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Method for extracting protein from grape seeds

A grape seed and protein technology is applied in the field of protein extraction to achieve the beneficial effects of improving the extraction rate and shortening the time.

Active Publication Date: 2019-11-08
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the main methods of extracting grape seed protein are cloud point extraction method, reverse micellar extraction method, acid-soluble alkali precipitation method, precipitation method, electrophoresis method, and the combination of high-voltage pulsed electric field technology and cloud point extraction technology to extract grape seeds. method has not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Raw material pretreatment: After soaking grape seeds in distilled water for 30 hours, carry out Buhler test milling at a feeding speed of 90g / min, after passing through a 100-mesh sieve, add petroleum ether according to the solid-to-liquid ratio of 1:8, and stir at room temperature 3h, filter with suction, place the obtained precipitate in a ventilated place, and collect the grape seed powder for later use after the petroleum ether has volatilized completely;

[0028] 2) Preparation of extractant: add polyoxyethylene octylphenol ether and dodecyl polyethylene glycol ether to deionized water in a ratio of 2:1 to form a 0.05g / mL solution, and stir with a magnetic stirrer 2h, make it mix evenly, then add KCl solution, keep the content of KCl solution at 1.5% (w / v) and stir for 15min;

[0029] 3) Grape seed powder mixed with extractant: weigh 40 g of grape seed powder, add 720 mL of the extractant prepared in step 2), maintain a solid-liquid ratio of 1:18, and stir magne...

Embodiment 2

[0035] 1) Raw material pretreatment: After soaking grape seeds in distilled water for 32 hours, carry out Buhler test milling at a feeding rate of 100g / min, after passing through a 90 mesh sieve, add petroleum ether according to the solid-to-liquid ratio of 1:10, and stir at room temperature After 4 hours, filter with suction, place the obtained precipitate in a ventilated place, and collect the grape seed powder for later use after the petroleum ether has volatilized completely;

[0036]2) Preparation of extractant: Add polyoxyethylene octylphenol ether and dodecyl polyethylene glycol ether in a ratio of 2.5:1 to deionized water to form a 0.05g / mL solution, and stir with a magnetic stirrer 2h, make it mix evenly, then add KCl solution, keep the content of KCl solution at 1.4% (w / v) and stir for 20min;

[0037] 3) Grape seed powder mixed with extractant: weigh 60 g of grape seed powder, add 1200 mL of the extractant prepared in step 2), maintain a solid-liquid ratio of 1:20, a...

Embodiment 3

[0043] 1) Raw material pretreatment: after soaking grape seeds in distilled water for 31 hours, carry out Buhler test milling at a feeding speed of 110g / min, after passing through a 120 mesh sieve, add petroleum ether according to the solid-to-liquid ratio of 1:9, and stir at room temperature After 3.5 hours, filter with suction, and place the obtained precipitate in a ventilated place. After the petroleum ether has evaporated, collect the grape seed powder for later use;

[0044] 2) Preparation of extractant: Add polyoxyethylene octylphenol ether and dodecyl polyethylene glycol ether in a ratio of 2.6:1 to deionized water to form a 0.05g / mL solution, and stir with a magnetic stirrer 2.5h, make it mix evenly, then add KCl solution, keep the content of KCl solution at 1.5% (w / v) and stir for 20min;

[0045] 3) Grape seed powder is mixed with the extractant: weigh 45 g of grape seed powder, add 900 mL of the extractant prepared in step 2), maintain a solid-liquid ratio of 1:20, ...

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Abstract

The invention relates to the field of protein extraction, and discloses a method for extracting protein from grape seeds. According to the method for extracting the protein from the grape seeds, a Buller test mill is adopted to smash the grape seeds, a high-voltage pulsed electric field extraction method is combined with a cloud point extraction technology, and the protein is extracted from the grape seeds. Non-ionic surface active agents are adopted, compared with a charged ionic surface active agent, the influence of the non-ionic surface active agents on protein denuturation is small. Two non-ionic surface active agents of polyoxyethylene octyl phenol ether and dodecyl polyethylene glycol ether are mixed, so that a cloud point of a mixing system is reduced, and phase separation is promoted to improve the extraction efficiency. Addition of KC1 can enable a cloud point of a micellar system to be reduced due to a salting-out effect, inorganic salt can reduce repulsion between micelles,attraction is increased, the micelles are precipitated, meanwhile, the solvent polarity can be increased through addition of the KC1, and thus an extraction agent is further suitable for a high-voltage pulse technology under the condition of meeting cloud point extraction.

Description

technical field [0001] The invention relates to the field of protein extraction, in particular to a method for extracting protein from grape seeds. Background technique [0002] The annual production of grapes in the world is about 65 million tons, 80% of which are used for winemaking, and a large amount of discarded grape seeds are produced while making wine. At present, the research on grape seeds is mainly focused on the processing and utilization of grape seed oil, and there is little research on the protein of grape seeds. The protein in grape seeds is a good protein resource, and grape seed protein contains high-quality crude protein , the polypeptide formed after hydrolysis is easily digested and absorbed by the human body. In addition, it also contains 8 kinds of amino acids necessary for the human body. The content of some amino acids is comparable to that of soybeans. Adding it to food can effectively improve the nutritional value of the product. Grape seed protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/30
CPCC07K1/145C07K1/30
Inventor 彭志勤王晓云刘珉琦黄诗莹万军民王秉
Owner ZHEJIANG SCI-TECH UNIV