Skin injury repair factor composition and preparation method thereof
A technology for repairing factors and skin damage, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of wasting biologically active substances in cells, and achieve the effect of avoiding potential harm and reducing costs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0046] The second aspect of the present invention provides a kind of preparation method of above-mentioned skin damage repair factor composition, and this preparation method comprises the following steps:
[0047] The immune cells are subjected to stress-induced culture, collecting the culture supernatant during the stress-induced culture process, and separating the culture supernatant to obtain the immune cell culture secretion;
[0048] Freezing and thawing the cultured immune cells induced by stress stimulation 2 to 4 times, and separating them to obtain the immune cell lysate;
[0049] The immune cell culture secretion and the immune cell lysate are mixed to obtain a skin damage repair factor composition.
[0050] The preparation method of the skin damage repair factor composition, through inducing stress to stimulate culture, carries out directional regulation, and promotes the directional secretion of active substances required by immune cells for skin repair and regener...
Embodiment 1
[0112] Acquisition and first induction culture of human immune cells with high biosafety:
[0113] According to the clinical trial product standards of immune cells, the large-scale expansion of immune cells in vitro is carried out in the GMP workshop, and the cell culture supernatant is obtained, which specifically includes the following steps:
[0114] Collect more than 50ml of umbilical cord blood and peripheral blood from healthy people, separate the plasma, and keep it for use;
[0115] The remaining cells were separated by density gradient centrifugation of human lymphocyte separation medium, and the mononuclear cells of the buffy coat were collected respectively;
[0116] After washing the buffy coat twice, adjust the cell density to 5×10 6 ~1×10 7 cells / ml, inoculated in lymphocyte serum-free medium containing 1000 U / ml of inducible cytokines IL-2, IL-1a, and interferon-γ respectively; at 37°C, 5% CO2, and saturated humidity The cells were cultured, and the cells we...
Embodiment 2
[0165] Acquisition and first induction culture of human immune cells with high biosafety:
[0166] According to the clinical trial product standards of immune cells, the large-scale expansion of immune cells in vitro is carried out in the GMP workshop, and the cell culture supernatant is obtained, which specifically includes the following steps:
[0167] Collect more than 50ml of umbilical cord blood and peripheral blood from healthy people, separate the plasma, and keep it for use;
[0168] The remaining cells were separated by density gradient centrifugation of human lymphocyte separation medium, and the mononuclear cells of the buffy coat were collected respectively;
[0169] After washing the buffy coat twice, adjust the cell density to 5×10 6 ~1×10 7 cells / ml, inoculated in lymphocyte serum-free medium containing 800 U / ml of inducible cytokines IL-2, IL-1a and interferon-γ respectively; at 37°C, 5% CO 2 Cells were cultured in an environment of saturated humidity, half ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com