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Bacillus HN-2 compound for killing pathogenic bacteria and preparation method of bacillus HN-2 compound

A technology of HN-2 and Bacillus, which is applied in the field of biocontrol bacteria, can solve the problems that the soil cannot achieve the expected effect, it is difficult to maintain the population number, and the activity of Bacillus is decreased, so as to achieve difficult drug resistance, good inhibitory effect and inhibitory effect. good bacterial activity

Pending Publication Date: 2019-08-30
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of application, many problems were found. The activity of Bacillus colonization in the soil decreased, the soil was affected by many factors and could not achieve people's expected results, and it was difficult to maintain the number of populations in a wild environment, etc. A series of problems

Method used

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  • Bacillus HN-2 compound for killing pathogenic bacteria and preparation method of bacillus HN-2 compound
  • Bacillus HN-2 compound for killing pathogenic bacteria and preparation method of bacillus HN-2 compound
  • Bacillus HN-2 compound for killing pathogenic bacteria and preparation method of bacillus HN-2 compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Compounds prepared by n-butanol extraction

[0041] Inoculate the preserved biocontrol strain HN-2 on LB medium for activation and culture at 28°C, cultivate for 2 days, pick an appropriate amount of biocontrol strain HN-2 and add it to the liquid LB medium, shake the table at 28°C, 180 rpm Shake the bacteria for 48 hours per minute to obtain the fermentation broth, use a high-speed centrifuge, and centrifuge at 10,000 rpm for 10 minutes to remove the bacteria, and keep the supernatant to obtain the bacteria-free fermentation broth.

[0042] Add n-butanol at a ratio of 1:1 between the aseptic fermented liquid and n-butanol, let the two solutions fully mix and stand still overnight (12 hours), extract with a separatory funnel to obtain n-butanol in the upper layer, and use a rotary evaporator , evaporate the organic solvent n-butanol to obtain the compound described in this application.

Embodiment 2

[0044] Compounds prepared by hydrochloric acid precipitation

[0045] With reference to the method of Example 1, the aseptic fermented liquid was obtained, titrated with 2mol / L hydrochloric acid, adjusted the pH value of the fermented liquid, and stopped dripping hydrochloric acid when the pH value was adjusted to 2, sealed and put into 4 ℃ of refrigerators to refrigerate overnight (12 hours), and then use a high-speed centrifuge at 4° C., 10,000 rpm, centrifuge for 10 minutes, discard the supernatant, and obtain a precipitate, which is the compound described in this application.

Embodiment 3

[0047] Preparation of Compounds by Ammonium Sulfate Saturated Precipitation

[0048] Refer to the method of Example 1 to obtain sterile fermentation liquid, centrifuge the bacterial liquid at 10,000 rpm for 10 min, slowly add ammonium sulfate powder, precipitate protein, leave it at 4°C overnight (12 hours), and centrifuge to obtain the precipitate, which is the present application said compound.

[0049] For the convenience of storage, the compounds obtained in the above examples were dried with a freeze dryer to obtain dry powders and then stored.

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Abstract

The invention relates to the field of biocontrol bacteria, and discloses a bacillus HN-2 compound for killing pathogenic bacteria and a preparation method of the bacillus HN-2 compound. The compound is extracted from Bacillus velezensis HN-2 with a preservation number of CCTCC NO: M 2018382. Research shows that active ingredients can be directly obtained from fermentation a broth of the bacterialstrain after simple treatment, and the bacillus HN-2 compound has the ability of inhibiting the growth of Xanthomonas Xoc, Xoo and Xcc effectively, and also has good tolerance to strong acid, strong alkali, high temperature and ultraviolet radiation. The bacillus HN-2 compound is better in antibacterial activity than Bismerthiazol, a commonly used medicament in the market, and has the potential tobe developed into a new kind of medicament for the prevention and cure of bacteriosis caused by Xanthomonas.

Description

technical field [0001] The invention relates to the field of biocontrol bacteria, in particular to a bacillus HN-2 compound for killing pathogenic bacteria and a preparation method thereof. Background technique [0002] Bacterial diseases of rice are one of the important diseases in rice production in my country. Taking bacterial blight and streak disease as examples, the two diseases currently occur on an area of ​​more than 80 million mu all year round, causing a loss of 10%-20% of rice yield, and in severe cases, it can reach more than 50% or even extinction. [0003] Chemical control is still an indispensable and important means to prevent and control the disease, but the control effect of chemical agents on the disease has not been ideal, mainly due to the lack of resources of available chemical species and the low activity of one agent. Commonly used pesticides for the prevention and treatment of rice bacterial diseases include: dysonium, nickel methiram, 5-oxphenazin...

Claims

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Application Information

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IPC IPC(8): C12P1/04A01N63/02A01P1/00C12R1/07
CPCC12P1/04C12R2001/07C12N1/205A01N63/10
Inventor 靳鹏飞谭峥缪卫国刘文波王皓楠王雨
Owner HAINAN UNIVERSITY
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