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A method for efficiently treating high-ammonia nitrogen wastewater by microalgae photofermentation

A photo-fermentation and high-ammonia-nitrogen technology is applied in the field of efficient treatment of high-ammonia-nitrogen wastewater by microalgae photo-fermentation method, which can solve problems such as consumption of large and strong alkali, environmental pollution, ammonia gas escape, etc., and achieve the effect of less energy consumption

Active Publication Date: 2022-05-03
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the stable operation of the factory equipment, the stripping efficiency of ammonia nitrogen is greatly affected by the pH value and temperature, and a large amount of strong alkali is consumed, so the energy consumption and material consumption cost are very high
In addition, ammonia gas will escape during the stripping process, which will easily pollute the environment.

Method used

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  • A method for efficiently treating high-ammonia nitrogen wastewater by microalgae photofermentation
  • A method for efficiently treating high-ammonia nitrogen wastewater by microalgae photofermentation
  • A method for efficiently treating high-ammonia nitrogen wastewater by microalgae photofermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1～3

[0066] Three, embodiment 1~3 fermentation tank culture

Embodiment 1

[0067] Example 1 Fermenter two-stage culture

[0068] 2.1 High-density culture: the method is the same as Section 1.2 of the first part

[0069] 2.2 Two-stage culture in fermenter

[0070] (1) Take 1.1L of high ammonia nitrogen wastewater and mix with 2.2L of dilution water, add Basal medium mother liquor (without nitrogen) to reach the concentration of each component in Table 1 (sugar-free). Put it into a 5L glass fermenter (the total volume of fermented liquid in the fermenter is 3.5L), sterilize at 115°C for 20 minutes, take it out after sterilization and cool to room temperature, add sterilized glucose mother liquor to make the final concentration of bacterial grapes reach 10g / L, and then insert Part 2.1 The obtained seed solution. The initial inoculation cell density of Chlorella pyrenoidosa was 1×10 8 cfu / mL, every 12h, add glucose to 10g / L, the control range of pH in the fermenter is 7.0, and the light intensity, rotation speed and air flow are adjusted in gradien...

Embodiment 2

[0079] Embodiment 2 fermentor three-stage cultivation

[0080] 3.1 High-density cultivation: the method is the same as that in section 1.2 of the first part, and the cultivation steps of the first two stages of fermentor cultivation are the same as in Example 1.

[0081] 3.2 Three-stage cultivation in fermenter

[0082] (1) After the ammonium ion concentration is lower than 200mg / L (complete consumption), the fermentation broth in the 1L fermenter is released into the concentration tank, and the microalgae are collected by centrifugation to obtain the algae mud.

[0083] (2), take 1.1L of high ammonia nitrogen wastewater and mix with 2.2L of dilution water, add Basal medium mother liquor (without nitrogen) to reach 3 / 4 of the concentration of each component in Table 1 (no sugar, phosphorus element supplemented separately) . Pack into a 5L glass fermenter (total liquid volume of the fermenter is 3.5L), stir and mix well after sterilization, pour into the fermenter to contin...

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Abstract

The invention discloses a method for efficiently treating high-ammonia-nitrogen wastewater by microalgae photofermentation, comprising the following steps: S1, activating and culturing seed liquid: inoculating microalgae cells on the culture medium for activation, and then obtaining logarithmic stage seed liquid; S2, photofermentation culture: Put the prepared high-ammonia-nitrogen wastewater into a photofermentation tank, inoculate the seed liquid obtained from S1 after sterilization for cultivation, and carry out feeding in several stages of cultivation in the fermenter; Ammonium ions are depleted, microalgal biomass is harvested and purified water is obtained. The method of the present invention realizes the production of high-protein microalgae biomass while efficiently removing ammonia nitrogen in wastewater, effectively reduces the cost of wastewater treatment, realizes the comprehensive development and utilization of wastewater resources, and is suitable for wastewater rich in ammonia nitrogen in industrial and agricultural fields High-quality water purification and co-production of high-protein biomass have dual values ​​of economy and environmental protection.

Description

technical field [0001] The invention belongs to the technical field of high-ammonia-nitrogen wastewater treatment, and in particular relates to a method for efficiently treating high-ammonia-nitrogen wastewater by microalgae photofermentation. Background technique [0002] The sources of high ammonia nitrogen wastewater are mainly agriculture (animal husbandry), industrial wastewater (including chemical industry, biogas, brewing, fermentation, food, slaughtering and other industries), NH in wastewater 4 + The content is as high as 300-10000mg / L, if it is discharged into the river, it will cause great pollution to the environment. The traditional treatment technology has obvious shortcomings in cost, energy consumption and effect, and the country's environmental protection requirements are becoming increasingly stringent. The government emphasizes the need to take the path of ecological priority and green development. Therefore, it is urgent to develop a new technical syste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/32C12N1/12C12P1/02C12R1/89C02F101/16
CPCC02F3/322C12N1/12C12P1/02C02F2101/16C12R2001/89C12N1/125
Inventor 魏东余宗苡王庆科
Owner SOUTH CHINA UNIV OF TECH
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