A Salt-tolerant Denitrifying Bacteria and Its Application

A denitrifying bacteria and salt-tolerant technology, applied in the field of environmental microorganisms, can solve problems such as the inability to remove nitrite nitrogen

Active Publication Date: 2021-04-23
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention aims to solve the technical problem that the nitrogen removal ability of the existing common denitrifying bacteria is inhibited by high salinity and cannot remove nitrite nitrogen in the saline aquaculture wastewater, and provides a salt-tolerant denitrifying bacteria and its application

Method used

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  • A Salt-tolerant Denitrifying Bacteria and Its Application
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  • A Salt-tolerant Denitrifying Bacteria and Its Application

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specific Embodiment approach 1

[0025] Specific embodiment 1: In this embodiment, a salt-tolerant denitrifying bacterium, which is Alishewanella sp.F2, is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is Chaoyang, Beijing No. 3, No. 1 Yard, Beichen West Road, District. The preservation date was March 25, 2019, in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the number is CGMCC No: 17433.

[0026] The Alishewanella sp.F2 colony in this embodiment is white and round, with a diameter of 1-2 mm, neat edges, smooth surface, sticky and shiny. Observed under a microscope, the bacteria are rod-shaped, with a length of 1-2 μm and a width of 0.3-0.5 μm.

[0027] The physiological and biochemical characteristics of Alishewanella sp.F2 in this embodiment are as shown in Table 1:

[0028] Table 1 Physiological and biochemical characteristics of Alishewanella sp.F2

...

specific Embodiment approach 2

[0031] Specific embodiment two: the screening method of Alishewanella sp.F2 in this embodiment is realized according to the following steps:

[0032] Step 1: Take 2mL of muddy water samples collected from the seawall of Zhuanghe City, Dalian City, Liaoning Province (39°38′31″N latitude 122°58′19″East longitude) and put it into a 250mL conical flask, add 200mL strains for denitrification culture The bottle mouth is sealed with a sealing film to create an anoxic environment; the enriched culture solution is obtained after standing at a constant temperature for 5 to 7 days at a temperature of 30°C;

[0033]Step 2: Gradiently dilute the enriched culture solution in Step 1 using the doubling dilution method, pipette 0.5mL of each dilution suspension into the strain screening plate culture medium (which has been placed overnight, no bacteria growth), and coat with a glass triangle. After spreading the enriched culture solution evenly with a cloth stick, pour the second layer of ster...

specific Embodiment approach 3

[0052] Specific embodiment three: In this embodiment, the determination of the salt tolerance of Alishewanella sp.F2 is as follows:

[0053] Prepare screening and separation media with different salinity gradients, that is, the NaCl concentrations are 0g / L, 30g / L, 50g / L, 70g / L, and 100g / L; inoculate strain F2 onto the medium plates of various salinities Put it into a constant temperature incubator at 30°C and cultivate it for 5 days. Whether the strain grows is used as the basis for whether the strain can withstand the corresponding salinity; the culture medium not inoculated with denitrifying bacteria is used as the blank control, and each group has 3 parallels.

[0054] After 1 day of culture, no colonies appeared in the medium of each salinity; after 2 days of culture, there were obvious colonies on the medium containing 0g / L and 30g / L NaCl, containing 50g / L, 70g / L and 100g / L There was a small amount of colony growth on the medium containing L NaCl; after 5 days of cultivat...

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Abstract

A salt-tolerant denitrifying bacterium and its application, the invention relates to the technical field of environmental microorganisms. The invention aims to solve the technical problem that the nitrogen removal ability of the existing common denitrifying bacteria is inhibited by high salinity and cannot remove the nitrite nitrogen in the saline culture wastewater. The strain is Alishewanella sp.F2, which was deposited in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures. The preservation date was March 25, 2019. The number is CGMCC No: 17433. The strain can denitrify and denitrify under saline conditions. The bacterial strain of the invention is used for removing nitrite nitrogen pollutants in water bodies.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms. Background technique [0002] With the rapid development of marine aquaculture, most of them have entered the form of semi-intensive or intensive aquaculture. Many factors such as high stocking density and excessive feeding residual bait have caused the increase of nitrite nitrogen in the aquaculture water year by year. Nitrite nitrogen is not only one of the eutrophication factors in water bodies, but also an intermediate product of nitrification and denitrification, so it widely exists in natural water bodies and is likely to cause great harm to aquatic animals and human health. Nitrite in water is highly toxic. Humans drinking high-concentration nitrite water for a long time will lead to mental retardation and even death; aquatic animals are more sensitive to nitrite toxicity due to the accumulation of nitrite, resulting in insufficient oxygen content in aquatic animal tis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16C02F103/20
CPCC02F3/34C02F2101/166C02F2103/20C12N1/205C12R2001/01
Inventor 祝惠王鑫壹阎百兴于翔霏陈欣
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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