Oriental latrine fly larvina composition and application thereof in relaxing bowels and treating habitual and senile constipation
A technology for laxative and grain worms is applied in the directions of drug combinations, medical preparations containing active ingredients, medical raw materials derived from arthropods, etc., to achieve the effect of improving the promoting effect and improving the laxative effect
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Embodiment 1 5
[0031] The preparation of embodiment 1 grain insect powder
[0032] The preparation of each insect powder was carried out using the same batch of multiplied grain worms in the same container.
[0034] Weigh the grain worms, wash them, and mash them; add water three times the weight of the worm bodies, add chitinase and alkaline protease according to the amount of 50 U / g worm bodies and 20 U / g worm bodies, and treat for 2 hours at room temperature; filter Remove water, stir-fry over low heat until golden brown; crush and pass through a 50-mesh sieve.
[0035] Bug Meal 2:
[0036] Weigh, wash and mash the grain worm; add water three times the weight of the worm body, add chitinase according to the amount of 50U / g worm body, and treat it at room temperature for 2 hours; filter out the water, stir-fry until golden yellow; crush Pass through a 50-mesh sieve.
[0037] Bug Meal 3:
[0038] Weigh the grain worms, wash them, and dry them in the sun; stir-fr...
Embodiment 2 5
[0039] Example 2 Effect of grain insect powder on intestinal flora of mice
[0040] C57BL / 6 mice were purchased from Shanghai Kaixue Biotechnology Co., Ltd.
[0041] A total of 80 healthy mice with good diet and activity status were selected and randomly divided into 7 groups. 7 groups were given 0.5mg / worm powder 1, 0.25mg / worm powder 1, 0.5mg / worm powder 2, 0.25mg / worm powder 2, 0.5mg / worm powder 3, 0.25mg / worm powder 3. 0.5mg insect powder 1+0.2mg xylo-oligosaccharide / piece, 0.25mg insect powder 1+0.2mg xylo-oligosaccharide / piece, 0.5mg insect powder 3+0.2mg xylo-oligosaccharide / piece, 0.25mg insect powder Powder 3 + 0.2mg xylo-oligosaccharide / bird, 0.5mg / xylo-oligosaccharide, 0.5mg / distilled water (once a day, add insect powder to 1mL distilled water and feed).
[0042] The feces of the day before the administration and the day after the last administration were aseptically collected, diluted with normal saline, and inoculated on each culture medium with appropriate dilu...
Embodiment 3
[0049] Example 3 Mice intestinal propulsion experiment
[0050] The mouse species was C57BL / 6, purchased from Shanghai Kaisheng Biotechnology Co., Ltd.
[0051] Take 30 healthy male mice with 20g ± 2g diet and good activity status, and divide them into 3 groups on average; on the 1st to 7th day, respectively, each time 0.5mg / mouse (add 1ml of water for intragastric administration) twice a day Feed with distilled water, insect meal 1 or insect meal 2;
[0052] On the 2nd to 7th days, according to 0.004mg / mouse subcutaneous injection of reserpine to make malnutrition model (refer to the preparation method of mouse spleen deficiency malnutrition model in the domestic prior art);
[0053] On the 8th day, feed 1ml of water containing 10% activated carbon powder, and kill the animal 30 minutes later, take the tissue from the pylorus to the ileocecum, lay the intestinal tube in a straight line, measure the total length of the small intestine and the distance from the pylorus to the ...
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