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Preparation method and application of human endometrial stem cell compound repair factor

An endometrium and composite repair technology, applied in the biological field, can solve the problems of women's distress, difficulty in endometrial repair, and long treatment time, and achieve strong transdermal absorption, significant therapeutic effect, and long storage time.

Pending Publication Date: 2019-10-29
杨永利
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing private treatment technology is difficult to repair the endometrium, and the treatment time is long. The treatment method of diseased tissue or organ resection is often used, which has brought great troubles to many women.

Method used

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  • Preparation method and application of human endometrial stem cell compound repair factor
  • Preparation method and application of human endometrial stem cell compound repair factor
  • Preparation method and application of human endometrial stem cell compound repair factor

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Embodiment Construction

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0034] see Figure 1~3 , the present invention provides a technical solution: a preparation method of human endometrial stem cell composite repair factor, specifically comprising the following steps:

[0035] S1. Human endometrial stem cells were isolated from human endometrial tissue;

[0036] S2. Human endometrial stem cells were separated by in vitro digestion and expanded culture to obtain high-quality human endometrial stem cells;

[0037] Wherein, the ...

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Abstract

The invention discloses a preparation method and application of a human endometrial stem cell compound repair factor. The preparation method specifically includes the following steps that a human endometrial stem cell is separated from human endometrial tissue; in-vitro digestion separation and expanded culture is performed on the human endometrial stem cell to obtain a high-quality human endometrial stem cell; industrialized amplification culture is performed on the high-quality human endometrial stem cell, and stem cell culture supernatants of the P3-P6 generations are collected; the stem cell culture supernatants are subjected to concentration, protein content determination and quality inspection to obtain a human endometrial stem cell concentration factor; the human endometrial stem cell concentration factor is mixed with a freeze-drying protective agent, and then freeze-drying is conducted to obtain human endometrial stem cell freeze-dried powder; and the human endometrial stem cell freeze-dried powder is mixed with a solvent to obtain the human endometrial stem cell compound repair factor. The repair factor has a remarkable treatment effect, a patient is free of pain, an operation and a surgery are not required, no side effect is caused, and the factor is safe and efficient.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method and application of a human endometrial stem cell composite repair factor. Background technique [0002] The uterus is crucial to a woman's life, and the human endometrium is one of the most active tissues in the body's metabolism and self-renewal. However, once the endometrium is damaged, it will cause a series of consequences. Due to abortion or gynecological inflammation, the endometrium is damaged, and the serious endometrial injury will directly cause the physiological repair obstacle of the endometrium. Eventually lead to habitual abortion, intrauterine adhesions, amenorrhea and even infertility and other complications. Endometrial injury can also lead to cervicitis, endometritis, endometrial polyps, endometrial injury, uterine fibroids and precancerous lesions. [0003] Endometrial Derived Stem Cells (EDSCs) are undifferentiated endometrial cells with se...

Claims

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Application Information

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IPC IPC(8): A61K35/545A61K9/08A61P15/00A61P15/08A61P29/00A61P35/00C12N5/074
CPCA61K35/545A61K9/08A61K9/0034A61P15/00A61P15/08A61P35/00A61P29/00C12N5/0682C12N2509/00
Inventor 杨永利
Owner 杨永利
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