Straw biodegradation complex bacterial agent and application thereof
A compound bacterial agent and straw biological technology, which is applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of reducing the market competitiveness of enterprise products, polluting the environment, increasing the production cost of enterprises, and avoiding chemical The use of preparations, the high degree of industrialization, and the effect of reducing energy consumption
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Embodiment 1
[0047] An embodiment of the straw biodegradable composite bacterial agent of the present invention, the preparation method of the straw biodegradable composite bacterial agent described in this embodiment is as follows:
[0048] 1) Incline cultivation: Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus were inoculated on YPD plates for activation for 3 days; Phanerochaete chrysosporium and Trametes versicolor were inoculated on potato medium (PDA) plates for activation for 4 days.
[0049] 2) Inoculate the above-mentioned activated strains in the corresponding liquid medium under aseptic conditions, and cultivate them on a shaker at 30° C. and 180 rpm. Among them, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus cereus were cultured for 2 days; Phanerochaete chrysosporium and Trametes versicolor were cultured for 5 days;
[0050] 3) Inoculate the above-mentioned seed solution into a liquid medium, and ferment and cultivate. The inoculum size is 1% of t...
Embodiment 2
[0063] An embodiment of the straw biodegradable composite bacterial agent of the present invention, the preparation method of the straw biodegradable composite bacterial agent described in this embodiment is as follows:
[0064] 1) Incline cultivation: Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus were inoculated on YPD plates for activation for 1 day; Phanerochaete chrysosporium and Trametes versicolor were inoculated on potato medium (PDA) plates for activation for 3 days.
[0065] 2) Inoculate the above-mentioned activated strains in the corresponding liquid medium under aseptic conditions, and cultivate them on a shaker at 30° C. and 180 rpm. Among them, Bacillus subtilis, Bacillus amyloliquefaciens and Bacillus cereus were cultured for 3 days; Phanerochaete chrysosporium and Trametes versicolor were cultured for 3 days;
[0066] 3) Inoculate the above-mentioned seed solution into a liquid medium, and ferment and cultivate. The inoculum size is 0.5% o...
Embodiment 3
[0079] An embodiment of the straw biodegradable composite bacterial agent of the present invention, the preparation method of the straw biodegradable composite bacterial agent described in this embodiment is as follows:
[0080] 1) Slope culture: inoculate Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus on YPD plates for activation for 2 days; Phanerochaete chrysosporium and Trametes versicolor are inoculated on potato medium (PDA) plates for activation for 5 days.
[0081] 2) Inoculate the above-mentioned activated strains in the corresponding liquid medium under aseptic conditions, and cultivate them on a shaker at 30° C. and 180 rpm. Among them, Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus were cultured for 4 days; Phanerochaete chrysosporium and Trametes versicolor were cultured for 4 days;
[0082] 3) Inoculate the above-mentioned seed solution into a liquid medium, and ferment and cultivate. The inoculum size is 2% of the volume ...
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