Method for extracting effective component of cotton rose hibiscus leaves based on transdermal absorption technology

A hibiscus leaf and transdermal technology, applied in the field of biomedicine, can solve problems such as large differences

Inactive Publication Date: 2020-01-03
湖州耕香生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problem that the active ingredients of hibiscus leaves in vitro are directly extracted from hibiscus leaves with solvents, which are quite different from the real system of human body digestion and absorption; the purpose of the present invention is to provide an environmentally friendly, high yield, simple process transdermal A new method of ab...

Method used

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  • Method for extracting effective component of cotton rose hibiscus leaves based on transdermal absorption technology
  • Method for extracting effective component of cotton rose hibiscus leaves based on transdermal absorption technology

Examples

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preparation example Construction

[0041] Skin preparation and pig ears were purchased from local slaughterhouses. After killing domestic pigs, cut off the ears immediately before immersing them in boiling water, shave off the hair on the outside of the ear face, use a skin grafting knife to take ear skin with a thickness of about 600 μm, store it in a freezer at -20°C, and use it up within 2 weeks.

[0042] Preparation of hibiscus leaf extract: Accurately weigh 30.00 g of hibiscus leaves, cut them into pieces, place them in a Soxhlet extractor, use 6 times the amount of 80% ethanol to refrigerate in a water bath at about 80°C for 2 hours, filter, collect the filtrate, and use the recovered filter residue for 6 times the amount of 80% ethanol was extracted twice in the same way, 2h each time, and the filtrate was collected. Combine the filtrate collected three times; put it in a rotary evaporator for concentration, the temperature is controlled at about 65°C, and when it is concentrated to about 150ml, move the...

Embodiment 1

[0043] Embodiment 1 hibiscus leaf extract aqueous solution

[0044] Raw materials: in terms of weight percentage, hibiscus leaf extract is 7%, the balance is distilled water, and the total amount is 100%; the preparation method

[0045] Method: Add hibiscus leaf extract to distilled water to make it a water homogenate.

[0046] Transdermal absorption: use pigskin to simulate human epidermis skin, cut the pigskin into 5cm*5cm size with scissors, soak in normal saline for 30 minutes, and use 30% ethanol-normal saline as a transdermal barrier Skin absorption solution, during the test, apply the precisely weighed hibiscus leaf extract aqueous solution to the processed pigskin cuticle, fill with the transdermal absorption solution, and the liquid level is just in contact with the dermis of the skin to remove air bubbles. The transdermal absorption test was carried out at a constant temperature of 37°C and a constant speed of 3800r / min. At 1h, 2h, 4h, 8h, 12h, and 24h, pipette 2mL...

Embodiment 2

[0047] Example 2 hibiscus leaf extract gel 1

[0048]Gel preparation raw materials: by weight percentage, hibiscus leaf extract 7%, propylene glycol 3%, carbomer 5%, sodium polyacrylate (NP-700) 3%, EDTA 2%, citric acid 0.4%, glycerin 35% , aluminum glycylate 2%, the balance is distilled water, the total amount is 100%.

[0049] Gel preparation method: Weigh the hibiscus leaf extract, crush it into fine powder, add it to the carbomer solution containing EDTA, mix well, add NP-700, the mixture of aluminum glycolate and glycerin, stir well, add appropriate amount Citric acid solution, stirred evenly, to obtain hibiscus leaf extract gel;

[0050] Transdermal absorption experiment: use pigskin to simulate human epidermis skin, cut the pigskin into 5cm*5cm size with scissors, then soak in normal saline for 30 minutes, as a transdermal barrier, use 30% ethanol-normal saline as the Transdermal absorption solution, during the test, apply the precisely weighed gel to the treated pigs...

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Abstract

The invention aims to provide a method for extracting an effective component of cotton rose hibiscus leaves based on a transdermal absorption technology. The method is characterized by comprising thefollowing steps: (1) gel paste is prepared, specifically, a cotton rose hibiscus leaf extract is weighed, crushed into fine powder, added into a Carbomer solution containing EDTA and evenly mixed, sodium polyacrylate and a dihydroxyaluminum aminoacetate and glycerinum mixed solution are added, even stirring is conducted, an appropriate quantity of citric acid solution is added, even stirring is conducted, and cotton rose hibiscus leaf extract gel is obtained; and (2) a transdermal absorption experiment of the cotton rose hibiscus leaf extract is conducted, specifically, pig skin is pre-treatedand then serves as a transdermal barrier, ethanol-normal saline serves as transdermal absorption fluid, the gel is smeared on treated pig skin corneum, the transdermal absorption fluid is fully injected, the liquid level is exactly in contact with a skin corium layer, bubbles are eliminated, the transdermal absorption experiment is conducted, and transdermal liquid is obtained.

Description

technical field [0001] The invention relates to a method for extracting and separating active ingredients in Hibiscus hibiscus leaves based on transdermal absorption, and the method can be applied to the fields of biomedicine and the like. Background technique [0002] Hibiscus leaves, also known as frost-resistant leaves, hibiscus flower leaves, and iron hoopsan, are the leaves of hibiscus hibiscus of the Malvaceae plant, with a slight smell and a slightly pungent taste. In summer and autumn, the leaves are cut off and dried in the sun. It must be re-dried frequently and stored in a dry and ventilated place. Remove impurities during processing, spray with water, moisten slightly, shred or chop, dry or grind into powder. Hibiscus is a deciduous shrub or small tree, 2-5m high, with dense stellate hairs and pubescence on stems, leaves, fruit stalks, bracteoles and calyx. Stem cylindrical. The leaves are alternate, oval and heart-shaped. The flowers are large, about 8 cm i...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P29/00A61K127/00
CPCA61K36/185A61P29/00
Inventor 王平陈淑婷
Owner 湖州耕香生物科技有限公司
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