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Preparation method for coagulase test paper

A technology for detecting test strips and coagulation enzymes, applied in measuring devices, material analysis through observation of the influence of chemical indicators, instruments, etc., can solve the problems of complex AV scoring and discomfort in clinical routine inspections, and achieve easy operation and reduce errors , to ensure the effect of accuracy

Inactive Publication Date: 2019-07-09
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Among them, the AV score is a necessary index, but the AV score is very complicated. It needs to comprehensively integrate the indicators such as the grade of lactobacilli in vaginal secretions, the number of white blood cells, the proportion of toxic white blood cells, and the number of background colonies. This method is not suitable for routine clinical testing, especially Is the use of the hospital outpatient service, etc.

Method used

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  • Preparation method for coagulase test paper
  • Preparation method for coagulase test paper
  • Preparation method for coagulase test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0095] Implementation case 1: Preparation of coagulase test paper

[0096] Dip: 300mM Tris-HCl buffer;

[0097] 5.0mg / mL glycyl-arginine-4-methoxy-β-naphthylamine hydrochloride;

[0098] 3.0mg / mL trehalose;

[0099] 3.0mg / mL PEG-6000;

[0100] pH value is 8.5;

[0101] Filter paper type: 31ET filter paper

[0102] Matching color developing solution: 1.5mg / mL dimethylaminocinnamaldehyde;

[0103] 2.0mg / mL Triton X-100;

[0104] 4.0mg / mL PVP-K90;

[0105] 1000mL distilled water;

[0106] Automatic drying method of coagulation enzyme test paper impregnation dryer:

[0107] After starting up, set the equipment temperature to 80°C for the main heating and 60°C for the auxiliary heating. When the heating temperature of the host reaches 80°C, the immersion starts when the temperature of each drying chamber reaches 60°C;

[0108] Impregnation dryer speed setting: 10.0Hz.

Embodiment example 2

[0109] Implementation case 2: Preparation of coagulase test paper

[0110] Dip: 300mM Tris-HCl buffer;

[0111] 5.0mg / mL Glycyl-Arginine-β-Naphthylamine;

[0112] 3.0mg / mL trehalose;

[0113] 3.0mg / mL PEG-6000;

[0114] pH value is 8.5;

[0115] Filter paper type: 31ET type filter paper

[0116] Matching color developing solution: 1.5mg / mL dimethylaminocinnamaldehyde;

[0117] 2.0mg / mL Triton X-100;

[0118] 4.0mg / mL PVP-K90;

[0119] 1000mL distilled water;

[0120] Automatic drying method of coagulation enzyme test paper impregnation dryer:

[0121] After starting up, set the equipment temperature to 80°C for the main heating and 60°C for the auxiliary heating. When the heating temperature of the host reaches 80°C, the immersion starts when the temperature of each drying chamber reaches 60°C;

[0122] Impregnation dryer speed setting: 10.0Hz.

Embodiment example 3

[0123] Implementation case 3: Preparation of coagulase test paper

[0124] Dip: 300mM Tris-HCl buffer;

[0125] 5.0mg / mL glycyl-arginine-7-amino-4-methylcoumarin;

[0126] 3.0mg / mL trehalose;

[0127] 3.0mg / mL PEG-6000;

[0128] pH value is 8.5;

[0129] Filter paper type: 31ET type filter paper

[0130] Matching color developing solution: 1.5mg / mL dimethylaminocinnamaldehyde;

[0131] 2.0mg / mL Triton X-100;

[0132] 4.0mg / mL PVP-K90;

[0133] 1000mL distilled water;

[0134] Automatic drying method of coagulation enzyme test paper impregnation dryer:

[0135] After starting up, set the equipment temperature to 80°C for the main heating and 60°C for the auxiliary heating. When the heating temperature of the host reaches 80°C, the immersion starts when the temperature of each drying chamber reaches 60°C;

[0136] Impregnation dryer speed setting: 10.0Hz.

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Abstract

The invention relates to a preparation method for coagulase test paper. According to employed impregnation liquid, through utilization of a buffer system, a good neutral-weak alkaline reaction environment for reaction is provided; through independent or combined use of dispersing agents, dispersing capability of the test paper can be improved, and uniform distribution of colors is facilitated; andthrough utilization of a stabilizer, enzyme substances in a sample can be protected, and detection accuracy is improved. According to employed color development liquid, through utilization of the stabilizer and the dispersing agents, a good reaction environment for improvement of uniformity and stability of the test paper is provided. According to the method, the test paper is prepared through adoption of an impregnation drier automatic drying method. An impregnation drying mode has the advantages of convenience, saving time and automation. Influence resulting from artificial errors in a testpaper preparation process is effectively controlled. According to an impregnation drier, through main and auxiliary heating, a temperature is monitored comprehensively, the temperature is set in a stated range, errors between test paper batches can be greatly reduced, and test paper preparation accuracy, stability and uniformity are ensured.

Description

Technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, and specifically relates to a preparation method of a coagulase detection test paper. Background technique [0002] Bacterial vaginosis (BV) is a clinical syndrome without vaginal mucosal inflammation caused by a variety of bacteria, and the number of clinical cases is much higher than that of trichomonal vaginitis and fungal vaginitis. Because BV is related to obstetrical and various gynecological diseases such as premature birth, chorioamnionitis, postpartum endometritis, etc., it has received more and more attention from clinicians and scholars. [0003] In recent years, studies have found that there are two major types of bacteria that cause vaginal bacterial infections. One is caused by anaerobic bacteria and facultative anaerobes, which are clinically called bacterial vaginosis (BV); the other is caused by aerobic bacteria. This type of bacteria is clinically called aerobic v...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 朱子是崔秀丽孙德亚林月何浩会孙成艳
Owner DIRUI MEDICAL TECH CO LTD
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