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Skin injury repair factor composition and preparation method thereof

A technology for repairing factors and skin damage, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of wasting biologically active substances in cells, and achieve the effect of avoiding potential harm and reducing costs

Pending Publication Date: 2019-10-25
北京京蒙细胞生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, people only pay attention to the production and preparation of leukocytes, immune cells, and mesenchymal stem cells in both experimental research and GMP production. utilization, which greatly wastes the biologically active substances secreted by cells during culture

Method used

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  • Skin injury repair factor composition and preparation method thereof
  • Skin injury repair factor composition and preparation method thereof
  • Skin injury repair factor composition and preparation method thereof

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Experimental program
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Effect test

preparation example Construction

[0046] The second aspect of the present invention provides a kind of preparation method of above-mentioned skin damage repair factor composition, and this preparation method comprises the following steps:

[0047] The immune cells are subjected to stress-induced culture, collecting the culture supernatant during the stress-induced culture process, and separating the culture supernatant to obtain the immune cell culture secretion;

[0048] Freezing and thawing the cultured immune cells induced by stress stimulation 2 to 4 times, and separating them to obtain the immune cell lysate;

[0049] The immune cell culture secretion and the immune cell lysate are mixed to obtain a skin damage repair factor composition.

[0050] The preparation method of the skin damage repair factor composition, through inducing stress to stimulate culture, carries out directional regulation, and promotes the directional secretion of active substances required by immune cells for skin repair and regener...

Embodiment 1

[0112] Acquisition and first induction culture of human immune cells with high biosafety:

[0113] According to the clinical trial product standards of immune cells, the large-scale expansion of immune cells in vitro is carried out in the GMP workshop, and the cell culture supernatant is obtained, which specifically includes the following steps:

[0114] Collect more than 50ml of umbilical cord blood and peripheral blood from healthy people, separate the plasma, and keep it for use;

[0115] The remaining cells were separated by density gradient centrifugation of human lymphocyte separation medium, and the mononuclear cells of the buffy coat were collected respectively;

[0116] After washing the buffy coat twice, adjust the cell density to 5×10 6 ~1×10 7 cells / ml, inoculated in lymphocyte serum-free medium containing 1000 U / ml of inducible cytokines IL-2, IL-1a, and interferon-γ respectively; at 37°C, 5% CO2, and saturated humidity The cells were cultured, and the cells we...

Embodiment 2

[0165] Acquisition and first induction culture of human immune cells with high biosafety:

[0166] According to the clinical trial product standards of immune cells, the large-scale expansion of immune cells in vitro is carried out in the GMP workshop, and the cell culture supernatant is obtained, which specifically includes the following steps:

[0167] Collect more than 50ml of umbilical cord blood and peripheral blood from healthy people, separate the plasma, and keep it for use;

[0168] The remaining cells were separated by density gradient centrifugation of human lymphocyte separation medium, and the mononuclear cells of the buffy coat were collected respectively;

[0169] After washing the buffy coat twice, adjust the cell density to 5×10 6 ~1×10 7 cells / ml, inoculated in lymphocyte serum-free medium containing 800 U / ml of inducible cytokines IL-2, IL-1a and interferon-γ respectively; at 37°C, 5% CO 2 Cells were cultured in an environment of saturated humidity, half ...

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Abstract

The invention discloses a skin injury repair factor composition. The skin injury repair factor composition contains immune cell culture secretion and immune cell lysate. By adopting the skin injury repair factor composition, on one hand, immune cells are subjected to directional secretion regulation and control culture, biological active substances such as multiple growth factors, proteases and small molecule polypeptides which are beneficial to skin injury repair in the cultured secretion are enriched, and interoperability between a cell therapy technology and a skin regeneration repair technology is realized; on the other hand, culture supernatants in the clinical research and production processes of stem cells and immune cells can be fully utilized, potential hazards of the culture supernatants serving as biological wastes to the natural environment and human health are avoided, active ingredients in the culture supernatants are developed and utilized, and the cell production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of cell culture application, and in particular relates to a skin damage repair factor composition and a preparation method thereof. Background technique [0002] The aging mechanism of the skin is that reactive oxygen species (ROS) lead to microcirculation disturbance, slow metabolism, weakened cell proliferation and differentiation, and further cause wrinkles and pigmentation, which are the main causes of skin aging. Bioactive substances such as skin cell regeneration regulators, superoxide dismutase (SOD), and bioactive small peptides are the most effective solutions against ROS. Bioactive substances achieve the regeneration of aging skin through the following pathways: by scavenging oxidative free radicals, repairing damaged cells, proliferating collagen and elastic fibers, strengthening the internal support structure of the skin, and activating the regeneration and differentiation of basal stem cells. H...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61Q19/08A61Q19/00
CPCA61K8/99A61K8/64A61Q19/08A61Q19/00A61K2800/805
Inventor 王云虹丁炜李志刚阮志王颖颖杜颖刘湖万兴中
Owner 北京京蒙细胞生物科技股份有限公司
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