Nosocomial Acinetobacter fk2 and its application in degrading organic pollutants
A technology of organic pollutants, FK2, applied in the direction of water pollutants, microorganism-based methods, bacteria, etc., to achieve the effect of high-efficiency degradation ability
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Embodiment 1
[0034]Example 1: Isolation, purification and identification of Acinelobacter nosocomialis FK2.
[0035] 1. Isolation and purification of Acinelobacter nosocomialis FK2
[0036] Acinelobacter nosocomialis FK2 is a Gram-negative bacterium domesticated and isolated from the activated sludge in the aeration tank of an urban sewage treatment plant. The mixed liquor suspended solids concentration MLSS in activated sludge is 2300mg / L, and the mixed liquor volatile suspended solids concentration MLVSS is 1820mg / L. The specific steps of screening are as follows:
[0037] Add 50 mL of inorganic salt culture solution to a 300 mL shake flask, and add 10 mL of activated sludge and isoamyl acetate with a final concentration of 500 mg / L for enrichment culture. When the concentration of isoamyl acetate is 50% of the initial value, 5 mL of rich Collect the solution in 50mL of fresh inorganic salt culture solution, add the same amount of isoamyl acetate, repeat the above enrichment process 5 ...
Embodiment 2
[0048] Example 2 Acinetobacter FK2 resting cell acquisition in hospital
[0049] (1) Incline cultivation:
[0050] Inoculate the hospital Acinetobacter FK2 into LB liquid medium, culture at 30°C, 160rpm for 2 days, then line the activated bacteria on a solid LB plate in a 30°C incubator, take a single colony and continue to line the plate to detect bacteria The purity of the LB test tube was stored on a slant plane (4°C).
[0051] (2) Expansion of training
[0052] Inoculate the slant bacteria from step (1) into LB liquid medium, cultivate at 30°C and 160rpm for 12 hours, obtain the expanded culture solution, centrifuge, collect the wet bacteria, wash with the inorganic salt culture solution, and obtain the Acinetobacter hospital FK2 static information cells.
Embodiment 3
[0053] Example 3: Detection of the degradation performance of hospital Acinetobacter FK2 to different concentrations of isoamyl acetate
[0054] Divide the inorganic salt culture solution into 330mL shake flasks each with a volume of 50mL, and sterilize at 110°C for 40min. After the sterilization, it was placed at room temperature for 2 days to confirm that there was no growth of miscellaneous bacteria. Add the Acinetobacter acetobacter FK2 quiescent cells in hospital that the final concentration reaches 30mg / L (recorded in dry cell weight) embodiment 2 method, then add isoamyl acetate as the sole carbon source to make the final concentration respectively 100,200,400 , 600, 800, 1000, 1200, 1400mg / L, after the shake flask was sealed with a polytetrafluoroethylene stopper, cultured on a shaker at 30°C at 160rpm, and a blank control without bacteria was made. Regularly measure the residual isoamyl acetate concentration in the shake flask, draw the removal rate curve of bacteria...
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