A three-dimensional tissue model with double network structure and its perfusion integrated preparation method

Abstract

The invention discloses a three-dimensional tissue model with a double network structure and a perfusion integrated preparation method thereof. The three-dimensional tissue model includes a tissue matrix, a horizontal large-diameter channel array network penetrating along the horizontal direction of the tissue matrix, and a channel array network penetrating along the vertical direction of the tissue matrix. The vertical small-diameter scale channel array network; the horizontal large-diameter scale channel array network and the vertical small-diameter scale channel array network are interpenetrated and combined, and are respectively connected to two sets of independent external perfusion culture systems, combining convection and osmosis. The method uses two sets of networks to perform filtering perfusion culture on the tissue model; the invention also discloses a method for the simple and rapid manufacture of the three-dimensional tissue model and the integration of subsequent perfusion culture; the three-dimensional tissue with double network structure can be used for pharmacological and physiological exploration. Provide research models, including drug screening models, tumor tissue models, organoid models, vascular-matrix interaction models, etc.

Description

technical field [0001] The invention relates to the technical field of biomanufacturing and tissue engineering, in particular to a three-dimensional tissue model with a double network structure and a perfusion integrated preparation method thereof. Background technique [0002] A dynamic network of vascular channels of various calibers plays an integral role in maintaining homeostasis. Especially in organs or tissues with high cell density and vigorous metabolism, such as liver and brain, the vascular network ensures the transportation of nutrients and the removal of metabolic waste, so that it can maintain cell vitality and organ function for a long time. In addition to this, the vascular network also functions as a conduit for the local and systemic distribution of cell signaling molecules within tissues. [0003] The use of in vitro tissue engineering to construct organoid tissue models has experienced the development process from two-dimensional plane culture represente...

AI Technical Summary

Problems solved by technology

In three-dimensional tissue static or dynamic perfusion culture, for tissues that do not contain a rich vascularized channel network, there may be insufficient transport of nutrients or oxygen to the interior of the tissue and the failure of cell metabolites to be discharged in time, resulting in tissue necrosis in the heart, and long-term growth cannot be achieved. training problem

However, the channel density of the three-dimensional tissue prepared by the current method is limited, and the direction of the mass transfer channel is a single direction. Nutrient transport and metabolite excretion share a set of networks. The effect of mass transfer, especially the discharge of metabolic waste, is limited. Cells maintain long-term high-quality survival and growth, and the beneficial effects of differentiation and proliferation are limited

In addition, the existing preparation methods have problems such as complicated technical process, separation of the preparation system and the perfusion culture system, and the transfer of three-dimensional tissues in the whole process will increase the risk of tissue damage and contamination

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