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Preparation method and application of antibiotic molecular imprinting adsorption membrane

A technology of molecular imprinting and antibiotics, which is applied in the field of pollutant control and detection, can solve the problems of non-selectivity and decline in sampling performance, and achieve the effect of low cost, simple operation and good hydrophilicity

Inactive Publication Date: 2019-10-15
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The binding phase is a key part of DGT, and the DGT binding phases currently used for organic matter include C18, HLB, XAD-2, XAD-18, etc. (Chen.C, et al.J.Environ.Monit., 2012,14,1523- 1530; Tankéré-MullerSophie, et al.J.Anal.Chim.Acta,2012,716:138-144;Bennett W.W,et al.,Anal.Chem.2011,83:8293~8299;Qin Sun,et al. J.Anal.Chem.2014,86,3060-3067.), these binding phases are not selective, and when sampling in complex environmental conditions, the interference of other substances will lead to a decline in sampling performance

Method used

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  • Preparation method and application of antibiotic molecular imprinting adsorption membrane
  • Preparation method and application of antibiotic molecular imprinting adsorption membrane
  • Preparation method and application of antibiotic molecular imprinting adsorption membrane

Examples

Experimental program
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Effect test

Embodiment 1

[0033] With baloxacin as a template molecule, methacrylic acid as a functional monomer, and divinylbenzene as a crosslinking agent, it was dissolved in 100 mL of acetonitrile according to a molar ratio of 1:4:12, and 120 mg of azobisisobutyronitrile was added to the solution , passing nitrogen gas to remove oxygen, reacting at 60° C. at a speed of 100 rpm for 8 hours, centrifuging to collect precipitates, eluting template molecules, and drying to obtain fluoroquinolone antibiotic molecularly imprinted materials.

[0034] Prepare an agarose solution with a mass concentration of 1%, mix the fluoroquinolone antibiotic molecularly imprinted material with the agarose solution at a ratio of 0.4%, and quickly inject it into a glass plate with a plastic septum (thickness 0.5mm) on the edge, after the air bubbles are discharged , placed at room temperature for 1 hour, after the solution was gelled, the glass plate and septum were removed, soaked in ultrapure water for 24 hours, and cut ...

Embodiment 2

[0036] With sulfadoxine as a template molecule, methacrylic acid as a functional monomer, and divinylbenzene as a crosslinking agent, it is dissolved in 100mL of acetonitrile according to a molar ratio of 1:4:12, and 110mg of azobisisobutyronitrile is added to the solution. Nitrogen was introduced to remove oxygen, reacted at 60° C. for 16 hours at 150 rpm, centrifuged to collect precipitates, eluted template molecules, and dried to obtain sulfonamide antibiotic molecularly imprinted materials.

[0037] Prepare an agarose solution with a mass concentration of 1.5%, mix the sulfonamide antibiotic molecularly imprinted material with the agarose solution at a ratio of 1%, and quickly inject it into a glass plate with a plastic septum (thickness 0.5mm). After the solution is gelled, remove the glass plate and septum, soak in ultrapure water for 30 hours, take it out and cut it into a sulfonamide antibiotic molecularly imprinted adsorption membrane with a diameter of 2 cm.

Embodiment 3

[0039] Prepare an agarose solution with a mass concentration of 3%, mix the fluoroquinolone antibiotic molecularly imprinted material prepared in Example 1 with the sulfonamide antibiotic molecularly imprinted material prepared in Example 2 and mix it with the agarose solution at a ratio of 3%, and quickly Pour into a glass plate with a plastic septum (thickness 0.8mm) on the edge, let it stand at room temperature for 5 hours after the air bubbles are discharged, and after the solution gels, remove the glass plate and septum, and soak in ultrapure water for 32 hours , taken out and cut into a 2.5cm diameter antibiotic molecularly imprinted adsorption membrane (such as figure 1 shown).

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Abstract

The invention relates to a preparation method and application of an antibiotic molecular imprinting adsorption membrane, and belongs to the fields of pollutant control and detection. An antibiotic molecularly imprinted membrane is prepared by doping an antibiotic molecularly imprinted material into agarose gel, is capable of selectively adsorbing antibiotic contaminants in a water body, and servesas a binding phase of diffusion gradient membrane technology for antibiotic determination in the water body. The preparation method comprises the following steps: preparing the antibiotic molecularlyimprinted material firstly, then mixing the molecularly imprinted material with an agarose solution in a certain ratio, injecting the mixture into a glass plate with a U-shaped plastic insulation pad, placing at room temperature and immersing into gel to form the antibiotic molecular imprinting adsorption membrane. The method provided by the invention is simple to operate and low in cost; the obtained molecularly imprinted membrane is good in hydrophilicity, high in adsorption capacity on antibiotics in the water body and good in selectivity, can be used for high-efficiency adsorption removalof antibiotics in the water body, and serves as a binding phase of diffusion gradient membrane technology for antibiotic determination in the water body.

Description

technical field [0001] The invention relates to a preparation method and application of an antibiotic molecularly imprinted adsorption film, belonging to the fields of pollutant control and detection. Background technique [0002] As a new type of pollutant, antibiotics in the environment have been widely detected in water bodies and sediments. Antibiotics in the environment will not only increase the resistance of bacteria, but also be ingested by the human body through drinking water and the food chain, posing a threat to human health. Generally, the concentration of antibiotics in water is low (μg / L or ng / L level), and it is difficult to remove them by sewage treatment plants using conventional treatment processes. Adsorption is a technology to remove low-concentration pollutants, but it is currently facing great challenges to selectively remove low-concentration target pollutants under the condition of a large number of coexisting organic / inorganic substances. [0003]...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30C02F1/28
CPCB01J20/261B01J20/28033C02F1/285
Inventor 谭峰崔颖任苏瑜
Owner DALIAN UNIV OF TECH
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