Preparation method and application of ratio aptamer sensor for streptomycin based on electrochemistry and photoelectrochemistry dual methods

An aptamer sensor, photoelectrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve the effect of reducing interference, wide linear range and high sensitivity

Active Publication Date: 2019-12-27
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the electrochemical-photoelectrochemical dual detection method has not been reported yet.

Method used

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  • Preparation method and application of ratio aptamer sensor for streptomycin based on electrochemistry and photoelectrochemistry dual methods
  • Preparation method and application of ratio aptamer sensor for streptomycin based on electrochemistry and photoelectrochemistry dual methods
  • Preparation method and application of ratio aptamer sensor for streptomycin based on electrochemistry and photoelectrochemistry dual methods

Examples

Experimental program
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Effect test

Embodiment 1

[0028] according to figure 1 Described preparation process:

[0029] (1) Preparation of CdTe QDs

[0030] First, weigh 0.04487g of sodium borohydride and 0.0638g of tellurium powder, add 4mL of ultrapure water, pass nitrogen gas to remove oxygen for 15min, obtain a lavender solution, stir in an ice-water bath at 0°C for 8h, and obtain a precursor of sodium tellurium hydride. Then, 0.1142g CdCl 2 2.5H 2 O and 75 μL of mercaptopropionic acid were added to 50 mL of ultrapure water, and stirred for 15 min under nitrogen. The pH of the solution was adjusted to 8.5 with 1 mol / L NaOH solution. Then, quickly pour 2 mL of NaHTe precursor solution. After continuing to stir for 10 min, the mixture was transferred to a three-necked flask and refluxed at 100 °C to obtain CdTe QDs with emission spectra in the near infrared. Finally, the prepared quantum dot solution was mixed with ethanol at a ratio of 1:1, left to stand for 5 minutes, centrifuged, washed, and dispersed in ultrapure w...

Embodiment 2

[0039] (1) Preparation of CdTe QDs

[0040] First, weigh 0.04487g of sodium borohydride and 0.0638g of tellurium powder, add 4mL of ultrapure water, pass nitrogen gas to remove oxygen for 15min, obtain a lavender solution, stir in an ice-water bath at 0°C for 8h, and obtain a precursor of sodium tellurium hydride. Then, 0.1142g CdCl 2 2.5H 2 O and 75 μL of mercaptopropionic acid (MPA) were added to 50 mL of ultrapure water, and stirred for 15 min under nitrogen. The pH of the solution was adjusted to 8.5 with 1 mol / L NaOH solution. Then, quickly pour 2 mL of NaHTe precursor solution. After continuing to stir for 10 min, the mixture was transferred to a three-necked flask and refluxed at 100 °C to obtain CdTe QDs with emission spectra in the near infrared. Finally, the prepared quantum dot solution was mixed with ethanol at a ratio of 1:1, left to stand for 5 minutes, centrifuged, washed, and dispersed in ultrapure water again for later use.

[0041] The CdTe QDs solution ...

Embodiment 3

[0049] (1) Preparation of CdTe QDs

[0050] First, weigh 0.04487g of sodium borohydride and 0.0638g of tellurium powder, add 4mL of ultrapure water, pass nitrogen gas to remove oxygen for 15min, obtain a lavender solution, stir in an ice-water bath at 0°C for 8h, and obtain a precursor of sodium tellurium hydride. Then, 0.1142g CdCl 2 2.5H 2 O and 75 μL of mercaptopropionic acid were added to 50 mL of ultrapure water, and stirred for 15 min under nitrogen. The pH of the solution was adjusted to 8.5 with 1 mol / L NaOH solution. Then, quickly pour 2 mL of NaHTe precursor solution. After continuing to stir for 10 min, the mixture was transferred to a three-necked flask and refluxed at 100 °C to obtain CdTe QDs with emission spectra in the near infrared. Finally, the prepared quantum dot solution was mixed with ethanol at a ratio of 1:1, left to stand for 5 minutes, centrifuged, washed, and dispersed in ultrapure water again for later use.

[0051] The CdTe QDs solution obtain...

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Abstract

The invention belongs to the technical field of biosensor detection, and relates to preparation method and application of a ratio sensor for streptomycin based on electrochemistry and photoelectrochemistry dual methods. Specifically, the novel ratio aptamer sensor based on the electrochemistry and photoelectrochemistry dual methods is put forward for the first time for specific detection of the streptomycin. According to the preparation method and application of the ratio sensor, in the presence of the streptomycin, a methylene blue labeled streptomycin aptamer (abbreviated as MB-Apt) can be specifically combined with the streptomycin, and the MB-Apt conformation is changed, so that the MB-Apt is disengaged from the surface of an electrode, and double changes of a photoelectric chemical signal and an electrochemical signal are further caused. The linear response range of the constructed sensor to the streptomycin is 3.0*10<-8> mol/L to 1.0*10<-4> mol/L, and the detection limit is 1.0*10<-8> mol/L. The ratio aptamer sensor based on the electrochemistry and photoelectrochemistry dual methods has good selectivity and high sensitivity, higher accuracy is obtained by combining the two methods, and a novel sensing platform is provided for measuring the streptomycin in an actual sample.

Description

technical field [0001] The invention belongs to the technical field of biosensing and detection, and relates to a preparation method and application of a streptomycin electrochemical-photoelectrochemical dual-method ratio aptamer sensor, in particular to a method based on cadmium telluride quantum dots (CdTe QDs) and methylene blue (MB) Preparation method and application of streptomycin electrochemical-photoelectrochemical dual-method ratiometric sensor. Background technique [0002] Streptomycin (STR) is an aminoglycoside antibiotic widely used in animal and plant disease control, beekeeping, aquatic products and other fields. Due to its good bactericidal effect and low price, the abuse problems such as frequent drug use and excessive drug use have become increasingly prominent, resulting in drug residues in crops, water bodies and soil. Long-term intake of fruits, vegetables, and agricultural products containing streptomycin residues will directly or indirectly threaten t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/3275G01N27/305
Inventor 刘东申秀丽由天艳
Owner JIANGSU UNIV
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