Plant urease catalysis and grass planting combined sand stabilization method

Abstract

The invention relates to a plant urease catalysis and grass planting combined sand stabilization method. Distilled water in which trehalose is added is adopted as a solvent to extract high-activity urease from bean flour; Al2O3 is added to the obtained urease liquid, and then the mixture and a gel solution (composed of urea and calcium acetate with the same molar concentration) are subjected to isovolumetric mixing; grass seeds or tree seeds are added into the mixed solution, and the mixture is sprayed into dry sand; the urea in the gel solution is firstly hydrolyzed into CO<32-> and NH<4+> byurease, the CO<32-> is bonded with Ca<2+> of the calcium acetate to generate calcium carbonate, and a hardened layer is formed on the surface of aeolian sand, so that the water holding capacity of aeolian sand is improved. The grass seeds or the tree seeds are buried in a desert and germinate and survive in a later period, so that the effects of sand stabilization and sand prevention are achieved. According to the method, the plant urease is directly used for catalytic reaction, carbonate precipitation is induced, the calcium carbonate extraction efficiency is high, the environmental requirement is low, and the method is beneficial to application and popularization.

Description

technical field [0001] The invention relates to a sand-fixing method combined with plant urease catalysis and grass planting, which belongs to the plant urease-induced calcium carbonate deposition method, and can be used for dust prevention and sand fixation, and improvement of ecological environment. Background technique [0002] Biodiagenetic and mineralization is a natural phenomenon, which has played an important role in the history of the formation of the earth, which is the result of geochemical research in the past thirty years. It is of great significance to the development of geotechnical engineering technology to re-understand the role and status of biodiagenesis and mineralization. The principle is that urease is produced during the growth and reproduction of microorganisms, which continuously decomposes urea to form CO 3 2- , in the negatively charged aqueous solution at the bacterial cell membrane interface, it continuously chelates Ca in the environment with ...

AI Technical Summary

Problems solved by technology

Some researchers use organic solvents such as ethanol and acetone to extract urease from soybeans, such as "Extraction and Activity Determination of Soybean Urease" by Lin Liyun et al. ("Food Research and Development", 2013, Vol.34, No.9, 85- 88), the published patent CN103601602A "Extraction method and application of urease-inhibiting components in privet leaves", but the activity of soybean urease extracted by ethanol and acetone is low, the operation process is cumbersome, and the acetone solvent is flammable and toxic, which is not controlled Chemicals that are not conducive to the extraction of urease in large quantities on the actual engineering site

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