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Sesquiterpene synthases for the production of butanol and mixtures thereof

A technology of tonicol and mixture, applied in the directions of biochemical equipment and methods, enzymes, lyases, etc., can solve problems such as non-cost-effectiveness and complexity

Pending Publication Date: 2019-12-17
FIRMENICH SA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical synthesis routes have been developed, but are still complex and not cost-effective

Method used

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  • Sesquiterpene synthases for the production of butanol and mixtures thereof
  • Sesquiterpene synthases for the production of butanol and mixtures thereof
  • Sesquiterpene synthases for the production of butanol and mixtures thereof

Examples

Experimental program
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Effect test

Embodiment approach

[0087] a. Contacting an acyclic farnesyl diphosphate (FPP) precursor with a polypeptide having sesquiterpene synthase activity to produce bumanol or a mixture comprising bumanol and one or more terpenes, wherein The polypeptide comprises at least 50%, 55%, 60%, 65%, 70%, 75%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, an amino acid sequence with 98% or 99% sequence identity, or an amino acid sequence comprising SEQ ID NO: 2; and

[0088] b. Optionally, isolating remanol.

[0089] 2. The method of embodiment 1, comprising transforming a host cell or a non-human host organism with a nucleic acid encoding a polypeptide having sulphurol synthase activity, wherein the polypeptide comprises at least 50%, 55%, An amino acid sequence having 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity, or comprising SEQ ID NO:2.

[0090] 3. The method of one of the preceding embodiments, further comprising culturing a non-human host organism or host cell capable of producin...

Embodiment 1

[0196] Narrow-leaved peony (Paeonia anomala) plant material tracing and root transcriptome sequencing.

[0197] Paeonia anomala plant material was obtained from Datong, Qinghai, China. To determine whether Paeonia japonicus contains butanol, the roots were collected and freshly extracted with dichloromethane for chemical analysis. The extract was analyzed by GC-MS, and the parameters of GC-MS analysis were described as follows: using a column equipped with DB1-ms (30m × 0.25mm × 0.25μm film thickness, P / N122-0132 (J&W Scientific Inc, Folsom, CA)) And Agilent 6890 series GC system connected with 5975 series mass spectrometer. The carrier gas was helium at a constant flow rate of 0.7 mL / min. The sample injection adopts the split (1:5) mode, and the temperature of the injector is set at 250 °C. The oven temperature was programmed from 50°C (5 minute hold) to 300°C at a rate of 5°C / minute to 300°C, then to 340°C at a rate of 50°C / minute and held for 3 minutes. Product iden...

Embodiment 2

[0200] Functional expression and characterization of PaTPS3.

[0201] First, the total RNA extracted by the Column Plant RNAout kit was reverse-transcribed into cDNA using the SuperScript III First-Strand Synthesis kit (Invitrogen, Shanghai, China). The product was then used as a template from the narrow leaf Genes amplified from the cDNA library of Paeoniae alba.

[0202] The sequence of PaTPS3 was optimized and synthesized by following the genetic code frequency of Escherichia coli. A restriction site for NdeI was added to the 5' end of PaTPS3, while a restriction site for KpnI was added to the 3' end. PaTPS3 was subcloned into the pJ401 (DNA 2.0) plasmid for subsequent expression in E. coli.

[0203] KRX E. coli cells (Promega) were co-transformed with plasmid pACYC / ScMVA (containing genes encoding the heterologous mevalonate pathway) and plasmid pJ401-PaTPS3. To construct the pACYC / ScMVA plasmid, we divided the 8 biosynthetic genes into 2 synthetic operons, termed th...

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Abstract

The present application relates to a method for producing tomanol and / or tomanol derivatives by contacting at least one polypeptide with farnesyl diphosphate (FPP). The method can be performed in vitro or in vivo. Also provided are amino acid sequences of polypeptides useful in the methods and nucleic acids encoding the polypeptides. The method also provides a host cell or organism that is genetically modified to express the polypeptide and that can be used to produce tonicol and / or tonicol derivatives.

Description

technical field [0001] Provided herein are biochemical methods of producing tomanol and related compounds and derivatives, and mixtures comprising tomanol, which methods include the use of novel polypeptides. Background technique [0002] Terpenes are found in most living organisms (microorganisms, animals and plants). These compounds are composed of five carbon units called isoprene units and are classified by the number of these units present in their structure. Thus, monoterpenes, sesquiterpenes, and diterpenes are terpenes that contain 10, 15, and 20 carbon atoms, respectively. For example, sesquiterpenes are widely found in the plant kingdom. Many sesquiterpene molecules are well known for their flavor and aroma properties as well as for their cosmetic, medical and antibacterial effects. A number of sesquiterpene hydrocarbons and sesquiterpenoids have been identified. [0003] The biosynthetic production of terpenes involves enzymes called terpene synthases. Variou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12N9/16C12P7/04C12N9/10C12N9/88C12P5/00
CPCC12N9/88C12P5/007C12Y402/03C12P7/02C12Y402/03126
Inventor 李攀王祺何绣峰O·海弗里格
Owner FIRMENICH SA
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