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Sulfated xylan and preparation method and application sulfated xylan to preparation of antioxidant drugs

A xylan and sulfation technology, applied in the field of xylan modification, can solve the problems of low degree of substitution, cumbersome separation process, complex polysaccharide structure, etc.

Inactive Publication Date: 2019-12-31
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modifications such as carboxymethyl and sulfation to polysaccharide branch chains by appropriate methods can enhance the solubility and electronegativity of polysaccharides, but due to the complexity of polysaccharide structures, the degree of substitution in the prior art is low, and due to different substitution positions As a result, the biological activity varies greatly, and it is difficult to really apply it to actual production or later research; in addition, in the prior art, polyacrylamide gel, activated carbon and other technologies are mostly used for separation to obtain xylan with a low degree of polymerization or other polysaccharides for research on anti-inflammatory activity, antiviral activity, anticancer activity, anticoagulant activity, etc. On the one hand, the separation process is cumbersome, and it is necessary to carefully control the separation conditions to obtain oligosaccharides. On the other hand, Due to the characteristics of easy absorption and utilization of oligosaccharides, they have been applied to different activity studies, but for directly modified polysaccharides with a high degree of polymerization, there is no report on the application of antioxidant activities

Method used

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  • Sulfated xylan and preparation method and application sulfated xylan to preparation of antioxidant drugs
  • Sulfated xylan and preparation method and application sulfated xylan to preparation of antioxidant drugs
  • Sulfated xylan and preparation method and application sulfated xylan to preparation of antioxidant drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Preparation method of sulfated xylan.

[0034] Weigh 100mg of xylan, dissolve it in 50mL N,N-dimethylformamide in a conical flask, stir with a magnetic stirrer and raise the temperature to 80°C, add 2.5g of sulfamic acid, stir for 1 hour, stop in ice bath For the reaction, the pH was adjusted to neutral with 20% sodium hydroxide solution, dialyzed in running water for 2 days, and the sulfated xylan sample was obtained after freeze-drying.

[0035] 1. Effect of different dosages of sulfamic acid on the degree of sulfation substitution of xylan

[0036] Set the amount of sulfamic acid to 0.5g, 1g, 1.5g, 2.0g, 2.5g, and study the effect of different amounts of sulfamic acid on the degree of sulfation substitution of xylan.

[0037] Such as figure 1 As shown, the results show that when the amount of sulfamic acid added is 0.5-1.5g, the degree of substitution changes gently, and there is the highest peak at 2.5g, that is, the degree of substitution is 1.53, and the degree ...

Embodiment 2

[0048] Sulfated xylan was prepared through the optimal reaction conditions obtained in Example 1, and analyzed by infrared spectroscopy.

[0049] Specifically: weigh 2 mg of xylan and sulfated xylan prepared under optimal conditions respectively, add 100 mg of dry KBr into an agate mortar, grind evenly, and press into tablets. Carry out infrared spectrum scanning, the scanning range is 4000~400 cm-1 .

[0050] Such as Figure 5 As shown, the result shows: at 1197.63cm -1 , the absorption peak increased significantly, indicating that the sulfate radical increased, 831.20cm -1 is the characteristic absorption peak of sulfate ester bonds, indicating that sulfate groups have been linked to polysaccharide chains, and xylan has successfully prepared sulfated xylan.

Embodiment 3

[0052] Determination of degree of substitution of sulfated xylan

[0053] Weigh 3mg of the sample into a test tube, add 3mL of 1mol / L HCl, stopper, hydrolyze in a constant temperature water bath at 100°C for 6 hours, evaporate the contents to dryness at 60°C with an evaporating dish, and redissolve in 2mL of deionized water to obtain the sample hydrolysis liquid. Take 0.4mL of hydrolyzate, make up to 1.6mL with deionized water, add 8% trichloroacetic acid solution, BaCl 2 - 1.4mL each of the gelatin solutions, mix well and let stand for 15min, measure the absorbance A1 at a wavelength of 360nm.

[0054] Control group: the operation of the control group is the same as above, with 1.4mL of gelatin solution instead of BaCl 2 - Gelatin solution. The absorbance value A2 was measured at a wavelength of 360 nm.

[0055] The required absorbance A0=A1-A2, the purpose of A1-A2 is to eliminate the influence of the self-absorbing substances contained in the hydrolyzate. The quality o...

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Abstract

The invention discloses a preparation method for sulfated xylan. The preparation method comprises the following steps: mixing xylan and N,N-dimethylformamide according to a mass volume ratio of 5: (1-3), carrying out dissolving, heating the formed solution to a certain temperature under a stirring condition, then slowly adding sulfamic acid, carrying out a reaction for 1-5 h, terminating the reaction, and carrying out post-treatment to obtain sulfated xylan. The invention also discloses the sulfated xylan prepared by using the preparation method. The substitution degree of the sulfated xylan is 0.212-1.488. The invention further discloses application of the sulfated xylan to the preparation of antioxidant drugs, and the effective concentration of the sulfated xylan is 2 mg / mL. According tothe invention, sulfated xylan with different substitution degrees is prepared by adjusting reaction conditions, and the influence of different substitution degrees on different antioxidant activitiesis verified, so sulfated xylan can be selected in a targeted manner to realize different drug effects; the replacement of chemicals with sulfated xylan is made possible, and a reference basis is provided for the application of sulfated xylan to the field of medicines.

Description

technical field [0001] The invention belongs to the technical field of xylan modification, and in particular relates to a sulfated xylan, its preparation method and its application in the preparation of antioxidant drugs. Background technique [0002] Xylan is the main component of hemicellulose in plant cells. It is a complex poly-five-carbon sugar, accounting for one-third to two-fifths of the dry weight of plant cells. It is the most abundant content in nature except cellulose. The most abundant polysaccharide. Xylan has a wide range of sources and is a rich biomass resource. As a plant polysaccharide, xylan has biological activities mainly reflected in anti-oxidation, anti-tumor, and immune regulation. Based on the multiple biological activities of xylan, using xylan as a new material to replace traditional chemical products, so as to achieve the effect of reducing pollution, has always been a research hotspot for scientific researchers. However, the structure of xylan...

Claims

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Application Information

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IPC IPC(8): C08B37/14A61K31/715A61P39/06
CPCA61K31/715A61P39/06C08B37/0057
Inventor 李霞胡楠韦滢关媛李静李培骏单杨
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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