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Acicular ha/pblg porous composite microcarrier material for bone tissue engineering and preparation method thereof

A technology of bone tissue engineering and microcarriers, applied in tissue regeneration, prostheses, pharmaceutical formulations, etc., can solve the problems of high preparation process requirements, shielding of biological properties of inorganic particles, uneven dispersion of inorganic substances, etc., to promote osteogenic differentiation. , good bone regeneration effect, beneficial effect of early adhesion

Active Publication Date: 2022-02-08
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the polymer materials used for tissue engineering are mainly polyesters. The disadvantage of this type of material is that the degradation products of the material will cause inflammation during the degradation process in the body.
[0008] At present, the common methods for constructing organic-inorganic composite microcarriers include blending method, grafting method, etc. The disadvantage of preparing composite microcarriers by blending method is that the inorganic matter in the composite microcarriers is not uniformly dispersed, and the gap between organic and inorganic components Weak binding
The disadvantage of the composite microcarriers obtained by the grafting method is that the preparation process is relatively high, and the biological characteristics of the inorganic particles will be partially shielded due to modification or grafting.

Method used

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  • Acicular ha/pblg porous composite microcarrier material for bone tissue engineering and preparation method thereof
  • Acicular ha/pblg porous composite microcarrier material for bone tissue engineering and preparation method thereof
  • Acicular ha/pblg porous composite microcarrier material for bone tissue engineering and preparation method thereof

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Embodiment 1

[0039] In the present embodiment, a kind of preparation method of PBLG porous microcarrier comprises the steps:

[0040] a. Poly benzyl L-glutamate (PBLG) solution preparation: in N 2 Under the atmosphere, use triethylamine as the initiator to initiate ring-opening polymerization of BLG-NCA, and obtain PBLG by precipitation with absolute ethanol; weigh 0.1-0.2g PBLG into a 25mL beaker, and then measure 10-25mL dichloromethane with a graduated cylinder Add in the beaker, stir until PBLG dissolves completely, obtain transparent solution, be poly-benzyl L-glutamate (PBLG) solution;

[0041] b. preparation of polyvinyl alcohol (PVA) aqueous solution: the PVA solid is dissolved in deionized water to obtain a PVA solution with a mass percent concentration of 0.8 to 1.0%;

[0042] c. Preparation of gelatin solution: using gelatin as a porogen, dissolving 4-6g of gelatin in 50-60mL of deionized water, stirring at 37°C until the solution is transparent and clear to obtain a gelatin so...

Embodiment 2

[0062] This embodiment is basically the same as Embodiment 1, especially in that:

[0063] In this example, the preparation method of the PBLG porous microcarrier and the preparation method of the carboxylated modified PBLG porous microcarrier are the same as those in Example 1.

[0064] In this embodiment, a method for preparing needle-shaped HA / PBLG porous composite microcarrier material for bone tissue engineering comprises the following steps:

[0065] (1) Add 100 mL of 0.25 mol / L CaCl to a 500 mL beaker 2 solution, and then add the PBLG porous microcarrier after the surface carboxylation modification of 1mL volume, obtain the first mixed liquid system;

[0066] (2) heating up to 70-80°C, and maintaining the speed of 150r / min all the time, mechanically stirring the first mixed liquid system prepared in the step (1) for 30-40min;

[0067] (3) Add 100 mL of 0.015 mol / L NaH dropwise to the first mixed liquid system that has been stirred in the step (2) at a rate of 2 to 4 m...

Embodiment 3

[0073] This embodiment is basically the same as the previous embodiment, and the special features are:

[0074] In this example, the preparation method of the PBLG porous microcarrier and the preparation method of the carboxylated modified PBLG porous microcarrier are the same as those in Example 1.

[0075] In this embodiment, a method for preparing needle-shaped HA / PBLG porous composite microcarrier material for bone tissue engineering comprises the following steps:

[0076] (1) Add 100mL of 0.05mol / L CaCl to a 500mL beaker 2 solution, and then add the PBLG porous microcarrier after the surface carboxylation modification of 1mL volume, obtain the first mixed liquid system;

[0077] (2) heating up to 70-80°C, and maintaining the speed of 150r / min all the time, mechanically stirring the first mixed liquid system prepared in the step (1) for 30-40min;

[0078] (3) Add 100 mL of 0.03 mol / L NaH dropwise to the first mixed liquid system that has been stirred in the step (2) at a...

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Abstract

The invention discloses a needle-like HA / PBLG porous composite microcarrier material for bone tissue engineering and a preparation method thereof. The PBLG porous microcarrier is firstly prepared by a double emulsion method, and the surface carboxylation is carried out to improve the The denatured PBLG porous microcarrier was used as the matrix, combined with the in-situ precipitation method, and then the needle-shaped HA / PBLG porous composite microcarrier was constructed. Cell experiments and animal experiments prove that the introduction of needle-like HA in the present invention is beneficial to the early adhesion of adipose stem cells and promotes the osteogenic differentiation of adipose stem cells. The needle-like HA / PBLG porous composite microcarrier prepared by the invention can accelerate the repair of irregular bone defects and has good bone regeneration effect.

Description

technical field [0001] The invention relates to a microcarrier material and a preparation method thereof, in particular to a porous organic-inorganic composite microcarrier material and a preparation method thereof, which are applied in the technical field of tissue engineering. Background technique [0002] Irregular bone defects caused by trauma, infection, tumor, osteonecrosis and other reasons have become a common problem in society. How to effectively treat irregular bone defects is a difficult problem in contemporary medicine. [0003] Common methods for treating bone defects include autologous bone grafting and allogeneic bone grafting. Among them, the source of autologous bone graft materials is limited, and it will cause secondary damage to the patient, while allogeneic bone graft has the risk of immune rejection and infection. Tissue engineering materials have abundant sources and various forms, and can reduce the risk of surgical infection and immune response, so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/12A61L27/18A61L27/50A61L27/56
CPCA61L27/12A61L27/18A61L27/56A61L27/50A61L2400/06A61L2430/02C08L77/04
Inventor 尹静波卜帅汪阮峰
Owner SHANGHAI UNIV
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