Biological alcoholization method for tobacco leaves
A bio-alcohol and tobacco leaf technology, applied in tobacco, tobacco processing, food science, etc., can solve the problems affecting the quality of deep-processed tobacco leaves, tobacco leaves are prone to insects and mildew, and tobacco leaves are easy to turn brown, achieving excellent stability and Reproducible, low price, clear aroma enhancing effect
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Embodiment 1
[0043] (1) Get Aspergillus melleus according to 20% by mass percentage (Aspergillusmelleus) and Aspergillus oryzae (Aspergillus oryzae) according to 80% by mass percentage and mix evenly, and the inoculation amount of mixed strain is inoculated on the seed medium after sterilization by mass percentage 1% in 40°C for static cultivation for 20 hours, and the seed solution was obtained after cultivation;
[0044] Among them, the number of Aspergillus melleus cells is 1 billion / g, and the number of Aspergillus oryzae cells is 10 billion / g;
[0045] Among them, the ingredients of the seed medium are: fructose 80g / L, ammonium tartrate 10g / L, Tween-80 15g / L, concentrated corn steep liquor 10g / L, potassium dihydrogen phosphate 1g / L, copper sulfate 1g / L, manganese sulfate 0.5g / L;
[0046] (2) Seed liquid is inoculated in fermentation medium by the inoculum amount of 20% by mass percentage, cultivates 70h at 20 ℃, obtains mature fermentation liquid, stirring speed 50rpm in the fermenta...
Embodiment 2
[0053] (1) Get Aspergillus melleus according to 30% by mass percentage (Aspergillusmelleus) and Aspergillus oryzae (Aspergillus oryzae) according to 70% by mass percentage and mix evenly, and the inoculation amount of mixed strain is inoculated on the seed medium after sterilization by mass percentage 1% in 40°C for static cultivation for 20 hours, and the seed solution was obtained after cultivation;
[0054] Among them, the number of Aspergillus melleus cells is 3 billion / g, and the number of Aspergillus oryzae cells is 5 billion / g;
[0055] Among them, the ingredients of the seed medium are: fructose 60g / L, ammonium tartrate 15g / L, Tween-80 10g / L, concentrated corn steep liquor 10g / L, potassium dihydrogen phosphate 1g / L, copper sulfate 1g / L, manganese sulfate 0.2g / L;
[0056] (2) Seed liquid is inoculated in fermentation medium by the inoculum amount of 20% by mass percentage, cultivates 70h at 20 ℃, obtains mature fermentation liquid, stirring speed 50rpm in the fermentat...
Embodiment 3
[0063] (1) Get Aspergillus melleus according to 40% by mass percentage (Aspergillusmelleus) and Aspergillus oryzae (Aspergillus oryzae) according to 60% by mass percentage and mix evenly, and the inoculation amount of mixed strain is inoculated on the seed medium after sterilization by mass percentage 3% in 30°C for static cultivation for 30 hours, and the seed solution was obtained after cultivation;
[0064] Among them, the number of Aspergillus melleus cells is 2 billion / g, and the number of Aspergillus oryzae cells is 8 billion / g;
[0065] Among them, the ingredients of the seed medium are: fructose 70g / L, ammonium tartrate 13g / L, Tween-80 13g / L, concentrated corn steep liquor 15g / L, potassium dihydrogen phosphate 2g / L, copper sulfate 1g / L, manganese sulfate 0.5g / L;
[0066] (2) Seed liquid is inoculated in the fermented medium with the inoculum amount of 10% by mass percentage, cultivates 50h at 25 ℃, obtains mature fermented liquid, stirring speed 20rpm in the fermentat...
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