Hybrid peptide with functions of regulating immunity, resisting oxidation and inflammation and detoxifying body, and preparation method and application thereof

Abstract

The invention relates to a hybrid peptide with functions of regulating immunity, resisting oxidation and inflammation and detoxifying the body, and a preparation method and application thereof. The hybrid peptide named LTP is obtained through design by a protein engineering computer, heterozygosis, optimization, and in vitro and in vivo rescreening of antibacterial peptide LL-37 and thymopentin TP5, wherein the amino acid sequence is represented by SEQ ID NO.1. The hybrid peptide LTP has high bidirectional immunomodulatory activity, can enhance the immune function of the body under a normal orimmunosuppressed state, and can protect the body from injuries caused by immunosuppression; in an inflammatory state, the LTP can neutralize and eliminate endotoxin, inhibit oxidation reaction and inflammatory reaction of the body and alleviate injuries to tissues by the inflammatory reaction, has the advantages of low cytotoxicity, high safety, simplicity in preparation, low cost and the like, and can be used as an ideal immune, anti-oxidative and anti-inflammatory regulator or an immune anti-oxidative detoxification peptide, thereby having high application potential and value.

Description

technical field [0001] The invention relates to the field of genetic engineering and biological preparations, in particular to an immune, anti-oxidation and detoxification hybrid peptide with functions of immune regulation, anti-oxidation, anti-inflammation and digestion, and its preparation method and application. Background technique [0002] When animals or humans are young, weak, disease, stress, pathogenic infection, etc., their immunity is often reduced, and then secondary infection (bacterial or viral mixed infection) occurs, causing inflammatory reactions (red, hot, swollen) , pain, etc.). At present, for the treatment of infection and inflammation, the traditional and commonly used prevention and treatment strategy is to use antibiotics and hormone anti-inflammatory drugs (such as hydrocortisone, dexamethasone, etc.), although it can effectively control infectious inflammation and non-infectious inflammation, However, continuous use can cause various side effects: ...

AI Technical Summary

Problems solved by technology

There are obvious problems in the use of antibiotics or hormonal anti-inflammatory drugs in the treatment of infection and inflammation: although antibiotics can reduce or kill pathogenic bacteria, they cannot improve the immune function of the body. On the contrary, the bacteria killed by antibiotics may also produce endotoxin or Exotoxins, which in turn aggravate the inflammatory response and even lead to systemic inflammatory response syndrome (Botwinski, 2001), which can cause fever, anorexia, excessive energy consumption in the body, decomposition of body tissues, and decline in immunity and production performance in animals. Avian mortality (Botwinski, 2001; Zinner, 1999)

Therefore, in the field of traditional medicine, the clear separation of anti-inflammatory drugs and immune-enhancing drugs does not meet the actual needs of the body or the interaction between anti-inflammatory and immune, and greatly increases the complexity and antagonism of clinical drug selection.

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