Method for increasing body fat content

Abstract

The invention discloses a method for increasing the body fat content. The method comprises the following steps that S1, body subcutaneous fat tissue is taken under aseptic conditions, the body subcutaneous fat tissue is transferred into a plate pre-filled with a D-Hanks solution, rinsing is performed for 5-8 times, and ophthalmic scissors are used for removing visible blood vessels and connectivetissue in the fat tissue; S2, a tissue block is cut into three pieces of small blocks of 8-10mm, and homogenate is performed with a tissue homogenizer at 180-200r / min to achieve a chyle shape; then the chyle-shaped tissue is added into a 0.1% collagenase I medium for digestion for 30 min, and vibration is performed once every 5 min during digestion; and S3, undigested tissue blocks and fat cells of large sizes are filtered and removed through a 200-mesh stainless steel cell sieve, and cell suspension is transferred into a centrifuge tube and centrifuged at 2000 r / min for 5 min; and supernatantafter centrifugation is removed. According to the method for increasing the body fat content, in an adipose cell population treated with a sterile membrane stabilizing agent, PRP and recombinant human VEGF-C, the survival rate of grafts of the adipose cell population before, during or after delivery or administration to a subject can be increased, and the body fat content is greatly increased.

Description

technical field [0001] The invention relates to the technical field of adipocyte culture, in particular to a method for increasing body fat content. Background technique [0002] The angiogenesis process relies on the activity of various growth factors, for example, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF). [0003] Vascular endothelial growth factor (VEGF) is a secreted glycoprotein that plays a role in maintaining the original blood vessel density and maintaining the permeability of blood vessels necessary for transporting nutrients. The biological effects of VEGF include: promoting the proliferation of endothelial cells, VEGF can induce endothelial cells to express proteolytic enzymes, interstitial collagenase and tissue factor, degrade extracellular matrix, make vascular endothelial cells grow inward, and induce angiogenesis. To improve the permeability of blood vessels, Roberts et al. fou...

AI Technical Summary

Problems solved by technology

However, the relatively high resorption rate of fat grafts reduces the efficacy of this technique due to increased fat cell death after transplantation

Although angiogenic factors (Kuramochi et al., 2008), and VEGF gene therapy (Lei et al., 2008) have been used alone to stimulate angiogenesis in fat grafts to improve adipocyte survival and viability, clinical outcomes have been poor. unsatisfactory

Therefore, reducing the reabsorption rate of grafted fat is a clinical challenge