Method for performing living tracing on exogenous cells by using magnetosome coding gene

Abstract

A method for performing living tracing on exogenous cells by using a magnetosome coding gene includes importing a key gene mms6 encoding the magnetosome into an exogenous cell genome and inducing theexogenous cells to express the mms6 protein which can bind endogenous iron ions to form endogenous ferromagnetic nanoparticles in vivo; after the exogenous cells are transplanted into the body, performing nuclear magnetic resonance scanning periodically to detect endogenous ferromagnetic nanoparticle signals by nuclear magnetic resonance T2 relaxation images. The location of the exogenous cells transplanted can be positioned timely. The tracer signal generated by the magnetosome encoding gene mms6 can be passed on to daughter cells, so that the exogenous cell tracer signal persists, and the long-term living tracing of the exogenous cells can be achieved.

Description

technical field [0001] The invention belongs to the field of exogenous cell tracing, and in particular relates to a method for in vivo tracing of exogenous cells by using magnetosome coding genes. Background technique [0002] At present, stem cell replacement therapy has become a very promising treatment method. In both basic research and clinical research, stem cell therapy has shown relatively superior therapeutic effects. [0003] In recent years, my country has vigorously supported stem cell clinical research, and gradually put stem cell therapy on the agenda. This makes stem cell therapy a very marketable treatment. At present, neural stem cells can be used to treat Parkinson's disease, cerebral palsy, stroke, traumatic brain injury, multiple sclerosis, spinal cord injury and other diseases. [0004] It is very critical to evaluate the therapeutic effect of stem cells, which is an important factor in determining whether a stem cell therapy can be carried out clinical...

AI Technical Summary

Problems solved by technology

[0006] For more than 20 years, stem cell tracing has mainly focused on the development of new tracer materials. However, these materials have a drawback. Take ferroferromagnetic nanoparticles as an example. In the initial stage of transplantation, although the tracer signal can be detected by nuclear Resonance and other imaging equipment, but because the tracer material is provided exogenously, it will not be passed on indefinitely with the proliferation of cells. In fact, the tracer signal will be continuously diluted and excreted with metabolism. In vitro, so that long-term tracing cannot be achieved, and the fundamental problem of tracing cannot be solved

Although this method can achieve long-term stem cell tracking, at present, the imaging recognition signals generated by most imaging reporter genes need special equipment, and some cells with particularly poor penetration and difficult to be identified by current clinical imaging equipment Stem cells, unable to trace exogenous cells in vivo

[0008] In addition to the live tracking of stem cells, the living tracking of other cells also faces the same problem, such as the use of T cells in tumor cell therapy; DC cells or CIK cells used in immunotherapy, etc. Although these exogenously provided cells can The treatment effect is evaluated, but it is difficult to accurately judge the state and location distribution of cells

Images