Heat-clearing eight-ingredient pill detection method

A detection method and a technology of a determination method, which are applied in the quality detection of Mongolian medicine and the quality detection field of Mongolian medicine product Qingre Bawei Pills, to achieve the effect of product quality safety and sensitivity improvement

Inactive Publication Date: 2019-11-22
INNER MONGOLIA MONGOLIAN MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current quality standards include traits, microscopic identification, physical and chemical identification, inspection, and the determination of the content of artificial bezoar by th

Method used

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  • Heat-clearing eight-ingredient pill detection method
  • Heat-clearing eight-ingredient pill detection method
  • Heat-clearing eight-ingredient pill detection method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0076] The identification of embodiment 1 Huanglian:

[0077] Preparation of the test solution: take 3 g of this product, grind it finely, add 15 ml of 10% sulfuric acid solution, heat to reflux for 15 min, let it cool, extract twice with chloroform, combine the chloroform, and concentrate to 2 ml as the supply. Test solution.

[0078] Preparation of reference medicinal material solution: take 1 g of Coptis chinensis reference medicinal material, add 15 ml of 10% sulfuric acid solution, heat under reflux for 15 min, let cool, extract twice with chloroform, combine with chloroform, and concentrate to 2 ml as a reference medicinal solution.

[0079] Preparation of reference substance solution: take appropriate amount of vanillic acid reference substance and cinnamic acid reference substance, add chloroform respectively to make a solution containing 2mg per 1ml, as reference substance solution.

[0080] Identification: According to the thin-layer chromatography test, draw 10 μl ...

Example Embodiment

[0081] The identification of embodiment 2 Kuditin:

[0082] Preparation of the test solution: take 3g of this product, grind it finely, add 1ml of concentrated ammonia water to infiltrate, add 20ml of chloroform, leave it for 10h, filter, evaporate the filtrate to dryness, add 1ml of methanol to the residue to dissolve, as the test sample solution.

[0083] Preparation of reference medicinal material solution: take 1 g of Kuditin reference medicinal material, infiltrate with 1 ml of concentrated ammonia water, add 20 ml of chloroform, leave for 10 hours, filter, evaporate the filtrate to dryness, add 1 ml of methanol to the residue to dissolve, and serve as a reference medicinal material solution.

[0084] Identification: According to the thin-layer chromatography test, draw 10 μl of the above test solution and reference medicinal material solution, respectively, point them on the same silica gel G thin-layer plate, and use 7:2:1 cyclohexane-trichloromethane-methanol as the de...

Example Embodiment

[0085] Embodiment 3 Determination of hydroxysafflor yellow A content

[0086] HPLC conditions: use octadecylsilane-bonded silica gel as filler; use 9:1:40 methanol-acetonitrile-0.7% phosphoric acid as mobile phase; detection wavelength is 403 nm; flow rate is 1 ml / min; column temperature is 35 ℃, the injection volume is 10 μl.

[0087] Preparation of the test solution: take an appropriate amount of this product, grind it finely, take 2g, accurately weigh it, put it in a 50ml volumetric flask, add an appropriate amount of 25% methanol solution, ultrasonic power 300W, frequency 50kHz for 40min, let cool to room temperature, add The 25% methanol solution was adjusted to the mark, shaken well, passed through a 0.45 μm microporous membrane, and the subsequent filtrate was obtained.

[0088] Preparation of reference solution: take an appropriate amount of hydroxysafflor yellow A reference substance, accurately weigh it, and add 25% methanol aqueous solution to make a solution conta...

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Abstract

The invention provides a heat-clearing eight-ingredient pill detection method, which belongs to the field of medicine analysis. The existing quality standard of heat-clearing eight-ingredient pills iscorrespondingly improved, and a method of distinguishing Hu Huanglian and Kudingding with strong specificity is added on the basis of the original quality standard, and a method of measuring the content of hydroxysafflor yellow A is added. The method has the characteristics of simplicity, fast speed, good reproducibility and the like. The established identification and content measurement methodsmeet relevant requirements through methodological verification, have strong applicability, can more comprehensively reflect the product quality status and provide an important basis for quality control and standard setting.

Description

technical field [0001] The invention relates to a quality detection method of Mongolian medicine, in particular to a quality detection method of Mongolian medicine product Qingre Bawei Pill, which belongs to the technical field of medicine analysis. Background technique [0002] Qingre Bawei Pills, as a Mongolian medicine product with national drug quality standards, are prepared from sandalwood, gypsum, safflower, kudiding, Qumai, Huhuanglian, Ophiopogon japonicus, and artificial bezoar. It has the effect of clearing away heat and detoxifying. For red hot, blood heat, heat of viscera, cough due to lung heat, bloody phlegm, liver fire and pain in the ribs. This drug has been used clinically for many years and is a national essential drug. It has achieved relatively good therapeutic effects in clearing away heat and detoxifying. [0003] The current quality standards include traits, microscopic identification, physical and chemical identification, inspection, and determinati...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N30/90
Inventor 邢界红刘庆玲龙泉洪于秀玲
Owner INNER MONGOLIA MONGOLIAN MEDICINE CO LTD
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