Respiratory tract infection multiple detection reagent kit and detection method

A multiplex detection and kit technology, applied in the biological field, can solve the problems of false negatives, low specificity and sensitivity, and low sensitivity

Active Publication Date: 2019-09-24
DAAN GENE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is complex, cumbersome to operate, long cycle, low sensitivity, relatively high requirements for samples, and prone to false negative results
[0006] Serological testing is based on the pri

Method used

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  • Respiratory tract infection multiple detection reagent kit and detection method
  • Respiratory tract infection multiple detection reagent kit and detection method
  • Respiratory tract infection multiple detection reagent kit and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0126] 1. Materials and methods

[0127] 1.1 Simulation samples

[0128] Virus liquid: Influenza A virus, Influenza B virus, Respiratory syncytial virus; Plasmids: Adenovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Legionella pneumophila, Haemophilus influenzae, plasmids by Shanghai Sangon Bioengineering Co., Ltd., and the virus liquid was prepared by Daan Gene Co., Ltd. of Sun Yat-sen University.

[0129] 1.2 Clinical samples

[0130] 50 positive samples for respiratory pathogens and 20 negative samples.

[0131] 1.3 Primers and probes

[0132] Through the sequence alignment analysis of the respiratory pathogen-related nucleic acid sequences reported in the Genebank database and domestic and foreign published literature, the segment without secondary structure and highly conserved was selected as the target fragment for amplification, and multiple pairs of primers and probes were manually designed. Both primers and probes were synthesized b...

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Abstract

The invention provides a respiratory tract infection multiple detection reagent kit and detection method. Particularly, a multiplex respiratory tract pathogen PCR amplification system is designed and subjected to experimental verification, so that a multiplex fluorescent PCR amplification system for detecting various respiratory tract pathogens is obtained, and a single system can detect 3 kinds of respiratory tract pathogens at the same time. The reagent kit has high sensitivity and specificity. Through the reagent kit, quick detection and analysis of multiplex respiratory tract pathogens in samples of nasopharyngeal swabs, bronchoalveolar lavage fluid, phlegm and the like can be realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular, the invention relates to a multiple detection kit and detection method for respiratory tract infection. Background technique [0002] Acute respiratory tract infection (ARTI) is one of the most common infectious diseases in humans worldwide, and its morbidity ranks first among acute infectious diseases. On average, lower respiratory tract infections cause about 4,000,000 deaths each year. Globally, respiratory infections are the leading cause of death in children under the age of 5. ARTIs are generally caused by bacteria and viruses, of which about 90% are caused by respiratory viruses, but death and severe cases are mainly caused by bacterial infections. Faced with the current complex situation of prevention and control of respiratory infectious diseases, strengthening the monitoring of epidemic information of infectious diseases and strains can provide a basis for formulating pr...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/689C12Q1/04C12N15/11C12R1/21C12R1/46C12R1/01
CPCC12Q1/701C12Q1/689C12Q2600/16
Inventor 蒋析文黄桃生李欣钰郑若楠
Owner DAAN GENE CO LTD
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