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Dog vaccine, preparation method thereof and application of dog vaccine

A vaccine, canine distemper technology, applied in the field of canine vaccines and its preparation, can solve the problems of reducing the number of vaccine immunizations for canine animals, and achieve the effect of being suitable for large-scale industrial production, convenient operation, and reducing the number of immunizations

Active Publication Date: 2019-10-08
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The first object of the present invention is to provide a vaccine for dogs, which can simultaneously prevent and treat canine distemper, parvovirus enteritis and rabies through one immunization, and can effectively reduce the cost of canine vaccines. Increase the number of immunizations, thereby alleviating the problem that the existing single vaccine requires repeated immunizations in the process of canine immunization prophylaxis

Method used

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  • Dog vaccine, preparation method thereof and application of dog vaccine
  • Dog vaccine, preparation method thereof and application of dog vaccine
  • Dog vaccine, preparation method thereof and application of dog vaccine

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preparation example Construction

[0072] According to one aspect of the present invention, a kind of preparation method of above-mentioned canine vaccine, described preparation method comprises the following steps:

[0073] Firstly, the immunizing amount of canine distemper virus antigen, the immunizing amount of canine parvovirus antigen and the immunizing amount of rabies virus antigen are mixed evenly, and then a veterinary acceptable adjuvant is added to prepare the canine vaccine.

[0074] The preparation method of the canine vaccine provided by the invention, the preparation method first mixes the immunizing amount of canine distemper virus antigen, the immunizing amount of canine parvovirus antigen and the immunizing amount of rabies virus antigen, and then adds veterinary acceptable Adjuvant, prepared dog vaccine. The method has the advantages of simple preparation process, convenient operation and suitable for large-scale industrial production.

[0075] In a preferred embodiment of the present invent...

Embodiment 1

[0086] Example 1 Culture and propagation of canine parvovirus virulent strain JL(18)1-Beagle

[0087] Wait for T75cm 2 When the cat kidney cell F81 grows into monolayer cells in the cell culture flask, inoculate the preserved canine parvovirus virulent strain 1ml JL(18)1-Beagle(10 6.0 TCID50 / mL), and placed at 35°C 5% CO 2 Carry out the virus amplification culture in the medium, observe the cell virus situation every day, when the cytopathic change reaches about 80%, can repeatedly freeze and thaw 3 times, harvest the virus liquid, and carry out the virus content (≥10 6.0 TCID50 / mL), sterility test, mycoplasma test and exogenous virus test, and the virus liquid that passed the test was stored at -80°C;

Embodiment 2

[0088] Example 2 Culture Proliferation of Canine Distemper Virus Strong Strain LN(10)1

[0089] Wait for T75cm 2 When the African green monkey kidney cells VDS expressing the canine SLAM receptor in the cell culture flask grow into a single layer of cells, inoculate 1ml LN(10)1(10 6.0 TCID50 / mL), and placed at 35°C 5% CO 2 Carry out the virus amplification culture in the medium, observe the cell virus situation every day, when the cytopathic change reaches about 80%, can repeatedly freeze and thaw 3 times, harvest the virus liquid, and carry out the virus content (≥10 6.0 TCID50 / mL), sterility test, mycoplasma test and exogenous virus test, and the virus liquid that passed the test was stored at -80°C;

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Abstract

The invention provides a dog vaccine, a preparation method thereof and an application of the dog vaccine, and relates to the technical field of veterinary drug biological products. The dog vaccine comprises an immune dosage canine distemper virus antigen, an immune dosage canine parvovirus antigen and an immune dosage rabies virus antigen, wherein the canine distemper virus antigen comprises a canine distemper virulent strain LN (10)1 in an inactivated form, the canine parvovirus antigen comprises a parvovirus virulent strain JL (18)1-Beagle in an inactivated form, and the rabies virus antigencomprises a rabies virus attenuated strain HEP-Flury. The vaccine can achieve the effect of simultaneously controlling canine distemper, canine parvovirus enteritis and rabies by one-time immunization, can effectively reduce the immunization frequency of canine vaccines and further can relieve the problems that working intensity is increased and stress response of canine animals is easily causedas an existing single vaccine needs repeated immunization in the immunological prevention process of the canine animals.

Description

technical field [0001] The invention relates to the technical field of veterinary medicine biological products, in particular to a dog vaccine and its preparation method and application. Background technique [0002] Canine distemper is an acute, febrile, highly contagious infectious disease caused by Canine Distemper Virus (CDV) of the Paramyxoviridae genus Morbillivirus. Mainly, there are biphasic elevated body temperature, accompanied by catarrhal pneumonia, gastroenteritis, and occasionally neurological symptoms. It is prone to secondary mixed infection and secondary infection of other bacteria and viruses, and the mortality rate can be as high as 80%. Virus-carrying animals are the most dangerous source of infection. Viruses are excreted through eye and nasal secretions, saliva, urine, and feces, and are mainly transmitted through the digestive tract and respiratory tract. In recent years, with military dogs, police dogs, experimental dogs and pet dogs With the substant...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/205A61K39/175A61K39/23A61P31/14A61P31/20
CPCA61K39/12A61P31/14A61P31/20C12N2760/18434C12N2760/20134C12N2750/14334A61K2039/552A61K2039/70A61K2039/5252
Inventor 陈杰赵建军
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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