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Tissue culture rapid-propagation method for Chinese medicinal herb Clematis finetiana

A technology of tissue culture rapid propagation and Shanketong, which is applied in the field of plant tissue culture, to achieve the effects of promoting planting and protection, consistent growth of seedlings, and low cost

Inactive Publication Date: 2019-12-27
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Akebia is mainly focused on the chemical composition and medicinal value, and there are few reports on the research on reproductive technology.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Collection of explants: The stem segment with two lateral buds in the middle and upper part of a vigorously growing Akebia plant was selected as explants in the wild in Guiping City, Guangxi. After collection, it was treated with water retention and moisturizing and brought back to the laboratory in time.

[0018] (2) Adventitious bud induction: the explants collected in step (1) back to the laboratory were rinsed under tap water for 10 hours, placed in a clean bench and sterilized in 75% ethanol solution for 30 seconds, rinsed with sterile water for 7 Blot the water with sterile filter paper after the first time, place it in 0.5% sodium hypochlorite solution for disinfection for 30 minutes, rinse it with sterile water for 10 times, then cut it into 2.5-3 cm sectioned stems and cut into 2.5-3 cm. Inoculated into the induction medium, placed in natural light at 25°C for 40 days to induce the formation of adventitious buds, and the induction rate reaches 92%. The indu...

Embodiment 2

[0024] (1) Collection of explants: The stem segment with two lateral buds in the middle and upper part of a vigorously growing Akebia plant was selected as explants in the wild in Rongxian County, Guangxi. After collection, it was treated with water retention and moisturizing and brought back to the laboratory in time.

[0025] (2) Adventitious bud induction: the explants collected in step (1) back to the laboratory were rinsed under tap water for 10 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 25 seconds, rinsed with sterile water After 5 times, dry the water with sterile filter paper, sterilize it in 0.5% sodium hypochlorite solution for 30 minutes, wash it with sterile water for 7 times, dry it with sterile filter paper, and cut it into 2.5-3cm sectioned stems and inoculated into the induction medium, placed in 25 DEG C and cultivated under natural light for 45 days to induce the formation of adventitious buds, and the induction rate r...

Embodiment 3

[0031] (1) Collection of explants: In the wild in Pubei County, Guangxi, the stem segments with two lateral buds in the middle and upper part of the vigorously growing Akebia plant were selected as explants. After collection, they were treated with water and moisture and brought back to the laboratory in time.

[0032] (2) Adventitious bud induction: the explants collected in step (1) back to the laboratory were rinsed under tap water for 10 hours, placed in an ultra-clean workbench and sterilized in 75% ethanol solution for 20 seconds, rinsed with sterile water After 5 times, dry the water with sterile filter paper, sterilize it in 0.5% sodium hypochlorite solution for 30 minutes, rinse it with sterile water for 5 times, dry it with sterile filter paper, and cut it into 2.5-3cm sectioned stems. and inoculated into the induction medium, placed in 25 DEG C and cultivated under natural light for 50 days to induce the formation of adventitious buds, and the induction rate reaches ...

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Abstract

The invention discloses a tissue culture rapid-propagation method for Chinese medicinal herb Clematis finetiana, including the steps of acquiring the explant of Clematis finetiana, and performing adventitious bud induction culture, adventitious bud enrichment culture, rooting culture and transplanting sequentially. A culture medium for the adventitious bud induction culture comprises WPM (woody plant medium), 3-4 mg / L of ZT, 3-4 mg / L of CTK, 20-30 g / L of sucrose and 3.5-4.5 g / L of agar, its pH ranges from 5.4 to 5.8. The inductivity is equal to or higher than 88%, the survival rate in transplanting is equal to or higher than 90%, and the method realizes tissue culture and rapid propagation of Clematis finetiana.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of a Chinese medicinal plant Akebia chinensis. Background technique [0002] Clematis of Ranunculaceae is a kind of plant with ornamental and medicinal value, strong climbing ability, changeable flower type, rich flower color, long flowering period, and it is also an excellent vertical greening material. Clematis finetiana is a perennial vine of the family Ranunculaceae Clematis, distributed in Yunnan, Sichuan, Guizhou (700-1200 meters), Henan Jigongshan, Hubei, Hunan (300-1200 meters), Guangdong (200- 800 meters), Guangxi, Fujian (400-1000 meters), Jiangxi (100-700 meters), Zhejiang (120-1200 meters), southern Jiangsu, south of Huaihe River in Anhui (100-900 meters). The efficacy of traditional Chinese medicine is to dispel wind and dampness, activate blood and detoxify. Control rheumatic joint ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫昭展
Owner YULIN NORMAL UNIVERSITY
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