dimethyl sulfoxide solution;

[0040] c) According to the ratio of berberine to sodium alginate mass ratio of 0.02~0.2:6, the berberine-dimethyl sulfoxide solution obtained in step b) is added dropwise to the 6% sodium alginate solution obtained in step a), so that The two are mixed evenly to obtain a mixed solution;

[0041] d) Put the mixed solution obtained in step c) at 50-60°C for 30-60 minutes, and then slowly add calcium phosphate to the sodium alginate after the heat preservation according to the mass ratio of calcium phosphate and sodium alginate of 10:3 and berberine mixed solution, uniformly dispers...

Embodiment 1

[0053] In a water bath at 30°C, add sodium alginate powder material to deionized water to prepare a 6% sodium alginate solution, add 1ml of berberine-dimethyl sulfoxide solution with a concentration of 0.1g / ml, and use a magnetic stirrer Mix it well until smooth. After preheating at 50°C for 30 to 60 minutes, the viscosity of the sodium alginate solution is reduced, and then the solid hydroxyapatite powder is slowly added to the preheated solution, and the slurry is mixed evenly with a homogeneous stirrer until no observation is observed. When the agglomeration of hydroxyapatite occurs, the mass ratio of hydroxyapatite, sodium alginate, berberine and deionized water in the final slurry is 20:6:0.1:100, and this mixed slurry is used as 3D printing ink Materials, prepare 30% calcium chloride solution as cross-linking agent. Using 3D inkjet printing technology, the mixed ink is printed into a calcium phosphate scaffold with a first-level macroporous structure, and then the scaff...

Embodiment 2

[0055] According to the method of Example 1, first prepare 3D printing ink, carry out 3D printing of first-level macro-scale macroporous design to prepare scaffolds, then immerse in calcium chloride solution for cross-linking, and then perform freeze-drying treatment, the remaining parameters selection and preparation process and implementation The same as Example 1, the difference is that in this example, the 3D printing ink used in Example 1, the mass ratio of hydroxyapatite, sodium alginate, berberine and deionized water is adjusted to 20: 6: 0.2: 100, made into slurry as 3D printing ink; 30% calcium chloride solution as cross-linking agent. All the other processing methods are the same as those described in Example 1. Compared with Example 1, because the solid content of berberine in the printing slurry is more in this example, the total amount of drug-loaded bone tissue engineering scaffold with slow-release antibacterial function is more, so the scaffold obtained in this...

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Abstract

The invention discloses a 3D printing bone tissue engineering scaffold material with slow-release antibacterial function and a preparation method thereof, which is formed by in-situ cross-linking of a porous calcium phosphate material and sodium alginate, and simultaneously loaded with berberine. The bone tissue engineering scaffold material is mixed with berberine in the printing ink, so that the scaffold has both antibacterial and osteogenic functions. Finally, the pore structure of the scaffold is regulated by 3D printing and post-processing, and the calcium chloride cross-linking agent is changed. Concentration and cross-linking time control the cross-linking degree of the stent, and by changing the drug concentration to regulate the drug loading of the stent to achieve the drug sustained release effect of the stent.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, specifically a 3D printed bone tissue engineering scaffold material with slow-release antibacterial function and a preparation method thereof Background technique [0002] Tissue engineering scaffold is a basic element in tissue engineering. It is generally prepared by using bioactive materials. It can mimic biological functions such as the composition, structure and mechanical properties of human tissue, and is suitable for the growth and reproduction of seed cells. Therefore, tissue engineering Stent is one of the key factors affecting the successful reconstruction of human tissue. The human skeleton is the supporting part of the body and has important physiological functions. Bone tissue has self-healing ability for small local damage, but in the case of large-scale bone defect, bone lesions and trauma cannot repair itself, so bone tissue engineering scaffolds are needed as a sub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/20A61L27/54A61L27/56B33Y70/10B33Y10/00
CPCA61L27/12A61L27/20A61L27/54A61L27/56A61L2300/216A61L2300/404A61L2300/602B33Y10/00B33Y70/00C08L5/04
Inventor 周长春梁洁孙桓樊渝江孙勇吴丽娜胡诚孙建勋张兴栋
Owner SICHUAN UNIV
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