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Preparation containing camellia oleifera extract and application of preparation

An extract and preparation technology, which is applied in the application field of Camellia oleifera extract to achieve the effect of improving liver fibrosis

Pending Publication Date: 2019-08-23
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More than one million people die from this disease every year, especially for end-stage liver disease, and there is no effective reversal drug

Method used

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  • Preparation containing camellia oleifera extract and application of preparation
  • Preparation containing camellia oleifera extract and application of preparation
  • Preparation containing camellia oleifera extract and application of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Animal experiment scheme and its influence on mouse organ index

[0030] C57BL / 6J mice were obtained from The Jackson Laboratory (JAX, ME, USA). 24 mice were randomly divided into 4 groups.

[0031] Group 1 (normal control group) was treated with olive oil (2 ml / kg, intraperitoneal injection) twice a week (on the first and fourth days of each week) for a total of 6 weeks.

[0032] Group 2 (hepatic fibrosis control group, CCl 4 group) CCl was given by intraperitoneal injection twice a week 4 (olive oil solvent, content is 50%, injection volume 2mL / kg), totally 6 weeks.

[0033] Group 3 (CCl 4 + camellia oil group): first receive CCl in the same way as the second group 4 Two weeks to establish liver damage and fibrosis, and then from week 3 to week 6, daily treatment with camellia oil (2ml / kg, injected into the stomach) while still giving CCl 4 .

[0034] Group 4 (Camellia oleifera oil group): From the first week to the sixth week, they were treated with...

Embodiment 2

[0041] Example 2: Histopathological examination of liver tissue changes

[0042] The liver tissue sample taken out in Example 1 was fixed in 10% paraformaldehyde solution, then embedded in paraffin, and sectioned at 4 μm. Sections were stained with hematoxylin and eosin (H&E) to observe pathological changes. Masson staining and Sirius-Red staining were used to assess changes in fibrosis. Liver fibrosis was observed under a microscope by pathologists. see results Figure 1 ~ Figure 3 . After comparison, the second group (fibrosis control group) showed obvious fatty degeneration, liver cell arrangement disorder, liver cell swelling, necrosis, and inflammatory cell infiltration (see figure 1 -B), fibrous hyperplasia in the portal area, accompanied by obvious fiber bridging, including between the portal canal and between the portal canal and the central vein (see figure 2 -B, image 3 -B). Compared with group 2, group 3 showed significantly less liver damage (see figure 1...

Embodiment 3

[0049] Embodiment three: biochemical analysis

[0050] According to the scheme of Example 1, after the last CCl in mice 4 After intraperitoneal injection, serum samples were collected for the measurement of liver function markers alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Serum laminin, collagen type IV was detected using immunoassay kits to assess liver fibrosis. To assess oxidative stress, the liver homogenate was measured for the levels of SOD (superoxide dismutase), MDA (malondialdehyde, malondialdehyde), GSH (glutathione, glutathione). The detections are all carried out by methods commonly used in the art.

[0051] The results show:

[0052] 1. Camellia oil improves CCl 4 Liver function in mice with induced hepatic fibrosis.

[0053] Test results from aspartate aminotransferase and alanine aminotransferase levels (see Figure 4 -A, B) It can be seen that compared with the normal control group, the enzymatic indexes of the fibrosis control g...

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Abstract

The invention belongs to the field of preparations and provides a preparation containing a camellia oleifera extract and application of the preparation. Camellia oleifera oil is adopted as the camellia oleifera extract. The provided preparation containing the camellia oleifera extract can effectively alleviate liver fibrosis conditions and bring a new approach for the treatment of liver fibrosis.

Description

technical field [0001] The invention belongs to the field of preparations and relates to the application of camellia oleifera extract. Background technique [0002] Liver fibrosis is a part of the pathological development of many chronic liver diseases, such as viral hepatitis, liver damage caused by toxins, alcoholic hepatitis, non-alcoholic hepatitis, etc. As liver fibrosis progresses, it may lead to cirrhosis, portal hypertension, liver decompensation, liver tumors, and even death. More than one million people die from this disease every year, especially for end-stage liver disease, and there is no effective reversal drug yet. Liver transplantation became the most effective method, but the shortage of donors caused many patients to die while waiting. Therefore, the development of drugs targeting liver fibrosis is imminent. Contents of the invention [0003] The inventors of the present application have found through research that Camellia oleifera extract has a signi...

Claims

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Application Information

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IPC IPC(8): A61K36/82A61P1/16A23L33/105
CPCA23V2002/00A61K36/82A23L33/105A61P1/16A23V2200/32A23V2250/21
Inventor 徐迅迪雷晓华
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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