Straw biodegradation complex bacterial agent and application thereof

A compound bacterial agent and straw biological technology, which is applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of reducing the market competitiveness of enterprise products, polluting the environment, increasing the production cost of enterprises, and avoiding chemical The use of preparations, the high degree of industrialization, and the effect of reducing energy consumption

Inactive Publication Date: 2019-12-17
GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
View PDF10 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pulping is the first process in the papermaking industry. At present, chemical pulping or chemical mechanical pulping methods are basically used in the world, mainly alkali method, ammonium sulfite method, etc. Therefore, the traditional pulping process is To a certain extent, it will be accompanied by the discharge of a large amount of black liquor waste, which seriously pollutes the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Straw biodegradation complex bacterial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] An embodiment of the straw biodegradable composite bacterial agent of the present invention, the preparation method of the straw biodegradable composite bacterial agent described in this embodiment is as follows:

[0048] 1) Incline cultivation: Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus were inoculated on YPD plates for activation for 3 days; Phanerochaete chrysosporium and Trametes versicolor were inoculated on potato medium (PDA) plates for activation for 4 days.

[0049] 2) Inoculate the above-mentioned activated strains in the corresponding liquid medium under aseptic conditions, and cultivate them on a shaker at 30° C. and 180 rpm. Among them, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus cereus were cultured for 2 days; Phanerochaete chrysosporium and Trametes versicolor were cultured for 5 days;

[0050] 3) Inoculate the above-mentioned seed solution into a liquid medium, and ferment and cultivate. The inoculum size is 1% of t...

Embodiment 2

[0063] An embodiment of the straw biodegradable composite bacterial agent of the present invention, the preparation method of the straw biodegradable composite bacterial agent described in this embodiment is as follows:

[0064] 1) Incline cultivation: Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus were inoculated on YPD plates for activation for 1 day; Phanerochaete chrysosporium and Trametes versicolor were inoculated on potato medium (PDA) plates for activation for 3 days.

[0065] 2) Inoculate the above-mentioned activated strains in the corresponding liquid medium under aseptic conditions, and cultivate them on a shaker at 30° C. and 180 rpm. Among them, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus cereus were cultured for 3 days; Phanerochaete chrysosporium and Trametes versicolor were cultured for 3 days;

[0066] 3) Inoculate the above-mentioned seed solution into a liquid medium, and ferment and cultivate. The inoculum size is 0.5% o...

Embodiment 3

[0079] An embodiment of the straw biodegradable composite bacterial agent of the present invention, the preparation method of the straw biodegradable composite bacterial agent described in this embodiment is as follows:

[0080] 1) Slope culture: inoculate Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus on YPD plates for activation for 2 days; Phanerochaete chrysosporium and Trametes versicolor are inoculated on potato medium (PDA) plates for activation for 5 days.

[0081] 2) Inoculate the above-mentioned activated strains in the corresponding liquid medium under aseptic conditions, and cultivate them on a shaker at 30° C. and 180 rpm. Among them, Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus were cultured for 4 days; Phanerochaete chrysosporium and Trametes versicolor were cultured for 4 days;

[0082] 3) Inoculate the above-mentioned seed solution into a liquid medium, and ferment and cultivate. The inoculum size is 2% of the volume ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Ring pressure indexaaaaaaaaaa
Login to view more

Abstract

The invention discloses a straw biodegradation complex bacterial agent. The complex bacterial agent is a compound of bacillus subtilis, bacillus amyloliquefaciens, bacillus cereus, phanerochaete chrysosporium and trametes versicolor. The invention belongs to the environmental-protection technology, a chemical pulping method commonly used in the current pulping technology is replaced by microbial degradation of plant straw, thus the environmental pollution caused by the use of a large number of chemical reagents in the traditional pulping process is avoided, in the production process of pulping, water can be recycled without discharging any sewage containing organic compounds and suspended solids, the link of sewage treatment is avoided, the production cost is saved, in the whole productionprocess, heating is not needed, decomposing is conducted at normal temperature, and bleaching is conducted at normal temperature. Meanwhile, the invention further discloses a preparation method and application of the straw biodegradation complex bacterial agent.

Description

technical field [0001] The invention relates to the technical field of straw degradation, in particular to a straw biodegradation composite microbial agent and its application. Background technique [0002] Straw cellulose is a very valuable biomass energy resource on the earth, which widely exists in various agricultural production systems. As a large grain-producing country, my country produces nearly 700 million tons of crop straw every year according to statistics. However, due to the lack of guidance and effective treatment methods, the problem of straw disposal has become increasingly prominent. Most of the disposal methods for straw are burning or discarding. This has also become a new source of rural environmental pollution. The current treatment of straw not only wastes precious renewable resources, but also brings various hazards, causing air pollution, soil mineralization, fires and traffic accidents. How to comprehensively and effectively use crop straw to avoid ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N1/14D21C5/00D21B1/16C12R1/125C12R1/07C12R1/085C12R1/645
CPCC12N1/14C12N1/20D21B1/16D21C5/005
Inventor 周辉
Owner GUANGDONG PROPHET BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap