IHNV genetically engineered oral microsphere vaccine and preparation method and application thereof

A microsphere vaccine and genetic engineering technology, applied in the field of genetic engineering, can solve problems such as unexplored outbreaks, and achieve the effects of increasing stability and uniformity, simple process and less time-consuming

Pending Publication Date: 2019-08-23
HEBEI NORMAL UNIV
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Problems solved by technology

[0007] With the rapid development of biotechnology, the research on IHNV has become more comprehensive and in-depth, but an effective way to control the outbreak of the disease has not yet been explored

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  • IHNV genetically engineered oral microsphere vaccine and preparation method and application thereof
  • IHNV genetically engineered oral microsphere vaccine and preparation method and application thereof
  • IHNV genetically engineered oral microsphere vaccine and preparation method and application thereof

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Embodiment Construction

[0038] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0039] 1. a preparation method of IHNV genetic engineering oral microsphere vaccine, comprises the following steps:

[0040] 1.1 Construction of expression vector pET-22b-m

[0041] 1.1.1 IHNVm gene primer design: According to the complete sequence of the IHNVm gene published by GenBank and the sequence of the vector pET-22b, the upstream primer M-F and the downstream primer M-R were designed and synthesized, wherein the sequence ...

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Abstract

The invention provides an IHNV genetic engineering oral microsphere vaccine and a preparation method and application thereof. The vaccine is characterized in that the preparation method comprises thefollowing steps: construction of an expression vector pET-22b-m, expression of M protein in the expression vector pET-22b-m, and preparation of the oral microsphere vaccine. The microsphere vaccine has the advantages that adopted sodium alginate and chitosan are natural polysaccharides, safe and free of toxics and pollution, and can stably increase the stability and homogeneity of target protein,the chitosan itself can inhibit the bacterial activity, and a guarantee is provided for the long-term and stable preservation of the microsphere vaccine. The provided preparation method is simple in process and takes less time, and the success rate of the preparation is high. The provided IHNV genetic engineering oral microsphere vaccine has good release properties and acid resistance, and it is found through immune experiments that the vaccine has a certain immune protection effect on rainbow trout.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and relates to the research on genetic vaccines for animal viral infectious diseases, in particular to an IHNV genetic engineering oral microsphere vaccine and its preparation method and application. Background technique [0002] Infectious hematopoietic necrosis virus (IHNV) is a fish viral pathogen that can cause hematopoietic necrosis (Infectious hematopoietic necrosis) in cold-water fish such as trout (Oncorhunchus mykiss) and salmon (Salmo salar), IHN). IHNV was first discovered in Washington and Oregon sockeye salmon (Oncorhynchus nerka) hatchlings in the 1950s. It is excreted into the water along with the excretion of feces, urine, and gonad products of virus-carrying fish. Then it invades the healthy fish body through the gills and digestive tract, and then realizes the virus infection. The transmission mode of IHNV is generally horizontal transmission, but there are also ver...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/205A61K9/50A61K47/36A61P31/14C12N15/66C12N15/70C07K14/145
CPCA61K39/12A61K9/5036A61P31/14C12N15/66C12N15/70C07K14/005A61K2039/542C12N2760/20034A61K2039/552C12N2760/20022
Inventor 赵宝华潘璇吕春爽陈令孝安红艳田晟源
Owner HEBEI NORMAL UNIV
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