Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glow-type chemiluminescent sensor based on hydrogel and its preparation method and application

A chemiluminescence and hydrogel technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve problems such as limited application, complex electrode modification process equipment and equipment, etc., to enhance chemiluminescence intensity , The effect of long chemiluminescence time and high sensitivity

Active Publication Date: 2021-09-07
SOUTHEAST UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods have complicated electrode modification process and complex instruments and equipment, which limit the application of these methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glow-type chemiluminescent sensor based on hydrogel and its preparation method and application
  • Glow-type chemiluminescent sensor based on hydrogel and its preparation method and application
  • Glow-type chemiluminescent sensor based on hydrogel and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 ABEI / Co 2+ Synthesis of / CS hydrogel

[0050] In 15mL alkaline solution (mass ratio is LiOH: KOH: urea: H 2 (O=4.5:7:8:80.5) stirring and dispersing 0.4g chitosan powder, adding 0.6mL CoCl 2 (1mM) solution, stirred for 5 minutes, and refrigerated at -20°C until completely frozen. After thawing at room temperature, 1.5 mL of ABEI (10 mM) solution was added and stirred for 5 hours to uniformly disperse in the system to obtain ABEI / Co 2+ / CS hydrogel and store at 4 °C.

[0051] figure 2 A shows the synthetic ABEI / Co 2+ / CS SEM image of the hydrogel; the characteristic absorption peak of ABEI is located at the red line at 291nm, and the ultraviolet-visible absorption spectrum proves that ABEI is successfully wrapped in the hydrogel.

Embodiment 2

[0052] Example 2 ABEI / Co 2+ Synthesis of / CS hydrogel

[0053] In 15mL alkaline solution (mass ratio is LiOH: KOH: urea: H 2 (O=4.5:7:8:80.5) stirring and dispersing 0.4g chitosan powder, adding 0.6mL CoCl 2 (0.1 mM) solution, stirred for 5 minutes, and refrigerated at -20°C until completely frozen. After thawing at room temperature, add 1.5mL ABEI (4mM) solution and stir for 5 hours to be uniformly dispersed in the system to obtain ABEI / Co 2+ / CS hydrogel and store at 4 °C.

Embodiment 3

[0054] Example 3 Synthesis of MOF-Pt

[0055] First, weigh 0.2g of TCPP, 0.12g of AlCl 3 ·3H 2 The CTAB of O and 0.144g was dissolved in 20mL water, stirred for 10 minutes and placed in a 20mL reactor, then reacted at 180°C for 16 hours, after centrifuging at 3000rpm, the supernatant was centrifuged at 13000rpm to obtain a precipitate. The precipitate was washed twice with DMF, water and acetone, respectively, and then dried overnight in vacuum. Then, purple and black precipitates were obtained by vacuum drying at 150°C for 7 hours for further reaction. Then weigh 0.038g of precipitate and 0.064g of Cu(Ac) 2 It was dissolved in 8ml of DMF organic solvent, stirred for 10 minutes, then poured into a 20mL reaction kettle, and reacted at 100°C for 24 hours to obtain a red-black blocky precipitate. After centrifugation at 13000rpm, the precipitate was washed twice with DMF, water and acetone, and then dried in vacuo overnight to obtain product 1.

[0056] Finally, 0.5ml of H ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
volumeaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a hydrogel-based glow-type chemiluminescent sensor, a preparation method and an application thereof. The invention also discloses an analysis method for detecting organophosphorus pesticides with a glow-type chemiluminescent sensor constructed on the basis of hydrogel, which includes the following steps: 1) preparing ABEI / Co 2+ / CS hydrogel; 2) Preparation of MOF-Pt material; 3) Mixing reaction of organophosphorus pesticide and acetylcholinesterase to obtain a mixed solution, and then adding acetylcholine chloride solution, choline oxidase solution and PBS buffer to the mixed solution The reaction mixture is obtained, MOF-Pt and the reaction mixture are added to the hydrogel, and the chemiluminescence intensity is measured with a chemiluminescence imager. The present invention does not require sophisticated and expensive experimental instruments, does not have strict and complicated experimental operation procedures, greatly reduces the detection cost of organophosphorus pesticides, and has the advantages of high sensitivity, short detection time, long luminescence time, and high-throughput detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a glow-type chemiluminescent sensor constructed on the basis of hydrogel and its preparation method and application. Background technique [0002] Pesticides are the main substances used in the modern agricultural industry to prevent, regulate or kill other organisms that harm crop growth. Organophosphorus pesticides (OPs) have been widely used in agriculture and forestry because of their high insecticidal efficiency, high crop yield, and easy degradation. However, most organophosphate pesticides are highly toxic. Excessive or improper use will cause environmental pollution. Because OPS can inhibit the activity of acetylcholinesterase (AChE), the central nervous system is seriously disturbed, causing harm to human health, and even causing death in severe cases. Therefore, the rapid and sensitive detection of OPS is of great significance to life health and environmental protection....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76C08J3/075C08L5/08C08K3/16C08K5/24
CPCC08J3/075C08J2305/08C08K3/16C08K5/24G01N21/76
Inventor 卫伟陆叶卫敏刘松琴
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com