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Method for breeding high-yield high-quality stress-resistant good new common andrographis herb varieties

A technology for andrographis paniculata and new varieties is applied in the field of breeding high-yield, high-quality, stress-resistant and fine new varieties of Andrographis paniculata.

Active Publication Date: 2019-10-22
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to overcome the degradation of Andrographis paniculata varieties in the above-mentioned prior art, the problems of yield and quality decline, and provide a method for breeding new varieties of Andrographis paniculata with high yield, high quality, high quality and stress resistance

Method used

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  • Method for breeding high-yield high-quality stress-resistant good new common andrographis herb varieties
  • Method for breeding high-yield high-quality stress-resistant good new common andrographis herb varieties
  • Method for breeding high-yield high-quality stress-resistant good new common andrographis herb varieties

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Experimental program
Comparison scheme
Effect test

Embodiment 160

[0029] Example 1 60 Effect of Co-γ-Ray on Yield per Mu of Andrographis paniculata

[0030] 1. Method

[0031] Select the plump and uniform seeds of Andrographis paniculata, wash them with deionized water, remove the suspended grains and impurities, disinfect them with 1% sodium hypochlorite for 20 minutes, rinse them with distilled water for 3 times, use filter paper to dry the attached water, and use different doses of 60 After irradiation with Co-γ rays (0, 10, 20, 50, 100, 200, 300Gy), 100 seeds of Andrographis paniculata in each irradiation dose group were taken and soaked in distilled water for 24 hours. The Andrographis paniculata seeds of each dose group were sown in 50 hole seedling pots, one hole for each seed, and two seedling pots for each group. Every day at 12 o'clock noon and 5 o'clock in the afternoon, the water in the seedling pots was supplemented to keep the culture soil moist, and the growth of the Andrographis paniculata seedlings was observed. When the t...

Embodiment 260

[0037] Example 260 Effects of Co-γ-rays on the Content of Active Components in Andrographis paniculata

[0038] 1. Method

[0039] Select Andrographis paniculata plants in good growth condition, and measure the content of active ingredients by pharmacopoeia method: using octadecylsilane bonded silica gel as filler; using methanol-water (52:48) as mobile phase; andrographolide detection wavelength is 225nm, and the detection wavelength of dehydroandrographolide is 254nm. The number of theoretical plates should be no less than 2000 based on the peaks of andrographolide and dehydroandrographolide. Take an appropriate amount of andrographolide reference substance and dehydroandrographolide reference substance, weigh them accurately, add methanol to make a mixed solution containing 0.1mg per 1mL, and obtain. Get about 0.5 g of the Andrographis paniculata plant sample powder (passed through a No. 4 sieve) through radiation mutagenesis treatment in Example 1, accurately weigh it, p...

Embodiment 3

[0046] Embodiment 3 andrographis paniculata salt tolerance experiment

[0047] 1. Salt stress experiment

[0048] (1) Method

[0049] The salt content was set as (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0%) NaCl solution (g / mL). Andrographis paniculata seeds were soaked in NaCl solutions of different concentrations for 24 hours, and the moisture on the surface was blotted with filter paper after taking them out. Put the soaked seeds evenly into petri dishes covered with 2 layers of filter paper, add 5mL of NaCl solution of corresponding concentration to each petri dish (the concentration of salt solution is consistent with the concentration of soaking seeds); put the petri dish in the light incubator For cultivation, the temperature is 25°C, the light intensity is 10000-12000lx, and the light time is 12h light / 12h dark. There were 50 seeds in each treatment group, and 3 replicates were set up, and distilled water was supplemented once a day to ensure that each sal...

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Abstract

The invention discloses a method for breeding high-yield high-quality stress-resistant good new common andrographis herb varieties. The method includes the steps: taking common andrographis herb seedsas raw materials, taking 60 Co-gamma rays as radiation sources, radiating the common andrographis herb seeds, dividing the radiated seeds into three groups, soaking the seeds by distilled water, germinating the seeds in the salt stress environments containing NaCl solution and the drought stress environments containing PEG 6000 solution, and calculating germination rate, germination potential, germination indexes, vigor indexes, root length and bud length or fresh weight; selecting new salt-tolerant and drought-resistant high-yield high-quality stress-resistant varieties according to germination rate, germination potential, germination indexes, vigor indexes, root length and bud length or fresh weight of each sample. According to the method, the common andrographis herb seeds are radiatedby the aid of suitable dosages of 60 Co-gamma rays, good tolerance improvement methods of common andrographis herbs under salt stress and drought stress are expected to be achieved in practice, and application potential is high. According to the method, injury of adverse situations to plants is relieved, and the method has high application values.

Description

technical field [0001] The invention belongs to the technical field of biological planting. More specifically, it relates to a method for breeding a new variety of Andrographis paniculata with high yield, high quality, high quality and stress resistance. Background technique [0002] Andrographis paniculata (Burm.f.) Nees is derived from Andrographis paniculata (Burm.f.) Nees, a plant of the Acanthaceae family. The aboveground part is used as medicine. Due to its long-term artificial cultivation, the phenomenon of species degradation, yield decline, and active ingredient content reduction are serious. , High-quality new germplasm breeding research is imminent. Radiation mutagenesis is mainly to treat materials with a certain dose of radiation, so as to cause mutations in the materials, induce mutations, and finally select and breed new varieties that are useful for scientific research or production from the mutant materials after screening, measurement, and selection. proc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 杜勤苏雨苗王振华刘潇晗石志棉
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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