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Construction of a Photoelectrochemical Sensor for Detection of Prostate Specific Antigen

A photoelectrochemical, prostate-specific technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve the effect of photoelectric signal enhancement and photoelectric signal reduction

Active Publication Date: 2021-06-29
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these analytical systems often suffer from inherent drawbacks of limited sensitivity

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  • Construction of a Photoelectrochemical Sensor for Detection of Prostate Specific Antigen
  • Construction of a Photoelectrochemical Sensor for Detection of Prostate Specific Antigen

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Embodiment 1

[0029] A method for fast detection of PSA is characterized in that it comprises the following steps:

[0030] (1) Ultrasonic cleaning the titanium sheet with acetone, ethanol and ultrapure water for 10 min each, the titanium sheet was used as the anode, and the graphite sheet was used as the cathode, containing 0.3 wt % NH 4 Ethylene glycol with F and 2.0 vol% water was used as the electrolyte solution, oxidized for 2 h under the condition of an external constant voltage of 40V, the titanium sheet was taken out and washed with ultrapure water, dried to obtain amorphous titanium dioxide, and calcined at 450 °C for 2 h After cooling to room temperature for h to obtain anatase titanium dioxide nanotubes, the working area of ​​the titanium dioxide nanotube electrode is 1 cm×1 cm.

[0031] (2) Add 120 mL of 5 mmol / L CdCl 2 solution and 90 μL mercaptopropionic acid solution were added to a three-necked flask, and the pH of the solution was adjusted to 11.8 with 1.0 mol / L NaOH, and ...

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Abstract

Construction of a photoelectrochemical sensor for prostate-specific antigen detection. In the present invention, the anatase titanium dioxide nanotube electrode is prepared by anodic oxidation method, TiO 2 Nanotube electrodes form CdTe / TiO via electrostatic adsorption of CdTe quantum dots 2 structure, immobilized on its surface a specific peptide that can be cleaved by prostate-specific antigen, using 1‑(3‑dimethylaminopropyl)‑3‑ethylcarbodiimide hydrochloride and N‑hydroxysuccinimide DNA was ligated, double-helix DNA was used as a carrier to immobilize the doxorubicin-CuS complex, and CuS nanocrystals were used as a signal quencher to establish a sensitive peptide-based photoelectrochemical biosensor for the quantitative detection of prostate-specific antigen. The photoelectrochemical biosensor has good specificity, stability and reproducibility, and has potential applications in bioanalysis, disease diagnosis and clinical biomedicine.

Description

technical field [0001] The invention relates to a detection method of prostate-specific antigen (PSA) in blood, specifically, a photoelectrochemical biosensor for measuring PSA content in blood is constructed based on photoelectrically active inorganic semiconductor materials and a specific peptide. Background technique [0002] Prostate-specific antigen is a serine protease secreted by the prostate. The content of PSA in serum has a great effect on the diagnosis of prostate cancer. Generally speaking, it is normal when the PSA level in the serum is less than 4.0 ng / mL. When prostate cancer occurs, the PSA level exceeds 10 ng / mL. According to the amount of PSA, it is helpful to judge whether the disease is diseased. So far, many detection methods, such as chemiluminescence, fluorescence analysis and electrochemical analysis, have been used to measure PSA in blood. However, these analytical systems often suffer from inherent drawbacks of limited sensitivity. Therefore, it i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3278
Inventor 葛慎光赵金歌王绍鹏王芳芳李娜付翠萍于京华颜梅
Owner UNIV OF JINAN