Polypeptide derivatives, nanofibers and their applications
A technology of nanofibers and peptide derivatives, applied in the direction of nanotechnology, nanotechnology, nanotechnology for materials and surface science, etc., can solve the problems of complex synthesis process, unfavorable long-term storage, poor water solubility, etc., and achieve simple preparation process , Improve organizational retention rate, low cost effect
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[0060] The polypeptide derivatives are synthesized by the known FMOC-short peptide solid-phase synthesis method. Specifically, the preparation method of the polypeptide derivative includes the following steps:
[0061] (1) The C-terminal of the Fmoc-amino acid is bound to the resin;
[0062] (2) removal of Fmoc protecting group, washing;
[0063] (3) The C-terminal of the next Fmoc-amino acid is coupled to the N-terminal of the amino acid or polypeptide on the resin, and washed;
[0064] (4) Repeat steps (2) to (3) until the last amino acid coupling is completed, remove the Fmoc protecting group, and wash;
[0065] (5) X-OH is coupled and washed with the N-terminal of the polypeptide on the resin;
[0066] (6) The polypeptide derivative is excised from the resin to obtain a crude product;
[0067] (7) Use high performance liquid chromatography to purify the crude product.
[0068] According to the second aspect of the present invention, the present invention provides nano...
preparation Embodiment 1
[0094] Synthesis and Preparation of Peptide Derivative Nap-FFGSSSR and Its Nanofibers
[0095] (1) Solid phase synthesis of Nap-FFGSSSR
[0096] Specific steps are as follows:
[0097] 1) Weigh 0.5mmol 2-Cl-Trt resin in a solid-phase synthesizer, add 10mL of anhydrous dichloromethane (hereinafter referred to as DCM), place on a shaker and shake for 5min to fully swell the 2-Cl-Trt resin ;
[0098] 2) Remove the DCM from the solid-phase synthesizer equipped with 2-Cl-Trt resin with ear washing ball;
[0099] 3) Dissolve 0.75 mmol of Fmoc-Arg in 10 mL of anhydrous DCM, add 0.75 mmol of DIPEA, then transfer to the above-mentioned solid-phase synthesizer, add 0.75 mmol of DIPEA, and react at room temperature for 1 h;
[0100] 4) Sealing: Remove the reaction liquid in the solid-phase synthesizer with ear washing balls, then wash with 10 mL of anhydrous DCM, 1 min each time, and wash 5 times in total. Add the prepared volume ratio of anhydrous DCM: DIPEA: methanol = 17:1:2 solut...
Embodiment 1
[0116] Activity Test of Hydrogel of Peptide Derivative Nap-FFGSSSR at the Cell Level
[0117] (1) Promoting the proliferation of mouse myoblasts (C2C12)
[0118] 1) Raise the temperature of the water bath to 37°C in advance, put the medium, serum, etc. into the water bath to preheat, and at the same time turn on the ultra-clean bench UV lamp for half an hour;
[0119] 2) Take out the frozen mouse myoblast C2C12 cells from the liquid nitrogen tank, quickly place them in a water bath at 37°C to thaw the cells, and then quickly transfer them to an ultra-clean bench for the following operations: transfer the cell-containing solution with a pipette Carefully transfer the liquid container to a centrifuge tube containing the medium, centrifuge for 3 minutes, remove the supernatant, resuspend with DMEM medium containing 10% fetal bovine serum, transfer to a petri dish, and then place it in a 37°C incubator to cultivate;
[0120] 3) Observe the state of the cells the next day. After ...
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