Biological alcoholization method for tobacco leaves

A bio-alcohol and tobacco leaf technology, applied in tobacco, tobacco processing, food science, etc., can solve the problems affecting the quality of deep-processed tobacco leaves, tobacco leaves are prone to insects and mildew, and tobacco leaves are easy to turn brown, achieving excellent stability and Reproducible, low price, clear aroma enhancing effect

Inactive Publication Date: 2019-07-09
南宁雄晋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of natural aging effect is better, but there are also disadvantages: tobacco leaves are rich in nutrients, long-term close contact with the external environment and seasonal changes, tobacco leaves are prone to insects and mildew, and the color of tobacco leaves is easy to brown and darken. Sometimes it affects the quality of tobacco leaf deep-processing products; the second is to add tobacco alcoholizing additives to tobacco leaves
But this method is not suitable for modern cigarette industry production

Method used

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  • Biological alcoholization method for tobacco leaves
  • Biological alcoholization method for tobacco leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Get Aspergillus melleus according to 20% by mass percentage (Aspergillusmelleus) and Aspergillus oryzae (Aspergillus oryzae) according to 80% by mass percentage and mix evenly, and the inoculation amount of mixed strain is inoculated on the seed medium after sterilization by mass percentage 1% in 40°C for static cultivation for 20 hours, and the seed solution was obtained after cultivation;

[0044] Among them, the number of Aspergillus melleus cells is 1 billion / g, and the number of Aspergillus oryzae cells is 10 billion / g;

[0045] Among them, the ingredients of the seed medium are: fructose 80g / L, ammonium tartrate 10g / L, Tween-80 15g / L, concentrated corn steep liquor 10g / L, potassium dihydrogen phosphate 1g / L, copper sulfate 1g / L, manganese sulfate 0.5g / L;

[0046] (2) Seed liquid is inoculated in fermentation medium by the inoculum amount of 20% by mass percentage, cultivates 70h at 20 ℃, obtains mature fermentation liquid, stirring speed 50rpm in the fermenta...

Embodiment 2

[0053] (1) Get Aspergillus melleus according to 30% by mass percentage (Aspergillusmelleus) and Aspergillus oryzae (Aspergillus oryzae) according to 70% by mass percentage and mix evenly, and the inoculation amount of mixed strain is inoculated on the seed medium after sterilization by mass percentage 1% in 40°C for static cultivation for 20 hours, and the seed solution was obtained after cultivation;

[0054] Among them, the number of Aspergillus melleus cells is 3 billion / g, and the number of Aspergillus oryzae cells is 5 billion / g;

[0055] Among them, the ingredients of the seed medium are: fructose 60g / L, ammonium tartrate 15g / L, Tween-80 10g / L, concentrated corn steep liquor 10g / L, potassium dihydrogen phosphate 1g / L, copper sulfate 1g / L, manganese sulfate 0.2g / L;

[0056] (2) Seed liquid is inoculated in fermentation medium by the inoculum amount of 20% by mass percentage, cultivates 70h at 20 ℃, obtains mature fermentation liquid, stirring speed 50rpm in the fermentat...

Embodiment 3

[0063] (1) Get Aspergillus melleus according to 40% by mass percentage (Aspergillusmelleus) and Aspergillus oryzae (Aspergillus oryzae) according to 60% by mass percentage and mix evenly, and the inoculation amount of mixed strain is inoculated on the seed medium after sterilization by mass percentage 3% in 30°C for static cultivation for 30 hours, and the seed solution was obtained after cultivation;

[0064] Among them, the number of Aspergillus melleus cells is 2 billion / g, and the number of Aspergillus oryzae cells is 8 billion / g;

[0065] Among them, the ingredients of the seed medium are: fructose 70g / L, ammonium tartrate 13g / L, Tween-80 13g / L, concentrated corn steep liquor 15g / L, potassium dihydrogen phosphate 2g / L, copper sulfate 1g / L, manganese sulfate 0.5g / L;

[0066] (2) Seed liquid is inoculated in the fermented medium with the inoculum amount of 10% by mass percentage, cultivates 50h at 25 ℃, obtains mature fermented liquid, stirring speed 20rpm in the fermentat...

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Abstract

The invention provides a biological alcoholization method for tobacco leaves. Strains which facilitate acceleration of alcoholization are cultured and fermented, fermentation liquor is subjected to plate-frame filtration and vacuum concentration and then prepared into spray-coating liquid by adding a protection agent, and the spray-coating liquid is uniformly sprayed to the surfaces of the tobaccoleaves. According to the biological alcoholization method for the tobacco leaves, the mixed strains are used for seed culture, afterwards, fermentation is conducted, lignin, starch and proteins in tobacco stem extracts are used as inducing substrates of enzymes, generated laccase is highly targeted to degradation of the lignin in tobacco stems, the degradation efficiency is high, and the contentof the lignin in the tobacco stems is reduced; the starch, the proteins, pectins, hemicellulose and other impurities which affect the smoking taste in the tobacco stems generate the corresponding enzymes through the inducing substrates in the tobacco stem extracts, after the spray-coating liquid is sprayed to the tobacco leaves, the corresponding substrates are degraded, the time for the natural alcoholization process is shortened, and the taste is better and more mellow.

Description

technical field [0001] The invention relates to a tobacco processing technology, in particular to a method for bioalcoholization of tobacco leaves. Background technique [0002] Tobacco leaves are stored under suitable environmental conditions for a certain period of time, and the color, aroma, taste, etc. will be significantly improved. This improvement and change in quality is also called "natural aging". At present, the natural aging technology is mainly divided into three categories: one is the traditional natural aging method, the tobacco leaves are stacked in the warehouse according to certain requirements, and the aging time is 1 to 3 years. This method of natural aging effect is better, but there are also disadvantages: tobacco leaves are rich in nutrients, long-term close contact with the external environment and seasonal changes, tobacco leaves are prone to insects and mildew, and the color of tobacco leaves is easy to brown and darken. Sometimes it affects the qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
CPCA24B15/20
Inventor 庄冉玉小金陈绪高郝志军周广军
Owner 南宁雄晋生物科技有限公司
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