Method of promoting bacillus spore germination

A technology of Bacillus and Bacillus, applied in the field of rhizobacteria, which can solve the problems of insufficient understanding of the mechanism and chemical signals of bacterial spore germination and vegetative growth

Pending Publication Date: 2019-11-15
BAYER CROPSCI LP
View PDF13 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanisms and chemical signals that promote bacterial spore germination and vegetative growth are not well understood

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of promoting bacillus spore germination
  • Method of promoting bacillus spore germination
  • Method of promoting bacillus spore germination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1. (Bacillus subtilis strain QST713) and triple superphosphate effect evaluation on potato

[0109] A field trial was conducted in Hatay Province, Turkey to evaluate the (Bacillus subtilis strain QST713) applied to potatoes grown according to standard local practice. Whole potatoes were treated with triple superphosphate (also known as TSP and Ca(H 2 PO 4 ) 2 ·H 2 O) Processing. Apply at 10 l / ha (Bacillus subtilis strain QST713), TSP was applied at 600 kg / ha. (Bacillus subtilis strain QST713) and TSP were both applied at planting. Four replicates of each treatment were included in the field trial.

[0110] At harvest, tuber weights were measured for each replicate. Compared with potatoes treated with TSP alone, with TSP and (Bacillus subtilis strain QST713) treated potato tubers had an average increase in weight of 19%, corresponding to an average increase of 13.8 grams per tuber (see Table 1).

[0111] A similar increase in yield was observed. ...

Embodiment 2

[0121] Example 2. Confirmation (Bacillus subtilis strain QST713) and triple superphosphate on potato yield

[0122] Additional field trials were conducted in the Turkish cities of Ankara, Izmir and Adana to confirm the (Bacillus subtilis strain QST713) and TSP applied to the yield effect of potatoes grown according to standard local practice. Apply at the time of planting at the same rate as described in Example 1 (Bacillus subtilis strain QST713) and TSP.

[0123] At harvest, potato yield was assessed in each field trial. In all three experiments, compared with potatoes treated with TSP alone, treatment with TSP and (Bacillus subtilis strain QST713) treated potatoes had higher yields (see Table 4). These results confirm that, (Bacillus subtilis strain QST713) combined application with inorganic phosphate provided additional yield.

[0124] Table 4. Administration of TSP alone or administration of TSP and Potato yield (kg / da) after (Bacillus subtilis strain QST71...

Embodiment 3

[0126] Embodiment 3. Potassium dihydrogen phosphate is to the germination of Bacillus subtilis QST713 bacterial spore and the effect evaluation of growth

[0127] To determine the effect of inorganic phosphate on the germination and vegetative growth of Bacillus spores, purified spore preparations of Bacillus subtilis QST713 were grown in minimal medium containing or 12.5mM potassium dihydrogen phosphate (KH 2 PO 4 ), and compared with a control that did not contain potassium dihydrogen phosphate. use Cell Viability Reagent (Invitrogen, Carlsbad, California) monitored the metabolic activity of germinating and actively growing bacterial spores. metabolically active cells Cell viability reagents are reduced and produce fluorescent chemical products that provide a quantitative measure of growing cells. Potassium dihydrogen phosphate does not stimulate germination or growth of Bacillus sp. QST713 spores in the absence of growth nutrients (i.e., no carbon source) (see figur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides a method of promoting spore germination and / or vegetative growth of a plant growth-promoting Bacillus or Paenibacillus isolate, the method comprising simultaneously or sequentially applying inorganic phosphate and the plant growth-promoting Bacillus or Paenibacillus isolate to a plant, a plant part, a plant propagule, a seed of a plant and / or a locus where a plant isgrowing or is intended to grow.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to U.S. Provisional Patent Application No. 62 / 450,735, filed January 26, 2017, the contents of which are incorporated herein by reference in their entirety. technical field [0003] The present application relates to the field of rhizobacteria that promote plant growth, and in particular to methods of promoting spore germination and / or vegetative growth of Bacillus isolates by applying inorganic phosphates and Bacillus isolates. Background technique [0004] Certain microorganisms, such as various Bacillus species, will form endospores in response to environmental factors. Spores are rigid structures with no detectable metabolic activity. The general spore structure includes the outer exosporium, spore coat, spore cortex, inner membrane and finally the spore core cell. Compared to vegetative cells, endospores are significantly more resistant to environmental fluctuations in pH, temper...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A01N63/00C05B17/00C05F11/08C12R1/07C12R1/01A01N63/20A01N63/22
CPCC05B17/00C05F11/08A01N63/22A01N63/20C12R2001/07C12N1/205C05B1/04C12R2001/125
Inventor A·希尔林V·P·托马斯周鳳玲
Owner BAYER CROPSCI LP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap