Preparation method and application of nitrogen-sulfur-doped green fluorescent carbon dots
A green fluorescent, sulfur-doped technology, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-optics, etc., to achieve the effects of good fluorescence stability, high fluorescence intensity, and low cytotoxicity
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Embodiment example 1
[0021] Using taurine as a carbon source, at room temperature, 0.5 g of taurine was dissolved in 10 mL of ethanol, diethylene glycol monobutyl ether, toluene, chloroform, tetraethyl orthosilicate, distilled water, and triethanolamine, and ultrasonically Disperse for 10 minutes, transfer to a high-pressure reactor, keep the temperature at 170°C for 8 hours, then cool naturally, use a freeze dryer to dry, then add alcohol to wash, filter to obtain a solution, and rotary evaporate to obtain nitrogen-sulfur doped green fluorescent carbon Points (N-S / CDs) solid. According to the fluorescence photometer, the carbon dot solution of N-S / CDs prepared with triethanolamine as a solvent appears yellowish brown under natural light. By changing the excitation wavelength, the corresponding emission wavelength will also change correspondingly, resulting in a large red shift. The phenomenon has obvious excitation wavelength dependence. The strongest fluorescence emission peaks of N-S / CDs prepa...
Embodiment example 2
[0023] Using triethanolamine as a solvent and taurine as a carbon source, at room temperature, dissolve 0.5 g of taurine in 10 mL of triethanolamine, ultrasonically disperse for 10 min, transfer to an autoclave, and keep the temperature at 160 °C for 10 h, then Cool naturally, dry with a freeze dryer, add alcohol to wash, filter to obtain a solution, and rotary evaporate to obtain nitrogen-sulfur doped green fluorescent carbon dots (N-S / CDs) solid. Through high-resolution transmission electron microscopy, it can be seen that the prepared fluorescent carbon dots have good dispersion in triethanolamine and no agglomeration phenomenon. The appearance is approximately spherical, and the particle size distribution is relatively uniform. The average particle size is 4.0nm. The lattice fringe structure with a lattice spacing of 0.21nm. This kind of fluorescent carbon dots with small particle size can easily enter cells, so it has a wide application prospect in biological imaging and ...
Embodiment example 3
[0025] Using triethanolamine as a solvent and taurine as a carbon source, at room temperature, dissolve 0.35 g of taurine in 10 mL of triethanolamine, ultrasonically disperse for 10 min, transfer to an autoclave, and keep the temperature at 165 °C for 9 h, then Cool naturally, dry with a freeze dryer, add alcohol to wash, filter to obtain a solution, and rotary evaporate to obtain nitrogen-sulfur doped green fluorescent carbon dots (N-S / CDs) solid. The carbon dot powder cytotoxicity test was carried out, and it was found that when the concentration of N-S / CDs was as high as 150 μg / mL, the cell survival rate was still greater than 90%. The morphology of the cells in group (d) was almost the same, and the cells were basically spindle-shaped, indicating that the N-S / CDs solution had little effect on the morphology of the cells. Combined with the data of cytotoxicity experiments, it is further demonstrated that N-S / CDs have little cytotoxicity, and N-S / CDs are non-toxic at a fairl...
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