Hybridoma cell strain, getah virus distinguishing monoclonal antibody secreted from hybridoma cell strain, and application

A hybridoma cell line and monoclonal antibody technology, which is applied in the field of bioengineering, can solve the problems of preparation and application of GETV monoclonal antibody not seen in Gaita virus, and achieve the effects of wide application space, strong specificity and high sensitivity
CN110484510AActive Publication Date: 2019-11-22HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Publication Date
2019-11-22

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Abstract

The invention discloses a hybridoma cell strain, a getah virus distinguishing monoclonal antibody secreted from the hybridoma cell strain, and an application. The hybridoma cell strain is a K3A6B6 hybridoma cell strain secreting the monoclonal antibody for distinguishing getah virus E2 protein. The monoclonal antibody secreted from the hybridoma cell strain can be specially bound with target antigen in high titer, and the monoclonal antibody is prepared into a detection clinical sample for detecting a reagent kit. Specificity is high, sensitivity is high, and stability is good.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering. The invention relates to a hybridoma cell line, anti-Gaitavirus monoclonal antibody secreted by the hybridoma cell line and its application. Background technique

[0002] Getah virus (GETV) is a member of the genus Alphavirus in the family Togaviridae. The size of the virus particle is about 70nm, spherical structure, with capsule and fiber, the virus genome is a single-stranded positive-strand RNA, and the genome has two open reading frames (ORF), which encode two polyproteins, and are finally cut into 4 There are three nonstructural proteins (NSP1, NSP2, NSP3 and NSP4) and five structural proteins (C, E3, E2, 6K and E1).

[0003] In 1955, Scherer et al first isolated the virus from Culex spp.mosquitoes in Malaysia. Serological test results show that GETV is widely distributed in Europe, Asia and Oceania, and GETV antibodies have been detected in various animals such as pigs, horses, c...

Claims

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