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A kind of detection kit for the determination of creatinine by enzymatic method and using method thereof

A technology for detection kits and kits, applied in measuring devices, color/spectral characteristic measurement, and analysis through chemical reactions of materials, etc., can solve problems such as interference of colorimetric detection methods, interference of anti-oxidative reaction systems, etc. , to achieve good solubilization effect, avoid interference of metal ions, and low cost

Active Publication Date: 2021-10-12
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bilirubin not only interferes with the color contrast detection method due to its color characteristics, but also interferes with the reaction system indicated by the Tinder reaction because of its antioxidant properties

Method used

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  • A kind of detection kit for the determination of creatinine by enzymatic method and using method thereof
  • A kind of detection kit for the determination of creatinine by enzymatic method and using method thereof
  • A kind of detection kit for the determination of creatinine by enzymatic method and using method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation of sarcosine oxidase

[0053] Step 1. Obtain the target sequence

[0054]Using Bacillus CGMCC No.17306 as the starting strain, the whole genome was detected and compared with the existing sarcosine oxidase gene to obtain the original sarcosine oxidase gene, such as Figure 4 As shown, the gene sequence coding for sarcosine oxidase derived from microorganisms is modified and fully synthesized, as shown in SEQ No.1, and the amino acid sequence encoded by sarcosine oxidase is shown in SEQ No.2.

[0055] The preservation information of the starting strain is: a marine bacterium LYH18 strain, Bacillus sp., which has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on March 6, 2019, address: Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Courtyard 1, Beichen West Road, Chaoyang District, City, Zip Code: 100101, and its biological deposit number is CGMCC No.17306.

[0056] St...

Embodiment 2

[0077] A detection kit for the enzymatic determination of creatinine in this embodiment, the specific raw materials and proportioning are as follows:

[0078] The reagent group H1 comprises:

[0079]

[0080] The reagent group H2 comprises:

[0081]

Embodiment 3

[0083] A detection kit for the enzymatic determination of creatinine in this embodiment, the specific raw materials and proportioning are as follows:

[0084] The reagent group H1 comprises:

[0085]

[0086] The reagent group H2 comprises:

[0087]

[0088]

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Abstract

The invention relates to the field of biochemical detection, in particular to a detection kit for enzymatic determination of creatinine and a use method thereof. The kit includes various reagents such as sarcosine oxidase, creatinase, catalase and buffer prepared by using the genetically engineered bacteria GLYH18 prepared by the LYH18 strain with the preservation number CGMCC No.17306. The reagents are mixed, and the final reactant is placed under a biochemical analyzer to detect the change of absorbance to calculate the content of creatinine. The preparation method of the sarcosine oxidase in the kit is simple and low in cost. The kit added with this enzyme can quickly and accurately determine the content of creatinine in the sample, and the kit can remain stable at room temperature, which is more conducive to practice It is also more conducive to industrial production.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a detection kit for enzymatic determination of creatinine and a use method thereof. Background technique [0002] The etiology of kidney disease is complex. Since the pathogenesis of most "primary" glomerular diseases is not fully understood, the current treatment of glomerular diseases is mostly empirical treatment, and there is a lack of etiological treatment. In the field of glomerular disease treatment, prospective, randomized controlled clinical treatment trials are very limited. Nevertheless, looking back over the past three decades, some landmark treatments have been used clinically at each stage, and have improved the treatment level of kidney diseases to a certain extent. [0003] The main clinical manifestations of kidney disease are proteinuria, hematuria, edema, hypertension, renal insufficiency, etc. Over the years, clinicians have conducted comprehensive analy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78G01N2021/775
Inventor 迟乃玉刘洋张庆芳于爽希伦
Owner DALIAN UNIV
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