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Preparation method and application of a kind of 3d-rgo/fe3o4-aunps/hp-β-cd composite material

A technology of fe3o4-aunps and nano-composite materials, which is applied in the field of detection of guanine and adenine, can solve the problem that the detection effect is not very significant, and achieve the effect of excellent electrocatalytic performance, good conductivity and strong catalytic ability

Active Publication Date: 2021-07-02
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, after loading β-CD on the surface of two-dimensional graphene, it not only improves the water solubility and the recognition ability of guest molecules, but also enhances the electrochemical performance, but the detection effect is not very significant.

Method used

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  • Preparation method and application of a kind of 3d-rgo/fe3o4-aunps/hp-β-cd composite material
  • Preparation method and application of a kind of 3d-rgo/fe3o4-aunps/hp-β-cd composite material
  • Preparation method and application of a kind of 3d-rgo/fe3o4-aunps/hp-β-cd composite material

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Experimental program
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Embodiment 1

[0037] A 3D-rGO / Fe 3 o 4 -AuNPs / HP-β-CD nanocomposite preparation method, comprising the following steps:

[0038] 1) Fe 3 o 4 - Preparation of AuNPs nanoparticles:

[0039] First, 50mg of Fe 3 o 4 Add to 50mL ultrapure water, after ultrasonic dispersion, in N 2 Then add 180μLAPTES under the atmosphere, stir mechanically at room temperature for 7h, wash the black precipitate repeatedly with ultrapure water and ethanol, separate it with magnetic hysteresis, and finally dry it in vacuum to obtain aminated Fe 3 o 4 (Fe 3 o 4 -NH 2 ). Then take 20mg Fe 3 o 4 -NH 2 The powder was ultrasonically dispersed in 20 mL of ultrapure water, 40 mg of AuNPs powder was added, stirred at room temperature for 3 h, washed repeatedly and separated by magnetic hysteresis, and dried in vacuum at 50 °C for 8 h to obtain Fe 3 o 4 - AuNPs;

[0040] 2) 3D-rGO / Fe 3 o 4 - Preparation of AuNPs nanomaterials:

[0041] First, graphene oxide (GO) (40mg / 20mL) was ultrasonically crushed for...

Embodiment 2

[0064] A 3D-rGO / Fe 3 o 4 -AuNPs / HP-β-CD nanocomposite preparation method, comprising the following steps:

[0065] 1) Fe 3 o 4 - Preparation of AuNPs nanoparticles:

[0066] First, 51mg of Fe 3 o 4 Added to 60mL ultrapure water, after ultrasonic dispersion, in N 2 Then add 190μLAPTES under the atmosphere, stir mechanically at room temperature for 8h, wash the black precipitate repeatedly with ultrapure water and ethanol, separate it with magnetic hysteresis, and finally dry it in vacuum to obtain aminated Fe 3 o 4 (Fe 3 o 4 -NH 2 ). Then take 21mg Fe 3 o 4 -NH 2 The powder was ultrasonically dispersed in 25 mL of ultrapure water, 41 mg of AuNPs powder was added, stirred at room temperature for 4 h, washed repeatedly and separated by magnetic hysteresis, and vacuum dried at 50 °C for 9 h to obtain Fe 3 o 4 - AuNPs;

[0067] 2) 3D-rGO / Fe 3 o 4 - Preparation of AuNPs nanomaterials:

[0068] First, graphene oxide (GO) (40mg / 20mL) was ultrasonically crushed for ...

Embodiment 3

[0082] A 3D-rGO / Fe 3 o 4 -AuNPs / HP-β-CD nanocomposite preparation method, comprising the following steps:

[0083] 1) Fe 3 o 4 - Preparation of AuNPs nanoparticles:

[0084] First, 49 mg of pre-synthesized Fe 3 o 4 Added to 40mL ultrapure water, after ultrasonic dispersion, in N 2 Then add 170 μL APTES under the atmosphere, stir mechanically at room temperature for 6 hours, wash the black precipitate repeatedly with ultrapure water and ethanol, separate it with magnetic hysteresis, and finally dry it in vacuum to obtain aminated Fe 3 o 4 (Fe 3 o 4 -NH 2 ). Then take 19mg Fe 3 o 4 -NH 2 The powder was ultrasonically dispersed in 15 mL of ultrapure water, 39 mg of AuNPs powder was added, stirred at room temperature for 2 h, washed repeatedly and separated by magnetic hysteresis, and vacuum dried at 50 °C for 7 h to obtain Fe 3 o 4 -AuNPs;

[0085] 2) 3D-rGO / Fe 3 o 4 - Preparation of AuNPs nanomaterials:

[0086] First, graphene oxide (GO) (40mg / 20mL) was ultr...

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Abstract

The present invention provides a 3D-rGO / Fe 3 o 4 Preparation method and application of ‑AuNPs / HP‑β‑CD composites. The preparation of the composite material is by Fe 3 o 4 and AuNPs and hydroxypropyl-β-cyclodextrin (HP-β-CD) modified on the three-dimensional graphene surface prepared (3D-rGO / Fe 3 o 4 ‑AuNPs / HP‑β‑CD). The material not only combines the large specific surface area of ​​3D‑rGO, fast electron transfer rate, Fe 3 o 4 ‑AuNPs have the advantages of high electrocatalytic activity and the excellent molecular recognition ability of HP‑β‑CD. A series of characterizations have confirmed that Fe 3 o 4 , AuNPs and HP‑β‑CD were successfully loaded in the three-dimensional graphene structure and modified onto the surface of glassy carbon electrodes to prepare a new sensing platform for the simultaneous detection of guanine and adenine. The results showed that guanine and adenine obtained lower detection limits in a wider linear range, which greatly improved the sensitivity of the modified electrode for the detection of the two kinds of purines. The sensor prepared by the invention is also successfully used for the simultaneous detection of guanine and adenine in the sperm DNA of actual samples.

Description

technical field [0001] The invention belongs to nanocomposite materials and electrochemical detection, in particular to a 3D-rGO / Fe 3 o 4 - Preparation of AuNPs / HP-β-CD nanocomposite and its modification to the electrode surface for simultaneous detection of guanine and adenine. Background technique [0002] Guanine (G) and adenine (A) are two important bases in deoxynucleic acid (DNA) and nucleic acid (RNA). As a biomolecule, purine not only has multiple important functions in cell proliferation, but also exists as a danger signal in the process of cell lysis and apoptosis. The concentration of bases in DNA will affect the activity of enzymes. By changing the normal purine metabolism pathway, it will affect the immune system and cause various diseases such as obesity and tumor recurrence. Therefore, accurate and efficient detection of the content of guanine and adenine in biological samples is of great significance in the fields of biological science and analytical chemi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 梁文婷张成玲戎艳琴马学文芦冬涛董川双少敏
Owner SHANXI UNIV
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