Composition for preventing hair loss and promoting hair growth, comprising fermented marine animal products
A technology for aquatic animals and fermented products, applied in application, food science, hair care, etc., to achieve the effect of no toxicity and side effects, safe and long-term use
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0068] Embodiment 1: the preparation of fermented fish oil
[0069] The fermented fish oil used as the experimental material was supplied by Qinglong Aquatic Products Co., Ltd. Specifically, the by-products produced when processing five types of aquatic products including mackerel, horsehead fish, puffer fish, and flounder at Qinglong Fisheries Co., Ltd.'s fishery processing plant, which is frozen and stored in units of 10 kg for each type, were used. The above-mentioned raw materials were put into a stainless steel fermentation vessel with a weight of 50 Kg, and 10 Kg of commercially available sucrose and fermenting microorganisms were added as a culture substrate. As yeast and lactic acid bacteria as fermenting microorganisms, inoculate 2L of pre-cultured Lactobacillus plantarum (Lactobacillus plantarum) (1.0×10 8 cells / cfu) and Saccharomyces cerevisiae (3.0×10 8 cells / cfu), cultured at 30°C for 15 days, and then fermented for 50-60 days. After the cultivation and ferment...
Embodiment 2
[0072] Example 2: Confirmation of the proliferation-promoting effect of fermented fish oil on dermal papilla cells (DPCs)
[0073] Using DMEM (Hyclone Inc, USA) medium containing 100units / ml penicillin-100μg / ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal bovine serum (FBS; Gibco Inc, NY, USA), in 37°C temperature in 5% CO 2 Immortalized dermal papilla cells (Rat vibrissa immortalized dermal papilla cells: Filsell W, et al., 1994) isolated from whiskers of white mice were cultured in a thermostat, and subcultured every three days. Proliferation of dermal papilla cells was measured by MTT assay. Dermal papilla cells (dermal papilla cell) 5.0×10 4 The cells / ml were cultured in DMEM medium containing 1% FBS, put into a 24-well plate (well plate), and after culturing for 24 hours, processed the fermented fish oil at concentrations of 12.5 μg / ml, 25 μg / ml and 50 μg / ml. As a positive control, minoxidil (Sigma, MO, USA) was treated at a concentration of 10 μM. ...
Embodiment 3
[0075] Example 3: Measurement of hair growth efficacy of fermented fish oil
[0076] Six-week-old female C57BL / 6 mice were purchased from Orient Bio Co., Ltd., and used for experiments after acclimatization in the animal breeding room environment for one week. The temperature in the animal breeding room was 23±3°C, and the day and night lasted for 12 hours respectively. Experimental animals were raised in isolated mouse cages and fed with experimental feed freely. The experimental group consists of 5 groups, which are the control group and the positive control group MINOXYL TM (5% Minoxidil) (modern drug) and fermented fish oil (10 μg / ml, 50 μg / ml, 100 μg / ml; solvent is 50% ethanol) coating group. In order to observe the hair-raising effect, a 7-week-old telogen shaved back skin was used. The hair on the back of the mouse was completely shaved using a clipper for animals, and after 2 days, the mouse that was visually recognized as having no damage on the skin was selected f...
PUM
Property | Measurement | Unit |
---|---|---|
Concentration | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com