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Preparation method of gold nanocluster fluorescent probe and test paper based on zein

A technology of zein and gold nanoclusters is applied in the field of fluorescent probes to achieve the effects of easy mass extraction, simple operation and rich sources

Active Publication Date: 2021-09-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, zein has been used in the preparation of semiconductor CdS quantum dots and the synthesis of 30-80nm gold and silver nanoparticles under the action of an external reducing agent, but the synthesis method and related applications of gold nanoclusters based on zein have not yet been developed. see the report

Method used

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  • Preparation method of gold nanocluster fluorescent probe and test paper based on zein
  • Preparation method of gold nanocluster fluorescent probe and test paper based on zein
  • Preparation method of gold nanocluster fluorescent probe and test paper based on zein

Examples

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Effect test

Embodiment 1

[0027] Example 1: Preparation and purification of zein-gold nanoclusters

[0028] Add 50mg of zein into 5mL of 1M sodium hydroxide solution, heat to dissolve, and mix with an equal volume of chloroauric acid aqueous solution (25mM). The molar ratio of zein to chloroauric acid is 1:18. Under stirring conditions, react at 80°C for 10 minutes to obtain a gold nanocluster solution with zein as a ligand, which is mixed with 10 mL of 0.2 mol / L phosphate buffer solution, and the zein-gold nanoclusters form a precipitate. The fully reacted chloroauric acid etc. still remain in the solution. The mixture was centrifuged at 12000 rpm for 20 minutes, the supernatant was discarded, and the precipitate was washed with phosphate buffer solution to obtain purified zein-gold nanoclusters. The obtained zein-gold nanoclusters were redispersed in deionized water to obtain zein-based gold nanocluster fluorescent probes. The fluorescence spectrum was measured by a fluorescence spectrometer, and t...

Embodiment 2

[0029] Embodiment 2: Preparation of zein-gold nanoclusters

[0030] Add 25mg of zein into 5mL of 0.7M sodium hydroxide solution, heat to dissolve, and mix with an equal volume of chloroauric acid aqueous solution (14mM). The molar ratio of zein to chloroauric acid is 1:20. Under the condition of vigorous stirring, react at 60° C. for 5 minutes to obtain a gold nanocluster solution with zein as a ligand.

Embodiment 3

[0031] Example 3: Preparation of Zein-Gold Nanoclusters

[0032] Add 17.5mg of zein into 5mL of 0.5M sodium hydroxide solution, heat to dissolve, and mix with an equal volume of chloroauric acid aqueous solution (1mM), the molar ratio of zein to chloroauric acid is 1:2, Under the condition of vigorous stirring, react at 40° C. for 12 minutes to obtain a gold nanocluster solution with zein as a ligand.

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Abstract

The preparation method of the zein-based gold nano-cluster fluorescent probe and test paper of the present invention belongs to the technical field of fluorescent probes, and comprises the following steps: under vigorous stirring, the zein solution is mixed with the chloroauric acid solution, heated React to obtain the crude product solution of gold nanoclusters; mix with acidic buffer solution, centrifuge and wash to obtain purified gold nanoclusters; redisperse gold nanoclusters in deionized water to obtain zein-based gold nanocluster fluorescence Probes; or redisperse gold nanoclusters in ethanol solution, add glycerin or polyethylene glycol, take the mixed solution and drop it on a polytetrafluoroethylene gasket, and obtain zein-based gold nanocluster fluorescent test paper after drying . The raw materials used in the invention are rich in sources and low in price, the preparation method is simple to operate, and the synthesized product has two-color fluorescence properties, and can realize the ratio fluorescence detection of silver ions and mercury ions.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probes, and mainly relates to a method for synthesizing and purifying a zein-based gold nanocluster fluorescent probe, which can be used for detecting silver ions and mercury ions by a fluorescence ratio method. Background technique [0002] Gold nanoclusters are new nanomaterials composed of ligands and several to dozens of gold atoms / ions. Because of its ultra-small size (about 2nm), excellent fluorescence properties and good biocompatibility, gold nanoclusters have shown great application potential in biochemical analysis, biological imaging, lighting materials, etc. At present, gold nanoclusters are mostly synthesized using biomolecules such as proteins, small peptides, amino acids, and nucleic acids as ligands. However, commonly used biomolecular ligands, such as glutathione, serum albumin, lysozyme, insulin, etc., are expensive, which makes the preparation cost of gold nanoclusters high...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6421G01N2021/6432
Inventor 黄臻臻段博惠李月王敏
Owner JILIN UNIV